Cytotoxic Activity against A549 Human Lung Cancer Cells and ADMET Analysis of New Pyrazole Derivatives
Two new pyrazole derivatives, namely compound <b>1</b> and compound <b>2,</b> have been synthesized, and their biological activity has been evaluated. Monocrystals of the obtained compounds were thoroughly investigated using single-crystal X-ray diffraction analysis, FTIR spe...
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| Format: | Article |
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MDPI AG
2021-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/13/6692 |
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| author | Agnieszka Czylkowska Małgorzata Szczesio Anita Raducka Bartłomiej Rogalewicz Paweł Kręcisz Kamila Czarnecka Paweł Szymański Monika Pitucha Tomasz Pawlak |
| author_facet | Agnieszka Czylkowska Małgorzata Szczesio Anita Raducka Bartłomiej Rogalewicz Paweł Kręcisz Kamila Czarnecka Paweł Szymański Monika Pitucha Tomasz Pawlak |
| author_sort | Agnieszka Czylkowska |
| collection | DOAJ |
| description | Two new pyrazole derivatives, namely compound <b>1</b> and compound <b>2,</b> have been synthesized, and their biological activity has been evaluated. Monocrystals of the obtained compounds were thoroughly investigated using single-crystal X-ray diffraction analysis, FTIR spectroscopy, and NMR spectroscopy. The results gathered from all three techniques are in good agreement, provide complete information about the structures of <b>1</b> and <b>2</b>, and confirm their high purity. Thermal properties were studied using thermogravimetric analysis; both <b>1</b> and <b>2</b> are stable at room temperature. In order to better characterize <b>1</b> and <b>2</b>, some physicochemical and biological properties have been evaluated using ADMET analysis. The cytotoxic activity of both compounds was determined using the MTT assay on the A549 cell line in comparison with etoposide. It was determined that compound <b>2</b> was effective in the inhibition of human lung adenocarcinoma cell growth and may be a promising compound for the treatment of lung cancer. |
| first_indexed | 2024-03-10T10:10:12Z |
| format | Article |
| id | doaj.art-e54b0167553e4684a0d385973b0fcc3c |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T10:10:12Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e54b0167553e4684a0d385973b0fcc3c2023-11-22T01:15:05ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-06-012213669210.3390/ijms22136692Cytotoxic Activity against A549 Human Lung Cancer Cells and ADMET Analysis of New Pyrazole DerivativesAgnieszka Czylkowska0Małgorzata Szczesio1Anita Raducka2Bartłomiej Rogalewicz3Paweł Kręcisz4Kamila Czarnecka5Paweł Szymański6Monika Pitucha7Tomasz Pawlak8Institute of General and Ecological Chemistry, Faculty of Chemistry, Lodz University of Technology, 90-924 Lodz, PolandInstitute of General and Ecological Chemistry, Faculty of Chemistry, Lodz University of Technology, 90-924 Lodz, PolandInstitute of General and Ecological Chemistry, Faculty of Chemistry, Lodz University of Technology, 90-924 Lodz, PolandInstitute of General and Ecological Chemistry, Faculty of Chemistry, Lodz University of Technology, 90-924 Lodz, PolandDepartment of Pharmaceutical Chemistry, Drug Analyses and Radiopharmacy, Faculty of Pharmacy, Medical University of Lodz, 90-151 Lodz, PolandDepartment of Pharmaceutical Chemistry, Drug Analyses and Radiopharmacy, Faculty of Pharmacy, Medical University of Lodz, 90-151 Lodz, PolandDepartment of Pharmaceutical Chemistry, Drug Analyses and Radiopharmacy, Faculty of Pharmacy, Medical University of Lodz, 90-151 Lodz, PolandIndependent Radiopharmacy Unit, Faculty of Pharmacy, Medical University of Lublin, 20-093 Lublin, PolandCentre of Molecular and Macromolecular Studies, Polish Academy of Sciences, 90-363 Lodz, PolandTwo new pyrazole derivatives, namely compound <b>1</b> and compound <b>2,</b> have been synthesized, and their biological activity has been evaluated. Monocrystals of the obtained compounds were thoroughly investigated using single-crystal X-ray diffraction analysis, FTIR spectroscopy, and NMR spectroscopy. The results gathered from all three techniques are in good agreement, provide complete information about the structures of <b>1</b> and <b>2</b>, and confirm their high purity. Thermal properties were studied using thermogravimetric analysis; both <b>1</b> and <b>2</b> are stable at room temperature. In order to better characterize <b>1</b> and <b>2</b>, some physicochemical and biological properties have been evaluated using ADMET analysis. The cytotoxic activity of both compounds was determined using the MTT assay on the A549 cell line in comparison with etoposide. It was determined that compound <b>2</b> was effective in the inhibition of human lung adenocarcinoma cell growth and may be a promising compound for the treatment of lung cancer.https://www.mdpi.com/1422-0067/22/13/6692cytotoxic activitypyrazole derivativesMTT assayADMET analysissingle-crystal diffractionFTIR spectroscopy |
| spellingShingle | Agnieszka Czylkowska Małgorzata Szczesio Anita Raducka Bartłomiej Rogalewicz Paweł Kręcisz Kamila Czarnecka Paweł Szymański Monika Pitucha Tomasz Pawlak Cytotoxic Activity against A549 Human Lung Cancer Cells and ADMET Analysis of New Pyrazole Derivatives International Journal of Molecular Sciences cytotoxic activity pyrazole derivatives MTT assay ADMET analysis single-crystal diffraction FTIR spectroscopy |
| title | Cytotoxic Activity against A549 Human Lung Cancer Cells and ADMET Analysis of New Pyrazole Derivatives |
| title_full | Cytotoxic Activity against A549 Human Lung Cancer Cells and ADMET Analysis of New Pyrazole Derivatives |
| title_fullStr | Cytotoxic Activity against A549 Human Lung Cancer Cells and ADMET Analysis of New Pyrazole Derivatives |
| title_full_unstemmed | Cytotoxic Activity against A549 Human Lung Cancer Cells and ADMET Analysis of New Pyrazole Derivatives |
| title_short | Cytotoxic Activity against A549 Human Lung Cancer Cells and ADMET Analysis of New Pyrazole Derivatives |
| title_sort | cytotoxic activity against a549 human lung cancer cells and admet analysis of new pyrazole derivatives |
| topic | cytotoxic activity pyrazole derivatives MTT assay ADMET analysis single-crystal diffraction FTIR spectroscopy |
| url | https://www.mdpi.com/1422-0067/22/13/6692 |
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