Yiqi-Bushen-Tiaozhi Recipe Attenuated High-Fat and High-Fructose Diet Induced Nonalcoholic Steatohepatitis in Mice via Gut Microbiota
AimTo investigate the treating effect of Yiqi-Bushen-Tiaozhi (YBT) recipe on nonalcoholic steatohepatitis (NASH) mice, determine whether the outcome was associated with gut microbiota, and clarify the regulating mechanism.MethodsNASH mice were induced by high-fat and high-fructose diets (HFFD). In t...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-04-01
|
| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.824597/full |
| _version_ | 1828380637889822720 |
|---|---|
| author | Junbin Yan Junbin Yan Yunmeng Nie Yuan Liu Yuan Liu Jingya Li Jingya Li Liyan Wu Zhiyun Chen Zhiyun Chen Beihui He Beihui He |
| author_facet | Junbin Yan Junbin Yan Yunmeng Nie Yuan Liu Yuan Liu Jingya Li Jingya Li Liyan Wu Zhiyun Chen Zhiyun Chen Beihui He Beihui He |
| author_sort | Junbin Yan |
| collection | DOAJ |
| description | AimTo investigate the treating effect of Yiqi-Bushen-Tiaozhi (YBT) recipe on nonalcoholic steatohepatitis (NASH) mice, determine whether the outcome was associated with gut microbiota, and clarify the regulating mechanism.MethodsNASH mice were induced by high-fat and high-fructose diets (HFFD). In the fifth week, mice in the YBT group were orally administrated YBT (22.12g·kg-1·d-1) daily for 12 weeks. Fresh stool of mice was collected at the 16th week for fecal 16S rDNA analysis. Hepatic pathology and biochemical indicators were used to reflect the improvement of YBT on hepatic inflammation and lipid metabolism in NASH mice. Quantitative real-time PCR (qRT-PCR) was used to verify the results of PICRUSt analysis.ResultsResults of the pathological and biochemical index showed that YBT could improve NASH mice. Compared with improving inflammation and hepatocyte damage, YBT may be more focused on enhancing metabolic disorders in mice, such as increasing HDL-c level. The diversity and richness of the gut microbiota of NASH mice induced by HFFD are significantly different from the normal control (NC) group. After YBT treatment, the diversity and richness of the mice microbiota will be increased to similar NC mice. Intestinimonas, Acetatifactor, Alistipes, Intestinimonas, Acetatifactor, and Alistipes have the most significant changes in the class level. PICRUSt analysis was performed to predict genomic functions based on the 16S rDNA results and reference sequencing. The efficacy of YBT in the treatment of NASH can be achieved by regulating the diversity and richness of gut microbiota. PICRUSt analysis results showed that the most relevant function of the microbiota construction variations is α- Linolenic acid (ALA) metabolism. Results of qRT-PCR showed significant differences between groups in the expression of Fatty acid desaturase 1 (FADS1), Fatty acid desaturase 2 (FADS2), Acyl-CoA Oxidase 1 (ACOX1), and Acyl-CoA Oxidase 2 (ACOX2) related to ALA metabolism. The expression of the above genes will be inhibited in the liver and small intestine of the HFFD group mice, and the expression can be restored after YBT treatment.ConclusionYBT could treat NASH mice by improving the diversity and richness of gut microbiota and further the improvement of ALA metabolism. |
| first_indexed | 2024-12-10T04:00:33Z |
| format | Article |
