Leukemic Involvement Is a Common Feature in Waldenström Macroglobulinemia at Diagnosis
Waldenström Macroglobulinemia (WM) is a lymphoplasmacytic lymphoma with bone marrow (BM) involvement and IgM monoclonal gammopathy. To date, no studies have focused specifically on peripheral blood (PB) involvement. In this study, 100 patients diagnosed with WM according to the World Health Organiza...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/15/16/4152 |
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| author | Sara Montesdeoca Nieves García-Gisbert Xavier Calvo Leonor Arenillas David Román Concepción Fernández-Rodríguez Rosa Navarro Beatriz Costan María del Carmen Vela Laura Camacho Eugènia Abella Lluís Colomo Marta Salido Anna Puiggros Lourdes Florensa Blanca Espinet Beatriz Bellosillo Ana Ferrer del Álamo |
| author_facet | Sara Montesdeoca Nieves García-Gisbert Xavier Calvo Leonor Arenillas David Román Concepción Fernández-Rodríguez Rosa Navarro Beatriz Costan María del Carmen Vela Laura Camacho Eugènia Abella Lluís Colomo Marta Salido Anna Puiggros Lourdes Florensa Blanca Espinet Beatriz Bellosillo Ana Ferrer del Álamo |
| author_sort | Sara Montesdeoca |
| collection | DOAJ |
| description | Waldenström Macroglobulinemia (WM) is a lymphoplasmacytic lymphoma with bone marrow (BM) involvement and IgM monoclonal gammopathy. To date, no studies have focused specifically on peripheral blood (PB) involvement. In this study, 100 patients diagnosed with WM according to the World Health Organization (WHO) criteria were included based on the demonstration of <i>MYD88</i>mut in BM and the availability of PB multiparametric flow cytometry (MFC) analysis. Leukemic involvement by MFC was detected in 50/100 patients. A low percentage of mature small lymphocytes in PB smears was observed in only 15 cases. <i>MYD88</i>mut by AS-qPCR was detected in PB in 65/100 cases. In cases with leukemic expression by MFC, <i>MYD88</i>mut was detected in all cases, and IGH was rearranged in 44/49 cases. In 21/50 patients without PB involvement by MFC, molecular data were consistent with circulating disease (<i>MYD88</i>mut by AS-qPCR 3/50, IGH rearranged 6/50, both 12/50). Therefore, PB involvement by standard techniques was detected in 71/100 patients. <i>MYD88</i>mut was detected in PB by dPCR in 9/29 triple negative cases. Overall, 80% of the patients presented PB involvement by any technique. Our findings support the role of PB MFC in the evaluation of patients with IgM monoclonal gammopathy and provide reliable information on correlation with molecular features. The development of a feasible MFC assay may stand as an objective tool in the classification of mature B cell neoplasms presenting with IgM monoclonal gammopathy. |
| first_indexed | 2024-03-11T00:03:55Z |
| format | Article |
| id | doaj.art-e53c5b98cdae4e65ab00bdd247d95697 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-11T00:03:55Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-e53c5b98cdae4e65ab00bdd247d956972023-11-19T00:34:02ZengMDPI AGCancers2072-66942023-08-011516415210.3390/cancers15164152Leukemic Involvement Is a Common Feature in Waldenström Macroglobulinemia at DiagnosisSara Montesdeoca0Nieves García-Gisbert1Xavier Calvo2Leonor Arenillas3David Román4Concepción Fernández-Rodríguez5Rosa Navarro6Beatriz Costan7María del Carmen Vela8Laura Camacho9Eugènia Abella10Lluís Colomo11Marta Salido12Anna Puiggros13Lourdes Florensa14Blanca Espinet15Beatriz Bellosillo16Ana Ferrer del Álamo17Laboratori d’Hematologia, Servei Diagnòstic de Laboratori, Hospital Sant Joan de Déu, Esplugues de Llobregat, 08950 Barcelona, SpainLaboratori de Biologia Molecular, Servei de Patologia, Hospital del Mar, 08003 Barcelona, SpainGrup de Recerca Translacional en Neoplasies Hematològiques (GRETNHE), Hospital del Mar Research Institute (IMIM), 08003 Barcelona, SpainGrup de Recerca Translacional en Neoplasies Hematològiques (GRETNHE), Hospital del Mar Research Institute (IMIM), 08003 Barcelona, SpainGrup de Recerca Translacional en Neoplasies Hematològiques (GRETNHE), Hospital del Mar Research Institute (IMIM), 08003 Barcelona, SpainLaboratori de Biologia Molecular, Servei de Patologia, Hospital del Mar, 08003 Barcelona, SpainGrup de Recerca Translacional en Neoplasies Hematològiques (GRETNHE), Hospital del Mar Research Institute (IMIM), 08003 Barcelona, SpainGrup de Recerca Translacional