Demonstrating the In Vitro and In Situ Antimicrobial Activity of Oxide Mineral Microspheres: An Innovative Technology to Be Incorporated into Porous and Nonporous Materials
The antimicrobial activity of surfaces treated with zinc and/or magnesium mineral oxide microspheres is a patented technology that has been demonstrated in vitro against bacteria and viruses. This study aims to evaluate the efficiency and sustainability of the technology in vitro, under simulation-o...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/4/1261 |
| _version_ | 1797603844891344896 |
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| author | Katia Iskandar Sophie Pecastaings Céline LeGac Sylvie Salvatico Catherine Feuillolay Mylène Guittard Loïc Marchin Marc Verelst Christine Roques |
| author_facet | Katia Iskandar Sophie Pecastaings Céline LeGac Sylvie Salvatico Catherine Feuillolay Mylène Guittard Loïc Marchin Marc Verelst Christine Roques |
| author_sort | Katia Iskandar |
| collection | DOAJ |
| description | The antimicrobial activity of surfaces treated with zinc and/or magnesium mineral oxide microspheres is a patented technology that has been demonstrated in vitro against bacteria and viruses. This study aims to evaluate the efficiency and sustainability of the technology in vitro, under simulation-of-use conditions, and in situ. The tests were undertaken in vitro according to the ISO 22196:2011, ISO 20473:2013, and NF S90-700:2019 standards with adapted parameters. Simulation-of-use tests evaluated the robustness of the activity under worst-case scenarios. The in situ tests were conducted on high-touch surfaces. The in vitro results show efficient antimicrobial activity against referenced strains with a log reduction of >2. The sustainability of this effect was time-dependent and detected at lower temperatures (20 ± 2.5 °C) and humidity (46%) conditions for variable inoculum concentrations and contact times. The simulation of use proved the microsphere’s efficiency under harsh mechanical and chemical tests. The in situ studies showed a higher than 90% reduction in CFU/25 cm<sup>2</sup> per treated surface versus the untreated surfaces, reaching a targeted value of <50 CFU/cm<sup>2</sup>. Mineral oxide microspheres can be incorporated into unlimited surface types, including medical devices, to efficiently and sustainably prevent microbial contamination. |
| first_indexed | 2024-03-11T04:38:44Z |
| format | Article |
| id | doaj.art-e54221ca4c1b45a487506ef7682b081b |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-11T04:38:44Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-e54221ca4c1b45a487506ef7682b081b2023-11-17T20:55:01ZengMDPI AGPharmaceutics1999-49232023-04-01154126110.3390/pharmaceutics15041261Demonstrating the In Vitro and In Situ Antimicrobial Activity of Oxide Mineral Microspheres: An Innovative Technology to Be Incorporated into Porous and Nonporous MaterialsKatia Iskandar0Sophie Pecastaings1Céline LeGac2Sylvie Salvatico3Catherine Feuillolay4Mylène Guittard5Loïc Marchin6Marc Verelst7Christine Roques8Department of Pharmacy, School of Pharmacy, Lebanese International University, Bekaa P.O. Box 146404, LebanonLaboratoire de Génie Chimique, Faculté de Pharmacie, Université de Toulouse, CNRS, INPT, UPS, 31062 Toulouse, FranceFONDEREPHAR, Faculté de Pharmacie, 31062 Toulouse, FranceFONDEREPHAR, Faculté de Pharmacie, 31062 Toulouse, FranceFONDEREPHAR, Faculté de Pharmacie, 31062 Toulouse, FrancePylote SAS, 22 Avenue de la Mouyssaguèse, 31280 Drémil-Lafage, FrancePylote SAS, 22 Avenue de la Mouyssaguèse, 31280 Drémil-Lafage, FranceCEMES, UPR CNRS 8011, 29 Rue Jeanne Marvig, CEDEX, 31055 Toulouse, FranceLaboratoire de Génie Chimique, Faculté de Pharmacie, Université de Toulouse, CNRS, INPT, UPS, 31062 Toulouse, FranceThe antimicrobial activity of surfaces treated with zinc and/or magnesium mineral oxide microspheres is a patented technology that has been demonstrated in vitro against bacteria and viruses. This study aims to evaluate the efficiency and sustainability of the technology in vitro, under simulation-of-use conditions, and in situ. The tests were undertaken in vitro according to the ISO 22196:2011, ISO 20473:2013, and NF S90-700:2019 standards with adapted parameters. Simulation-of-use tests evaluated the robustness of the activity under worst-case scenarios. The in situ tests were conducted on high-touch surfaces. The in vitro results show efficient antimicrobial activity against referenced strains with a log reduction of >2. The sustainability of this effect was time-dependent and detected at lower temperatures (20 ± 2.5 °C) and humidity (46%) conditions for variable inoculum concentrations and contact times. The simulation of use proved the microsphere’s efficiency under harsh mechanical and chemical tests. The in situ studies showed a higher than 90% reduction in CFU/25 cm<sup>2</sup> per treated surface versus the untreated surfaces, reaching a targeted value of <50 CFU/cm<sup>2</sup>. Mineral oxide microspheres can be incorporated into unlimited surface types, including medical devices, to efficiently and sustainably prevent microbial contamination.https://www.mdpi.com/1999-4923/15/4/1261oxide mineral microspheresantimicrobial surfacesimulation of use conditionsin situ |
| spellingShingle | Katia Iskandar Sophie Pecastaings Céline LeGac Sylvie Salvatico Catherine Feuillolay Mylène Guittard Loïc Marchin Marc Verelst Christine Roques Demonstrating the In Vitro and In Situ Antimicrobial Activity of Oxide Mineral Microspheres: An Innovative Technology to Be Incorporated into Porous and Nonporous Materials Pharmaceutics oxide mineral microspheres antimicrobial surface simulation of use conditions in situ |
| title | Demonstrating the In Vitro and In Situ Antimicrobial Activity of Oxide Mineral Microspheres: An Innovative Technology to Be Incorporated into Porous and Nonporous Materials |
| title_full | Demonstrating the In Vitro and In Situ Antimicrobial Activity of Oxide Mineral Microspheres: An Innovative Technology to Be Incorporated into Porous and Nonporous Materials |
| title_fullStr | Demonstrating the In Vitro and In Situ Antimicrobial Activity of Oxide Mineral Microspheres: An Innovative Technology to Be Incorporated into Porous and Nonporous Materials |
| title_full_unstemmed | Demonstrating the In Vitro and In Situ Antimicrobial Activity of Oxide Mineral Microspheres: An Innovative Technology to Be Incorporated into Porous and Nonporous Materials |
| title_short | Demonstrating the In Vitro and In Situ Antimicrobial Activity of Oxide Mineral Microspheres: An Innovative Technology to Be Incorporated into Porous and Nonporous Materials |
| title_sort | demonstrating the in vitro and in situ antimicrobial activity of oxide mineral microspheres an innovative technology to be incorporated into porous and nonporous materials |
| topic | oxide mineral microspheres antimicrobial surface simulation of use conditions in situ |
| url | https://www.mdpi.com/1999-4923/15/4/1261 |
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