Enzymatic degradation of extracellular DNA exposed to chlorpyrifos and chlorpyrifos-methyl in an aqueous system
The persistence of extracellular DNA (eDNA) is crucial for ensuring species diversity and ecological function in aquatic systems. However, scarce information exists about the impact of pesticides on eDNA, although they often co-exist in the aquatic environment. Using a variety of spectroscopic analy...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-11-01
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| Series: | Environment International |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0160412019316150 |
| _version_ | 1818263036996091904 |
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| author | Bing Yang Chao Qin Xiaojie Hu Kang Xia Chao Lu Fredrick Owino Gudda Zhao Ma Yanzheng Gao |
| author_facet | Bing Yang Chao Qin Xiaojie Hu Kang Xia Chao Lu Fredrick Owino Gudda Zhao Ma Yanzheng Gao |
| author_sort | Bing Yang |
| collection | DOAJ |
| description | The persistence of extracellular DNA (eDNA) is crucial for ensuring species diversity and ecological function in aquatic systems. However, scarce information exists about the impact of pesticides on eDNA, although they often co-exist in the aquatic environment. Using a variety of spectroscopic analyses, eDNA degradation and the associated alterations in DNA secondary structure was investigated by exposing DNase I to tested DNA in the presence of chlorpyrifos, a commonly used organophosphate pesticide. Molecular dynamics simulation was used to explore the weak interactions between the tested DNA and chlorpyrifos. The results indicated that chlorpyrifos significantly enhanced DNA degradation without affecting the enzyme activity of DNase I in an aqueous system. Spectroscopic experiments confirmed that chlorpyrifos and the analog chlorpyrifos-methyl could bind with DNA to cause the bases noncovalent stacking interaction. Molecular simulations further demonstrated that pesticide binding with DNA molecules caused widening of the DNA grooves and destruction of the hydrated layer, which enhanced DNA degradation. The findings presented herein provide novel insight into the genotoxicity and ecotoxicity of chlorpyrifos and chlorpyrifos-methyl, as well as their impacts on DNA persistence in aquatic environments. Keywords: Extracellular DNA, Deoxyribonuclease I (DNase I), Organophosphorus, Pesticides, Spectroscopy, Molecular dynamics |
| first_indexed | 2024-12-12T19:12:38Z |
| format | Article |
| id | doaj.art-e53b1a20195849d28117f42ef9b9fa13 |
| institution | Directory Open Access Journal |
| issn | 0160-4120 |
| language | English |
| last_indexed | 2024-12-12T19:12:38Z |
| publishDate | 2019-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Environment International |
| spelling | doaj.art-e53b1a20195849d28117f42ef9b9fa132022-12-22T00:14:48ZengElsevierEnvironment International0160-41202019-11-01132Enzymatic degradation of extracellular DNA exposed to chlorpyrifos and chlorpyrifos-methyl in an aqueous systemBing Yang0Chao Qin1Xiaojie Hu2Kang Xia3Chao Lu4Fredrick Owino Gudda5Zhao Ma6Yanzheng Gao7Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, P.R. ChinaInstitute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, P.R. ChinaInstitute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, P.R. ChinaSchool of Plant and Soil Environmental Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, United StatesInstitute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, P.R. ChinaInstitute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, P.R. ChinaInstitute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, P.R. ChinaInstitute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, P.R. China; Corresponding author.The persistence of extracellular DNA (eDNA) is crucial for ensuring species diversity and ecological function in aquatic systems. However, scarce information exists about the impact of pesticides on eDNA, although they often co-exist in the aquatic environment. Using a variety of spectroscopic analyses, eDNA degradation and the associated alterations in DNA secondary structure was investigated by exposing DNase I to tested DNA in the presence of chlorpyrifos, a commonly used organophosphate pesticide. Molecular dynamics simulation was used to explore the weak interactions between the tested DNA and chlorpyrifos. The results indicated that chlorpyrifos significantly enhanced DNA degradation without affecting the enzyme activity of DNase I in an aqueous system. Spectroscopic experiments confirmed that chlorpyrifos and the analog chlorpyrifos-methyl could bind with DNA to cause the bases noncovalent stacking interaction. Molecular simulations further demonstrated that pesticide binding with DNA molecules caused widening of the DNA grooves and destruction of the hydrated layer, which enhanced DNA degradation. The findings presented herein provide novel insight into the genotoxicity and ecotoxicity of chlorpyrifos and chlorpyrifos-methyl, as well as their impacts on DNA persistence in aquatic environments. Keywords: Extracellular DNA, Deoxyribonuclease I (DNase I), Organophosphorus, Pesticides, Spectroscopy, Molecular dynamicshttp://www.sciencedirect.com/science/article/pii/S0160412019316150 |
| spellingShingle | Bing Yang Chao Qin Xiaojie Hu Kang Xia Chao Lu Fredrick Owino Gudda Zhao Ma Yanzheng Gao Enzymatic degradation of extracellular DNA exposed to chlorpyrifos and chlorpyrifos-methyl in an aqueous system Environment International |
| title | Enzymatic degradation of extracellular DNA exposed to chlorpyrifos and chlorpyrifos-methyl in an aqueous system |
| title_full | Enzymatic degradation of extracellular DNA exposed to chlorpyrifos and chlorpyrifos-methyl in an aqueous system |
| title_fullStr | Enzymatic degradation of extracellular DNA exposed to chlorpyrifos and chlorpyrifos-methyl in an aqueous system |
| title_full_unstemmed | Enzymatic degradation of extracellular DNA exposed to chlorpyrifos and chlorpyrifos-methyl in an aqueous system |
| title_short | Enzymatic degradation of extracellular DNA exposed to chlorpyrifos and chlorpyrifos-methyl in an aqueous system |
| title_sort | enzymatic degradation of extracellular dna exposed to chlorpyrifos and chlorpyrifos methyl in an aqueous system |
| url | http://www.sciencedirect.com/science/article/pii/S0160412019316150 |
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