Defining the glucosylceramide population of C. elegans
Glucosylceramides (GlcCer) are lipids that impact signaling pathways, serve as critical components of cellular membranes, and act as precursors for hundreds of other complex glycolipid species. Abnormal GlcCer metabolism is linked to many diseases, including cancers, diabetes, Gaucher disease, neuro...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Physiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2023.1244158/full |
| _version_ | 1797687291680915456 |
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| author | Mark A. Xatse Carissa Perez Olsen |
| author_facet | Mark A. Xatse Carissa Perez Olsen |
| author_sort | Mark A. Xatse |
| collection | DOAJ |
| description | Glucosylceramides (GlcCer) are lipids that impact signaling pathways, serve as critical components of cellular membranes, and act as precursors for hundreds of other complex glycolipid species. Abnormal GlcCer metabolism is linked to many diseases, including cancers, diabetes, Gaucher disease, neurological disorders, and skin disorders. A key hurdle to fully understanding the role of GlcCer in disease is the development of methods to accurately detect and quantify these lipid species in a model organism. This will allow for the dissection of the role of this pool in vivo with a focus on all the individual types of GlcCer. In this review, we will discuss the analysis of the GlcCer population specifically in the nematode Caenorhabditis elegans, focusing on the mass spectrometry-based methods available for GlcCer quantification. We will also consider the combination of these approaches with genetic interrogation of GlcCer metabolic genes to define the biological role of these unique lipids. Furthermore, we will explore the implications and obstacles for future research. |
| first_indexed | 2024-03-12T01:16:08Z |
| format | Article |
| id | doaj.art-e54203c1a3fe41408d802d6dc358946f |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-03-12T01:16:08Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-e54203c1a3fe41408d802d6dc358946f2023-09-13T12:28:37ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2023-09-011410.3389/fphys.2023.12441581244158Defining the glucosylceramide population of C. elegansMark A. XatseCarissa Perez OlsenGlucosylceramides (GlcCer) are lipids that impact signaling pathways, serve as critical components of cellular membranes, and act as precursors for hundreds of other complex glycolipid species. Abnormal GlcCer metabolism is linked to many diseases, including cancers, diabetes, Gaucher disease, neurological disorders, and skin disorders. A key hurdle to fully understanding the role of GlcCer in disease is the development of methods to accurately detect and quantify these lipid species in a model organism. This will allow for the dissection of the role of this pool in vivo with a focus on all the individual types of GlcCer. In this review, we will discuss the analysis of the GlcCer population specifically in the nematode Caenorhabditis elegans, focusing on the mass spectrometry-based methods available for GlcCer quantification. We will also consider the combination of these approaches with genetic interrogation of GlcCer metabolic genes to define the biological role of these unique lipids. Furthermore, we will explore the implications and obstacles for future research.https://www.frontiersin.org/articles/10.3389/fphys.2023.1244158/fullglucosylceramide (GlcCer)sphingolipidC. elegansmass spectrometrylipidomic analysis |
| spellingShingle | Mark A. Xatse Carissa Perez Olsen Defining the glucosylceramide population of C. elegans Frontiers in Physiology glucosylceramide (GlcCer) sphingolipid C. elegans mass spectrometry lipidomic analysis |
| title | Defining the glucosylceramide population of C. elegans |
| title_full | Defining the glucosylceramide population of C. elegans |
| title_fullStr | Defining the glucosylceramide population of C. elegans |
| title_full_unstemmed | Defining the glucosylceramide population of C. elegans |
| title_short | Defining the glucosylceramide population of C. elegans |
| title_sort | defining the glucosylceramide population of c elegans |
| topic | glucosylceramide (GlcCer) sphingolipid C. elegans mass spectrometry lipidomic analysis |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2023.1244158/full |
| work_keys_str_mv | AT markaxatse definingtheglucosylceramidepopulationofcelegans AT carissaperezolsen definingtheglucosylceramidepopulationofcelegans |