Recovery of value-added anthocyanins from mulberry by a cation exchange chromatography
Anthocyanins are often targets in polyphenol analysis. However, it's hard to effectively separate anthocyanin from copigments such as phenolic acid and flavanols due to their similar structure. Thus, a cation exchange chromatography with 001 × 7 has been developed, which is available for anthoc...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2022-01-01
|
Series: | Current Research in Food Science |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2665927122001393 |
_version_ | 1828168503934320640 |
---|---|
author | Zhiwei Liao Xuan Zhang Xi Chen Maurizio Battino Francesca Giampieri Weibin Bai Lingmin Tian |
author_facet | Zhiwei Liao Xuan Zhang Xi Chen Maurizio Battino Francesca Giampieri Weibin Bai Lingmin Tian |
author_sort | Zhiwei Liao |
collection | DOAJ |
description | Anthocyanins are often targets in polyphenol analysis. However, it's hard to effectively separate anthocyanin from copigments such as phenolic acid and flavanols due to their similar structure. Thus, a cation exchange chromatography with 001 × 7 has been developed, which is available for anthocyanins isolation both on a small and large scale. The optimal process condition of anthocyanins isolation was determined. Compared to the macroporous adsorbent resins and Strong Cation Exchange resin (SCX), 001X7 shows greater economic advantages in large-scale purification of anthocyanins. More than 95% purity of the anthocyanin fraction can be achieved through this approach. This method shows a path to provide large quantities of copigments-free anthocyanins from mulberry polyphenols for the further study of its biological effects and may be extended to other analytical methods of polyphenol isolation from other plant materials. |
first_indexed | 2024-04-12T02:35:53Z |
format | Article |
id | doaj.art-e5467dee3bd4421c8bbbf3b1bc9f41eb |
institution | Directory Open Access Journal |
issn | 2665-9271 |
language | English |
last_indexed | 2024-04-12T02:35:53Z |
publishDate | 2022-01-01 |
publisher | Elsevier |
record_format | Article |
series | Current Research in Food Science |
spelling | doaj.art-e5467dee3bd4421c8bbbf3b1bc9f41eb2022-12-22T03:51:35ZengElsevierCurrent Research in Food Science2665-92712022-01-01514451451Recovery of value-added anthocyanins from mulberry by a cation exchange chromatographyZhiwei Liao0Xuan Zhang1Xi Chen2Maurizio Battino3Francesca Giampieri4Weibin Bai5Lingmin Tian6Department of Food Science and Engineering, Institute of Food Safety and Nutrition, Guangdong Engineering Technology Center of Food Safety Molecular Rapid Detection, Jinan University, Guangzhou, ChinaDepartment of Food Science and Engineering, Institute of Food Safety and Nutrition, Guangdong Engineering Technology Center of Food Safety Molecular Rapid Detection, Jinan University, Guangzhou, ChinaKey Laboratory of Regenerative Medicine, Ministry of Education, Department of Developmental and Regenerative Biology, Jinan University, Guangzhou, ChinaDepartment of Clinical Sciences, Polytechnic University of Marche, 60131, Ancona, Italy; Research Group on Foods, Nutritional Biochemistry and Health, Universidad Europea Del Atlántico, 39011, Santander, SpainResearch Group on Foods, Nutritional Biochemistry and Health, Universidad Europea Del Atlántico, 39011, Santander, Spain; Department of Biochemistry, Faculty of Sciences, King Abdulaziz University, Jeddah, 80200, Saudi ArabiaDepartment of Food Science and Engineering, Institute of Food Safety and Nutrition, Guangdong Engineering Technology Center of Food Safety Molecular Rapid Detection, Jinan University, Guangzhou, China; Corresponding author.Department of Food Science and Engineering, Institute of Food Safety and Nutrition, Guangdong Engineering Technology Center of Food Safety Molecular Rapid Detection, Jinan University, Guangzhou, ChinaAnthocyanins are often targets in polyphenol analysis. However, it's hard to effectively separate anthocyanin from copigments such as phenolic acid and flavanols due to their similar structure. Thus, a cation exchange chromatography with 001 × 7 has been developed, which is available for anthocyanins isolation both on a small and large scale. The optimal process condition of anthocyanins isolation was determined. Compared to the macroporous adsorbent resins and Strong Cation Exchange resin (SCX), 001X7 shows greater economic advantages in large-scale purification of anthocyanins. More than 95% purity of the anthocyanin fraction can be achieved through this approach. This method shows a path to provide large quantities of copigments-free anthocyanins from mulberry polyphenols for the further study of its biological effects and may be extended to other analytical methods of polyphenol isolation from other plant materials.http://www.sciencedirect.com/science/article/pii/S2665927122001393Column chromatography001X7AnthocyaninsCopigmentsIsolationScale-up |
spellingShingle | Zhiwei Liao Xuan Zhang Xi Chen Maurizio Battino Francesca Giampieri Weibin Bai Lingmin Tian Recovery of value-added anthocyanins from mulberry by a cation exchange chromatography Current Research in Food Science Column chromatography 001X7 Anthocyanins Copigments Isolation Scale-up |
title | Recovery of value-added anthocyanins from mulberry by a cation exchange chromatography |
title_full | Recovery of value-added anthocyanins from mulberry by a cation exchange chromatography |
title_fullStr | Recovery of value-added anthocyanins from mulberry by a cation exchange chromatography |
title_full_unstemmed | Recovery of value-added anthocyanins from mulberry by a cation exchange chromatography |
title_short | Recovery of value-added anthocyanins from mulberry by a cation exchange chromatography |
title_sort | recovery of value added anthocyanins from mulberry by a cation exchange chromatography |
topic | Column chromatography 001X7 Anthocyanins Copigments Isolation Scale-up |
url | http://www.sciencedirect.com/science/article/pii/S2665927122001393 |
work_keys_str_mv | AT zhiweiliao recoveryofvalueaddedanthocyaninsfrommulberrybyacationexchangechromatography AT xuanzhang recoveryofvalueaddedanthocyaninsfrommulberrybyacationexchangechromatography AT xichen recoveryofvalueaddedanthocyaninsfrommulberrybyacationexchangechromatography AT mauriziobattino recoveryofvalueaddedanthocyaninsfrommulberrybyacationexchangechromatography AT francescagiampieri recoveryofvalueaddedanthocyaninsfrommulberrybyacationexchangechromatography AT weibinbai recoveryofvalueaddedanthocyaninsfrommulberrybyacationexchangechromatography AT lingmintian recoveryofvalueaddedanthocyaninsfrommulberrybyacationexchangechromatography |