en Neoplasies Hematològiques (GRETNHE), Hospital del Mar Research Institute (IMIM), 08003 Barcelona, SpainLaboratori de Biologia Molecular, Servei de Patologia, Hospital del Mar, 08003 Barcelona, SpainLaboratori de Biologia Molecular, Servei de Patologia, Hospital del Mar, 08003 Barcelona, SpainGrup de Recerca Clínica Aplicada en Neoplàsies Hematològiques, Hospital del Mar Research Institute (IMIM), 08003 Barcelona, SpainGrup de Recerca Translacional en Neoplasies Hematològiques (GRETNHE), Hospital del Mar Research Institute (IMIM), 08003 Barcelona, SpainGrup de Recerca Translacional en Neoplasies Hematològiques (GRETNHE), Hospital del Mar Research Institute (IMIM), 08003 Barcelona, SpainGrup de Recerca Translacional en Neoplasies Hematològiques (GRETNHE), Hospital del Mar Research Institute (IMIM), 08003 Barcelona, SpainGrup de Recerca Translacional en Neoplasies Hematològiques (GRETNHE), Hospital del Mar Research Institute (IMIM), 08003 Barcelona, SpainGrup de Recerca Translacional en Neoplasies Hematològiques (GRETNHE), Hospital del Mar Research Institute (IMIM), 08003 Barcelona, SpainLaboratori de Biologia Molecular, Servei de Patologia, Hospital del Mar, 08003 Barcelona, SpainGrup de Recerca Translacional en Neoplasies Hematològiques (GRETNHE), Hospital del Mar Research Institute (IMIM), 08003 Barcelona, SpainWaldenström Macroglobulinemia (WM) is a lymphoplasmacytic lymphoma with bone marrow (BM) involvement and IgM monoclonal gammopathy. To date, no studies have focused specifically on peripheral blood (PB) involvement. In this study, 100 patients diagnosed with WM according to the World Health Organization (WHO) criteria were included based on the demonstration of <i>MYD88</i>mut in BM and the availability of PB multiparametric flow cytometry (MFC) analysis. Leukemic involvement by MFC was detected in 50/100 patients. A low percentage of mature small lymphocytes in PB smears was observed in only 15 cases. <i>MYD88</i>mut by AS-qPCR was detected in PB in 65/100 cases. In cases with leukemic expression by MFC, <i>MYD88</i>mut was detected in all cases, and IGH was rearranged in 44/49 cases. In 21/50 patients without PB involvement by MFC, molecular data were consistent with circulating disease (<i>MYD88</i>mut by AS-qPCR 3/50, IGH rearranged 6/50, both 12/50). Therefore, PB involvement by standard techniques was detected in 71/100 patients. <i>MYD88</i>mut was detected in PB by dPCR in 9/29 triple negative cases. Overall, 80% of the patients presented PB involvement by any technique. Our findings support the role of PB MFC in the evaluation of patients with IgM monoclonal gammopathy and provide reliable information on correlation with molecular features. The development of a feasible MFC assay may stand as an objective tool in the classification of mature B cell neoplasms presenting with IgM monoclonal gammopathy.https://www.mdpi.com/2072-6694/15/16/4152Waldenström Macroglobulinemia (WM)peripheral blood involvementflow cytometryMFC<i>MYD88</i> L265PdPCR |
| spellingShingle | Sara Montesdeoca Nieves García-Gisbert Xavier Calvo Leonor Arenillas David Román Concepción Fernández-Rodríguez Rosa Navarro Beatriz Costan María del Carmen Vela Laura Camacho Eugènia Abella Lluís Colomo Marta Salido Anna Puiggros Lourdes Florensa Blanca Espinet Beatriz Bellosillo Ana Ferrer del Álamo Leukemic Involvement Is a Common Feature in Waldenström Macroglobulinemia at Diagnosis Cancers Waldenström Macroglobulinemia (WM) peripheral blood involvement flow cytometry MFC <i>MYD88</i> L265P dPCR |
| title | Leukemic Involvement Is a Common Feature in Waldenström Macroglobulinemia at Diagnosis |
| title_full | Leukemic Involvement Is a Common Feature in Waldenström Macroglobulinemia at Diagnosis |
| title_fullStr | Leukemic Involvement Is a Common Feature in Waldenström Macroglobulinemia at Diagnosis |
| title_full_unstemmed | Leukemic Involvement Is a Common Feature in Waldenström Macroglobulinemia at Diagnosis |
| title_short | Leukemic Involvement Is a Common Feature in Waldenström Macroglobulinemia at Diagnosis |
| title_sort | leukemic involvement is a common feature in waldenstrom macroglobulinemia at diagnosis |
| topic | Waldenström Macroglobulinemia (WM) peripheral blood involvement flow cytometry MFC <i>MYD88</i> L265P dPCR |
| url | https://www.mdpi.com/2072-6694/15/16/4152 |
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