| id | doaj.art-e5416d05cded463cb08a93ae3c5596ac |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-10T04:00:33Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-e5416d05cded463cb08a93ae3c5596ac2022-12-22T02:02:59ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-04-011210.3389/fcimb.2022.824597824597Yiqi-Bushen-Tiaozhi Recipe Attenuated High-Fat and High-Fructose Diet Induced Nonalcoholic Steatohepatitis in Mice via Gut MicrobiotaJunbin Yan0Junbin Yan1Yunmeng Nie2Yuan Liu3Yuan Liu4Jingya Li5Jingya Li6Liyan Wu7Zhiyun Chen8Zhiyun Chen9Beihui He10Beihui He11The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe Second Central Laboratory, Key Lab of Integrative Chinese and Western Medicine for the Diagnosis and Treatment of Circulatory Diseases of Zhejiang Province, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe Second Central Laboratory, Key Lab of Integrative Chinese and Western Medicine for the Diagnosis and Treatment of Circulatory Diseases of Zhejiang Province, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe Second Central Laboratory, Key Lab of Integrative Chinese and Western Medicine for the Diagnosis and Treatment of Circulatory Diseases of Zhejiang Province, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaDepartment of Gastroenterology, Tongde Hospital of Zhejiang Province, Hangzhou, ChinaThe First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe Second Central Laboratory, Key Lab of Integrative Chinese and Western Medicine for the Diagnosis and Treatment of Circulatory Diseases of Zhejiang Province, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe Second Central Laboratory, Key Lab of Integrative Chinese and Western Medicine for the Diagnosis and Treatment of Circulatory Diseases of Zhejiang Province, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaAimTo investigate the treating effect of Yiqi-Bushen-Tiaozhi (YBT) recipe on nonalcoholic steatohepatitis (NASH) mice, determine whether the outcome was associated with gut microbiota, and clarify the regulating mechanism.MethodsNASH mice were induced by high-fat and high-fructose diets (HFFD). In the fifth week, mice in the YBT group were orally administrated YBT (22.12g·kg-1·d-1) daily for 12 weeks. Fresh stool of mice was collected at the 16th week for fecal 16S rDNA analysis. Hepatic pathology and biochemical indicators were used to reflect the improvement of YBT on hepatic inflammation and lipid metabolism in NASH mice. Quantitative real-time PCR (qRT-PCR) was used to verify the results of PICRUSt analysis.ResultsResults of the pathological and biochemical index showed that YBT could improve NASH mice. Compared with improving inflammation and hepatocyte damage, YBT may be more focused on enhancing metabolic disorders in mice, such as increasing HDL-c level. The diversity and richness of the gut microbiota of NASH mice induced by HFFD are significantly different from the normal control (NC) group. After YBT treatment, the diversity and richness of the mice microbiota will be increased to similar NC mice. Intestinimonas, Acetatifactor, Alistipes, Intestinimonas, Acetatifactor, and Alistipes have the most significant changes in the class level. PICRUSt analysis was performed to predict genomic functions based on the 16S rDNA results and reference sequencing. The efficacy of YBT in the treatment of NASH can be achieved by regulating the diversity and richness of gut microbiota. PICRUSt analysis results showed that the most relevant function of the microbiota construction variations is α- Linolenic acid (ALA) metabolism. Results of qRT-PCR showed significant differences between groups in the expression of Fatty acid desaturase 1 (FADS1), Fatty acid desaturase 2 (FADS2), Acyl-CoA Oxidase 1 (ACOX1), and Acyl-CoA Oxidase 2 (ACOX2) related to ALA metabolism. The expression of the above genes will be inhibited in the liver and small intestine of the HFFD group mice, and the expression can be restored after YBT treatment.ConclusionYBT could treat NASH mice by improving the diversity and richness of gut microbiota and further the improvement of ALA metabolism.https://www.frontiersin.org/articles/10.3389/fcimb.2022.824597/fullnonalcoholic steatohepatitisYiqi-Bushen-Tiaozhi recipeTraditional Chinese Medicinegut microbiotaα-Linolenic acidlipid metabolism |
