Transcriptomic and Coexpression Network Analyses Revealed Pine <i>Chalcone Synthase</i> Genes Associated with Pine Wood Nematode Infection
Pine wood nematode (PWN) causes serious diseases in conifers, especially pine species. To investigate the transcriptomic profiles of genes involved in pine-PWN interactions, two different pine species, namely, <i>Pinus thunbergii</i> and <i>P. massoniana</i>, were selected fo...
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MDPI AG
2021-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/20/11195 |
| _version_ | 1797514347831885824 |
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| author | Qiaoli Chen Ruizhi Zhang Danlei Li Feng Wang |
| author_facet | Qiaoli Chen Ruizhi Zhang Danlei Li Feng Wang |
| author_sort | Qiaoli Chen |
| collection | DOAJ |
| description | Pine wood nematode (PWN) causes serious diseases in conifers, especially pine species. To investigate the transcriptomic profiles of genes involved in pine-PWN interactions, two different pine species, namely, <i>Pinus thunbergii</i> and <i>P. massoniana</i>, were selected for this study. Weighted gene coexpression network analysis (WGCNA) was used to determine the relationship between changes in gene expression and the PWN population after PWN infection. PWN infection negatively affects the expression of most genes in pine trees, including plant defense-related genes such as genes related to plant hormone signal transduction, plant-pathogen interactions, and the MAPK signaling pathway in plants. However, the expression of <i>chalcone synthase</i> genes and their related genes were proportional to the changes in nematode populations, and <i>chalcone synthase</i> genes were dominant within the coexpression module enriched by genes highly correlated with the nematode population. Many genes that were closely related to <i>chalcone synthase</i> genes in the module were related to flavonoid biosynthesis, flavone and flavonol biosynthesis, and phenylpropanoid biosynthesis. Pine trees could actively adjust their defense strategies in response to changes in the number of invasive PWNs, but the sustained expression of <i>chalcone synthase</i> genes should play an important role in the inhibition of PWN infection. |
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| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T06:30:15Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e53ab9db1cf1414aa8af2ba7cd4917ca2023-11-22T18:35:26ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-10-0122201119510.3390/ijms222011195Transcriptomic and Coexpression Network Analyses Revealed Pine <i>Chalcone Synthase</i> Genes Associated with Pine Wood Nematode InfectionQiaoli Chen0Ruizhi Zhang1Danlei Li2Feng Wang3Key Laboratory of Alien Forest Pests Monitoring and Control—Heilongjiang Province, School of Forestry, Northeast Forestry University, Harbin 150040, ChinaKey Laboratory of Alien Forest Pests Monitoring and Control—Heilongjiang Province, School of Forestry, Northeast Forestry University, Harbin 150040, ChinaKey Laboratory of Alien Forest Pests Monitoring and Control—Heilongjiang Province, School of Forestry, Northeast Forestry University, Harbin 150040, ChinaKey Laboratory of Alien Forest Pests Monitoring and Control—Heilongjiang Province, School of Forestry, Northeast Forestry University, Harbin 150040, ChinaPine wood nematode (PWN) causes serious diseases in conifers, especially pine species. To investigate the transcriptomic profiles of genes involved in pine-PWN interactions, two different pine species, namely, <i>Pinus thunbergii</i> and <i>P. massoniana</i>, were selected for this study. Weighted gene coexpression network analysis (WGCNA) was used to determine the relationship between changes in gene expression and the PWN population after PWN infection. PWN infection negatively affects the expression of most genes in pine trees, including plant defense-related genes such as genes related to plant hormone signal transduction, plant-pathogen interactions, and the MAPK signaling pathway in plants. However, the expression of <i>chalcone synthase</i> genes and their related genes were proportional to the changes in nematode populations, and <i>chalcone synthase</i> genes were dominant within the coexpression module enriched by genes highly correlated with the nematode population. Many genes that were closely related to <i>chalcone synthase</i> genes in the module were related to flavonoid biosynthesis, flavone and flavonol biosynthesis, and phenylpropanoid biosynthesis. Pine trees could actively adjust their defense strategies in response to changes in the number of invasive PWNs, but the sustained expression of <i>chalcone synthase</i> genes should play an important role in the inhibition of PWN infection.https://www.mdpi.com/1422-0067/22/20/11195<i>Bursaphelenchus xylophilus</i>pine wilt diseasechalcone synthase<i>Pinus thunbergii</i><i>P. massoniana</i> |
| spellingShingle | Qiaoli Chen Ruizhi Zhang Danlei Li Feng Wang Transcriptomic and Coexpression Network Analyses Revealed Pine <i>Chalcone Synthase</i> Genes Associated with Pine Wood Nematode Infection International Journal of Molecular Sciences <i>Bursaphelenchus xylophilus</i> pine wilt disease chalcone synthase <i>Pinus thunbergii</i> <i>P. massoniana</i> |
| title | Transcriptomic and Coexpression Network Analyses Revealed Pine <i>Chalcone Synthase</i> Genes Associated with Pine Wood Nematode Infection |
| title_full | Transcriptomic and Coexpression Network Analyses Revealed Pine <i>Chalcone Synthase</i> Genes Associated with Pine Wood Nematode Infection |
| title_fullStr | Transcriptomic and Coexpression Network Analyses Revealed Pine <i>Chalcone Synthase</i> Genes Associated with Pine Wood Nematode Infection |
| title_full_unstemmed | Transcriptomic and Coexpression Network Analyses Revealed Pine <i>Chalcone Synthase</i> Genes Associated with Pine Wood Nematode Infection |
| title_short | Transcriptomic and Coexpression Network Analyses Revealed Pine <i>Chalcone Synthase</i> Genes Associated with Pine Wood Nematode Infection |
| title_sort | transcriptomic and coexpression network analyses revealed pine i chalcone synthase i genes associated with pine wood nematode infection |
| topic | <i>Bursaphelenchus xylophilus</i> pine wilt disease chalcone synthase <i>Pinus thunbergii</i> <i>P. massoniana</i> |
| url | https://www.mdpi.com/1422-0067/22/20/11195 |
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