Fluid shear stress impacts ovarian cancer cell viability, subcellular organization, and promotes genomic instability.
Ovarian cancer cells are exposed to physical stress in the peritoneal cavity during both tumor growth and dissemination. Ascites build-up in metastatic ovarian cancer further increases the exposure to fluid shear stress. Here, we used a murine, in vitro ovarian cancer progression model in parallel w...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5864000?pdf=render |
| _version_ | 1819117827663069184 |
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| author | Alexandra R Hyler Nicolaas C Baudoin Megan S Brown Mark A Stremler Daniela Cimini Rafael V Davalos Eva M Schmelz |
| author_facet | Alexandra R Hyler Nicolaas C Baudoin Megan S Brown Mark A Stremler Daniela Cimini Rafael V Davalos Eva M Schmelz |
| author_sort | Alexandra R Hyler |
| collection | DOAJ |
| description | Ovarian cancer cells are exposed to physical stress in the peritoneal cavity during both tumor growth and dissemination. Ascites build-up in metastatic ovarian cancer further increases the exposure to fluid shear stress. Here, we used a murine, in vitro ovarian cancer progression model in parallel with immortalized human cells to investigate how ovarian cancer cells of increasing aggressiveness respond to [Formula: see text] of fluid-induced shear stress. This biophysical stimulus significantly reduced cell viability in all cells exposed, independent of disease stage. Fluid shear stress induced spheroid formation and altered cytoskeleton organization in more tumorigenic cell lines. While benign ovarian cells appeared to survive in higher numbers under the influence of fluid shear stress, they exhibited severe morphological changes and chromosomal instability. These results suggest that exposure of benign cells to low magnitude fluid shear stress can induce phenotypic changes that are associated with transformation and ovarian cancer progression. Moreover, exposure of tumorigenic cells to fluid shear stress enhanced anchorage-independent survival, suggesting a role in promoting invasion and metastasis. |
| first_indexed | 2024-12-22T05:39:10Z |
| format | Article |
| id | doaj.art-e5456308defb48828ccc97d277a6e9e5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T05:39:10Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e5456308defb48828ccc97d277a6e9e52022-12-21T18:37:15ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01133e019417010.1371/journal.pone.0194170Fluid shear stress impacts ovarian cancer cell viability, subcellular organization, and promotes genomic instability.Alexandra R HylerNicolaas C BaudoinMegan S BrownMark A StremlerDaniela CiminiRafael V DavalosEva M SchmelzOvarian cancer cells are exposed to physical stress in the peritoneal cavity during both tumor growth and dissemination. Ascites build-up in metastatic ovarian cancer further increases the exposure to fluid shear stress. Here, we used a murine, in vitro ovarian cancer progression model in parallel with immortalized human cells to investigate how ovarian cancer cells of increasing aggressiveness respond to [Formula: see text] of fluid-induced shear stress. This biophysical stimulus significantly reduced cell viability in all cells exposed, independent of disease stage. Fluid shear stress induced spheroid formation and altered cytoskeleton organization in more tumorigenic cell lines. While benign ovarian cells appeared to survive in higher numbers under the influence of fluid shear stress, they exhibited severe morphological changes and chromosomal instability. These results suggest that exposure of benign cells to low magnitude fluid shear stress can induce phenotypic changes that are associated with transformation and ovarian cancer progression. Moreover, exposure of tumorigenic cells to fluid shear stress enhanced anchorage-independent survival, suggesting a role in promoting invasion and metastasis.http://europepmc.org/articles/PMC5864000?pdf=render |
| spellingShingle | Alexandra R Hyler Nicolaas C Baudoin Megan S Brown Mark A Stremler Daniela Cimini Rafael V Davalos Eva M Schmelz Fluid shear stress impacts ovarian cancer cell viability, subcellular organization, and promotes genomic instability. PLoS ONE |
| title | Fluid shear stress impacts ovarian cancer cell viability, subcellular organization, and promotes genomic instability. |
| title_full | Fluid shear stress impacts ovarian cancer cell viability, subcellular organization, and promotes genomic instability. |
| title_fullStr | Fluid shear stress impacts ovarian cancer cell viability, subcellular organization, and promotes genomic instability. |
| title_full_unstemmed | Fluid shear stress impacts ovarian cancer cell viability, subcellular organization, and promotes genomic instability. |
| title_short | Fluid shear stress impacts ovarian cancer cell viability, subcellular organization, and promotes genomic instability. |
| title_sort | fluid shear stress impacts ovarian cancer cell viability subcellular organization and promotes genomic instability |
| url | http://europepmc.org/articles/PMC5864000?pdf=render |
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