| spellingShingle | Junbin Yan Junbin Yan Yunmeng Nie Yuan Liu Yuan Liu Jingya Li Jingya Li Liyan Wu Zhiyun Chen Zhiyun Chen Beihui He Beihui He Yiqi-Bushen-Tiaozhi Recipe Attenuated High-Fat and High-Fructose Diet Induced Nonalcoholic Steatohepatitis in Mice via Gut Microbiota Frontiers in Cellular and Infection Microbiology nonalcoholic steatohepatitis Yiqi-Bushen-Tiaozhi recipe Traditional Chinese Medicine gut microbiota α-Linolenic acidlipid metabolism |
| title | Yiqi-Bushen-Tiaozhi Recipe Attenuated High-Fat and High-Fructose Diet Induced Nonalcoholic Steatohepatitis in Mice via Gut Microbiota |
| title_full | Yiqi-Bushen-Tiaozhi Recipe Attenuated High-Fat and High-Fructose Diet Induced Nonalcoholic Steatohepatitis in Mice via Gut Microbiota |
| title_fullStr | Yiqi-Bushen-Tiaozhi Recipe Attenuated High-Fat and High-Fructose Diet Induced Nonalcoholic Steatohepatitis in Mice via Gut Microbiota |
| title_full_unstemmed | Yiqi-Bushen-Tiaozhi Recipe Attenuated High-Fat and High-Fructose Diet Induced Nonalcoholic Steatohepatitis in Mice via Gut Microbiota |
| title_short | Yiqi-Bushen-Tiaozhi Recipe Attenuated High-Fat and High-Fructose Diet Induced Nonalcoholic Steatohepatitis in Mice via Gut Microbiota |
| title_sort | yiqi bushen tiaozhi recipe attenuated high fat and high fructose diet induced nonalcoholic steatohepatitis in mice via gut microbiota |
| topic | nonalcoholic steatohepatitis Yiqi-Bushen-Tiaozhi recipe Traditional Chinese Medicine gut microbiota α-Linolenic acidlipid metabolism |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.824597/full |
| work_keys_str_mv | AT junbinyan yiqibushentiaozhirecipeattenuatedhighfatandhighfructosedietinducednonalcoholicsteatohepatitisinmiceviagutmicrobiota AT junbinyan yiqibushentiaozhirecipeattenuatedhighfatandhighfructosedietinducednonalcoholicsteatohepatitisinmiceviagutmicrobiota AT yunmengnie yiqibushentiaozhirecipeattenuatedhighfatandhighfructosedietinducednonalcoholicsteatohepatitisinmiceviagutmicrobiota AT yuanliu yiqibushentiaozhirecipeattenuatedhighfatandhighfructosedietinducednonalcoholicsteatohepatitisinmiceviagutmicrobiota AT yuanliu yiqibushentiaozhirecipeattenuatedhighfatandhighfructosedietinducednonalcoholicsteatohepatitisinmiceviagutmicrobiota AT jingyali yiqibushentiaozhirecipeattenuatedhighfatandhighfructosedietinducednonalcoholicsteatohepatitisinmiceviagutmicrobiota AT jingyali yiqibushentiaozhirecipeattenuatedhighfatandhighfructosedietinducednonalcoholicsteatohepatitisinmiceviagutmicrobiota AT liyanwu yiqibushentiaozhirecipeattenuatedhighfatandhighfructosedietinducednonalcoholicsteatohepatitisinmiceviagutmicrobiota AT zhiyunchen yiqibushentiaozhirecipeattenuatedhighfatandhighfructosedietinducednonalcoholicsteatohepatitisinmiceviagutmicrobiota AT zhiyunchen yiqibushentiaozhirecipeattenuatedhighfatandhighfructosedietinducednonalcoholicsteatohepatitisinmiceviagutmicrobiota AT beihuihe yiqibushentiaozhirecipeattenuatedhighfatandhighfructosedietinducednonalcoholicsteatohepatitisinmiceviagutmicrobiota AT beihuihe yiqibushentiaozhirecipeattenuatedhighfatandhighfructosedietinducednonalcoholicsteatohepatitisinmiceviagutmicrobiota |