Repression by the Arabidopsis TOPLESS corepressor requires association with the core mediator complex
The plant corepressor TOPLESS (TPL) is recruited to a large number of loci that are selectively induced in response to developmental or environmental cues, yet the mechanisms by which it inhibits expression in the absence of these stimuli are poorly understood. Previously, we had used the N-terminus...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2021-06-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/66739 |
| _version_ | 1811180575820611584 |
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| author | Alexander R Leydon Wei Wang Hardik P Gala Sabrina Gilmour Samuel Juarez-Solis Mollye L Zahler Joseph E Zemke Ning Zheng Jennifer L Nemhauser |
| author_facet | Alexander R Leydon Wei Wang Hardik P Gala Sabrina Gilmour Samuel Juarez-Solis Mollye L Zahler Joseph E Zemke Ning Zheng Jennifer L Nemhauser |
| author_sort | Alexander R Leydon |
| collection | DOAJ |
| description | The plant corepressor TOPLESS (TPL) is recruited to a large number of loci that are selectively induced in response to developmental or environmental cues, yet the mechanisms by which it inhibits expression in the absence of these stimuli are poorly understood. Previously, we had used the N-terminus of Arabidopsis thaliana TPL to enable repression of a synthetic auxin response circuit in Saccharomyces cerevisiae (yeast). Here, we leveraged the yeast system to interrogate the relationship between TPL structure and function, specifically scanning for repression domains. We identified a potent repression domain in Helix 8 located within the CRA domain, which directly interacted with the Mediator middle module subunits Med21 and Med10. Interactions between TPL and Mediator were required to fully repress transcription in both yeast and plants. In contrast, we found that multimer formation, a conserved feature of many corepressors, had minimal influence on the repression strength of TPL. |
| first_indexed | 2024-04-11T09:05:31Z |
| format | Article |
| id | doaj.art-e549ac4846094348b6a07be89e102be7 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:05:31Z |
| publishDate | 2021-06-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-e549ac4846094348b6a07be89e102be72022-12-22T04:32:39ZengeLife Sciences Publications LtdeLife2050-084X2021-06-011010.7554/eLife.66739Repression by the Arabidopsis TOPLESS corepressor requires association with the core mediator complexAlexander R Leydon0https://orcid.org/0000-0003-3034-1482Wei Wang1Hardik P Gala2Sabrina Gilmour3Samuel Juarez-Solis4Mollye L Zahler5Joseph E Zemke6Ning Zheng7Jennifer L Nemhauser8https://orcid.org/0000-0002-8909-735XDepartment of Biology, University of Washington, Seattle, United StatesDepartment of Pharmacology, Seattle, United StatesDepartment of Biology, University of Washington, Seattle, United StatesDepartment of Biology, University of Washington, Seattle, United StatesDepartment of Biology, University of Washington, Seattle, United StatesDepartment of Biology, University of Washington, Seattle, United StatesDepartment of Biology, University of Washington, Seattle, United StatesDepartment of Pharmacology, Seattle, United States; Howard Hughes Medical Institute, University of Washington, Seattle, United StatesDepartment of Biology, University of Washington, Seattle, United StatesThe plant corepressor TOPLESS (TPL) is recruited to a large number of loci that are selectively induced in response to developmental or environmental cues, yet the mechanisms by which it inhibits expression in the absence of these stimuli are poorly understood. Previously, we had used the N-terminus of Arabidopsis thaliana TPL to enable repression of a synthetic auxin response circuit in Saccharomyces cerevisiae (yeast). Here, we leveraged the yeast system to interrogate the relationship between TPL structure and function, specifically scanning for repression domains. We identified a potent repression domain in Helix 8 located within the CRA domain, which directly interacted with the Mediator middle module subunits Med21 and Med10. Interactions between TPL and Mediator were required to fully repress transcription in both yeast and plants. In contrast, we found that multimer formation, a conserved feature of many corepressors, had minimal influence on the repression strength of TPL.https://elifesciences.org/articles/66739corepressorsmediatorTOPLESStranscriptional repression |
| spellingShingle | Alexander R Leydon Wei Wang Hardik P Gala Sabrina Gilmour Samuel Juarez-Solis Mollye L Zahler Joseph E Zemke Ning Zheng Jennifer L Nemhauser Repression by the Arabidopsis TOPLESS corepressor requires association with the core mediator complex eLife corepressors mediator TOPLESS transcriptional repression |
| title | Repression by the Arabidopsis TOPLESS corepressor requires association with the core mediator complex |
| title_full | Repression by the Arabidopsis TOPLESS corepressor requires association with the core mediator complex |
| title_fullStr | Repression by the Arabidopsis TOPLESS corepressor requires association with the core mediator complex |
| title_full_unstemmed | Repression by the Arabidopsis TOPLESS corepressor requires association with the core mediator complex |
| title_short | Repression by the Arabidopsis TOPLESS corepressor requires association with the core mediator complex |
| title_sort | repression by the arabidopsis topless corepressor requires association with the core mediator complex |
| topic | corepressors mediator TOPLESS transcriptional repression |
| url | https://elifesciences.org/articles/66739 |
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