Cellular Localization of Two <i>Rickettsia</i> Symbionts in the Digestive System and within the Ovaries of the Mirid Bug, <i>Macrolophous pygmaeus</i>
Bacterial symbionts in arthropods are common, vary in their effects, and can dramatically influence the outcome of biological control efforts. <i>Macrolophus pygmaeus</i> (Heteroptera: Miridae), a key component of biological control programs, is mainly predaceous but may also display phy...
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MDPI AG
2020-08-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/8/530 |
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| author | Maria Dally Maya Lalzar Eduard Belausov Yuval Gottlieb Moshe Coll Einat Zchori-Fein |
| author_facet | Maria Dally Maya Lalzar Eduard Belausov Yuval Gottlieb Moshe Coll Einat Zchori-Fein |
| author_sort | Maria Dally |
| collection | DOAJ |
| description | Bacterial symbionts in arthropods are common, vary in their effects, and can dramatically influence the outcome of biological control efforts. <i>Macrolophus pygmaeus</i> (Heteroptera: Miridae), a key component of biological control programs, is mainly predaceous but may also display phytophagy. <i>M. pygmaeus</i> hosts symbiotic <i>Wolbachia</i>, which induce cytoplasmic incompatibility, and two <i>Rickettsia</i> species, <i>R. bellii</i> and <i>R. limoniae</i>, which are found in all individuals tested. To test possible involvement of the two <i>Rickettsia</i> species in the feeding habits of <i>M. pygmaeus</i>, we first showed that the microbiome of the insect is dominated by these three symbionts, and later described the distribution pattern of the two <i>Rickettsia</i> species in its digestive system. Although both <i>Rickettsia</i> species were located in certain gut bacteriocyes, in caeca and in Malpighian tubules of both sexes, each species has a unique cellular occupancy pattern and specific distribution along digestive system compartments. Infrequently, both species were found in a cell. In females, both <i>Rickettsia</i> species were detected in the germarium, the apical end of the ovarioles within the ovaries, but not in oocytes. Although the cause for these <i>Rickettsia</i> distribution patterns is yet unknown, it is likely linked to host nutrition while feeding on prey or plants. |
| first_indexed | 2024-03-10T17:28:55Z |
| format | Article |
| id | doaj.art-e543f22a0c3348568e3130b6cd49f63d |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T17:28:55Z |
| publishDate | 2020-08-01 |
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| record_format | Article |
| series | Insects |
| spelling | doaj.art-e543f22a0c3348568e3130b6cd49f63d2023-11-20T10:05:37ZengMDPI AGInsects2075-44502020-08-0111853010.3390/insects11080530Cellular Localization of Two <i>Rickettsia</i> Symbionts in the Digestive System and within the Ovaries of the Mirid Bug, <i>Macrolophous pygmaeus</i>Maria Dally0Maya Lalzar1Eduard Belausov2Yuval Gottlieb3Moshe Coll4Einat Zchori-Fein5Department of Entomology, RH Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, POB 12, Rehovot 7610001, IsraelBioinformatics Service Unit, University of Haifa, Haifa 3498838, IsraelThe Institute of Plant Sciences, The Volcani Center, ARO, HaMaccabim Road, Rishon LeZion 7528809, IsraelKoret School of Veterinary Medicine, The Hebrew University of Jerusalem, POB 12, Rehovot 76100, IsraelDepartment of Entomology, RH Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, POB 12, Rehovot 7610001, IsraelDepartment of Entomology, Newe-Ya’ar Research Center, ARO, Ramat-Yishay 30095, IsraelBacterial symbionts in arthropods are common, vary in their effects, and can dramatically influence the outcome of biological control efforts. <i>Macrolophus pygmaeus</i> (Heteroptera: Miridae), a key component of biological control programs, is mainly predaceous but may also display phytophagy. <i>M. pygmaeus</i> hosts symbiotic <i>Wolbachia</i>, which induce cytoplasmic incompatibility, and two <i>Rickettsia</i> species, <i>R. bellii</i> and <i>R. limoniae</i>, which are found in all individuals tested. To test possible involvement of the two <i>Rickettsia</i> species in the feeding habits of <i>M. pygmaeus</i>, we first showed that the microbiome of the insect is dominated by these three symbionts, and later described the distribution pattern of the two <i>Rickettsia</i> species in its digestive system. Although both <i>Rickettsia</i> species were located in certain gut bacteriocyes, in caeca and in Malpighian tubules of both sexes, each species has a unique cellular occupancy pattern and specific distribution along digestive system compartments. Infrequently, both species were found in a cell. In females, both <i>Rickettsia</i> species were detected in the germarium, the apical end of the ovarioles within the ovaries, but not in oocytes. Although the cause for these <i>Rickettsia</i> distribution patterns is yet unknown, it is likely linked to host nutrition while feeding on prey or plants.https://www.mdpi.com/2075-4450/11/8/530bacteriocytebacterial microbiomeFISHomnivory |
| spellingShingle | Maria Dally Maya Lalzar Eduard Belausov Yuval Gottlieb Moshe Coll Einat Zchori-Fein Cellular Localization of Two <i>Rickettsia</i> Symbionts in the Digestive System and within the Ovaries of the Mirid Bug, <i>Macrolophous pygmaeus</i> Insects bacteriocyte bacterial microbiome FISH omnivory |
| title | Cellular Localization of Two <i>Rickettsia</i> Symbionts in the Digestive System and within the Ovaries of the Mirid Bug, <i>Macrolophous pygmaeus</i> |
| title_full | Cellular Localization of Two <i>Rickettsia</i> Symbionts in the Digestive System and within the Ovaries of the Mirid Bug, <i>Macrolophous pygmaeus</i> |
| title_fullStr | Cellular Localization of Two <i>Rickettsia</i> Symbionts in the Digestive System and within the Ovaries of the Mirid Bug, <i>Macrolophous pygmaeus</i> |
| title_full_unstemmed | Cellular Localization of Two <i>Rickettsia</i> Symbionts in the Digestive System and within the Ovaries of the Mirid Bug, <i>Macrolophous pygmaeus</i> |
| title_short | Cellular Localization of Two <i>Rickettsia</i> Symbionts in the Digestive System and within the Ovaries of the Mirid Bug, <i>Macrolophous pygmaeus</i> |
| title_sort | cellular localization of two i rickettsia i symbionts in the digestive system and within the ovaries of the mirid bug i macrolophous pygmaeus i |
| topic | bacteriocyte bacterial microbiome FISH omnivory |
| url | https://www.mdpi.com/2075-4450/11/8/530 |
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