Molecular Detection of Avian Metapneumovirus in Semnan BroilerFarms
BACKGROUND: Avian Metapneumovirus (AMPV) causes mild to acute contagious infection of the upper respiratory tract in turkey and chicken with different mortality rate. OBJECTIVES: This study was aimed to molecular detection and subtyping of AMPV infection in broiler flocks using RT-PCR method in Sem...
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| Format: | Article |
| Language: | English |
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University of Tehran
2021-02-01
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| Series: | Iranian Journal of Veterinary Medicine |
| Subjects: | |
| Online Access: | https://ijvm.ut.ac.ir/article_81086_53465ca05e5001a208735c11b8ab420f.pdf |
| _version_ | 1819122145064648704 |
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| author | Amir Darebaghi Seyed Hesamoddin Emadi Chashmi Khatereh Kafshdozan Hossein hossein |
| author_facet | Amir Darebaghi Seyed Hesamoddin Emadi Chashmi Khatereh Kafshdozan Hossein hossein |
| author_sort | Amir Darebaghi |
| collection | DOAJ |
| description | BACKGROUND: Avian Metapneumovirus (AMPV) causes mild to acute contagious infection of the upper respiratory tract in turkey and chicken with different mortality rate. OBJECTIVES: This study was aimed to molecular detection and subtyping of AMPV infection in broiler flocks using RT-PCR method in Semnan province on samples from 2016 to2020. METHODS: Sampling was carried out from the upper part of the trachea, choana, and sinuses of broiler chickens from the 85 broiler flocks. All flocks were more than 3 weeks of age. In total 10 swabs were taken from each flock, while each 5 were pooled as one sample (total two samples per flock). The samples were transferred to the laboratory for RNA extraction and RT-PCR amplification. RESULTS: Out of 85 tested broiler flocks, 30 (35.3%) were positive for AMPV using the Nd/Nx primer set. In addition, 28 positive samples were found to be of subtype B using the Ga/G12 primer set and 2 remaining positive samples were non-subtype B, probably A, C or D subtypes. CONCLUSIONS: Since AMPV vaccination was not performed in Semnan province, it can be concluded that some cases were infected with the natural viruses. Therefore, vaccination could be effective in controlling AMPV-induced respiratory distress. |
| first_indexed | 2024-12-22T06:47:48Z |
| format | Article |
| id | doaj.art-e54ae4383ab74847ad1e57850e70d13b |
| institution | Directory Open Access Journal |
| issn | 2251-8894 2252-0554 |
| language | English |
| last_indexed | 2024-12-22T06:47:48Z |
| publishDate | 2021-02-01 |
| publisher | University of Tehran |
| record_format | Article |
| series | Iranian Journal of Veterinary Medicine |
| spelling | doaj.art-e54ae4383ab74847ad1e57850e70d13b2022-12-21T18:35:15ZengUniversity of TehranIranian Journal of Veterinary Medicine2251-88942252-05542021-02-01151273410.22059/ijvm.2020.302556.100508781086Molecular Detection of Avian Metapneumovirus in Semnan BroilerFarmsAmir Darebaghi0Seyed Hesamoddin Emadi Chashmi1Khatereh Kafshdozan2Hossein hossein3Graduated from the Faculty of Veterinary Medicine, Semnan University, Semnan, IranDepartment of Clinical Sciences, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran;Department of Pathobiology, Faculty of Veterinary Medicine, Semnan University, Semnan, IranDepartment of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Karaj Branch, Karaj, IranBACKGROUND: Avian Metapneumovirus (AMPV) causes mild to acute contagious infection of the upper respiratory tract in turkey and chicken with different mortality rate. OBJECTIVES: This study was aimed to molecular detection and subtyping of AMPV infection in broiler flocks using RT-PCR method in Semnan province on samples from 2016 to2020. METHODS: Sampling was carried out from the upper part of the trachea, choana, and sinuses of broiler chickens from the 85 broiler flocks. All flocks were more than 3 weeks of age. In total 10 swabs were taken from each flock, while each 5 were pooled as one sample (total two samples per flock). The samples were transferred to the laboratory for RNA extraction and RT-PCR amplification. RESULTS: Out of 85 tested broiler flocks, 30 (35.3%) were positive for AMPV using the Nd/Nx primer set. In addition, 28 positive samples were found to be of subtype B using the Ga/G12 primer set and 2 remaining positive samples were non-subtype B, probably A, C or D subtypes. CONCLUSIONS: Since AMPV vaccination was not performed in Semnan province, it can be concluded that some cases were infected with the natural viruses. Therefore, vaccination could be effective in controlling AMPV-induced respiratory distress.https://ijvm.ut.ac.ir/article_81086_53465ca05e5001a208735c11b8ab420f.pdfavian metapneumovirusbroiler flocksnd/nx primerrt-pcrsubtype b |
| spellingShingle | Amir Darebaghi Seyed Hesamoddin Emadi Chashmi Khatereh Kafshdozan Hossein hossein Molecular Detection of Avian Metapneumovirus in Semnan BroilerFarms Iranian Journal of Veterinary Medicine avian metapneumovirus broiler flocks nd/nx primer rt-pcr subtype b |
| title | Molecular Detection of Avian Metapneumovirus in Semnan BroilerFarms |
| title_full | Molecular Detection of Avian Metapneumovirus in Semnan BroilerFarms |
| title_fullStr | Molecular Detection of Avian Metapneumovirus in Semnan BroilerFarms |
| title_full_unstemmed | Molecular Detection of Avian Metapneumovirus in Semnan BroilerFarms |
| title_short | Molecular Detection of Avian Metapneumovirus in Semnan BroilerFarms |
| title_sort | molecular detection of avian metapneumovirus in semnan broilerfarms |
| topic | avian metapneumovirus broiler flocks nd/nx primer rt-pcr subtype b |
| url | https://ijvm.ut.ac.ir/article_81086_53465ca05e5001a208735c11b8ab420f.pdf |
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