In-Vitro Evaluation of Novel Polycaprolactone/ Chitosan/ Carbon Nano Tube Scaffold for Tissue Regeneration
Background: Many patients lose their organs or tissues due to disease, trauma, or a variety of genetic disorders. Tissue engineering is a multidisciplinary science to regenerate or restore tissue or organ function and an appropriate scaffold is the first and certainly a crucial step in tissue engine...
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2022-08-01
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| Series: | Journal of Biomedical Physics and Engineering |
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| Online Access: | https://jbpe.sums.ac.ir/article_48485_aa44e691826d240eacb5410a7785a3a3.pdf |
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| author | Reza Fekrazad Farbod Tondnevi Mohamad Mahdi Abolhasani |
| author_facet | Reza Fekrazad Farbod Tondnevi Mohamad Mahdi Abolhasani |
| author_sort | Reza Fekrazad |
| collection | DOAJ |
| description | Background: Many patients lose their organs or tissues due to disease, trauma, or a variety of genetic disorders. Tissue engineering is a multidisciplinary science to regenerate or restore tissue or organ function and an appropriate scaffold is the first and certainly a crucial step in tissue engineering strategies. Objective: The purpose of this study is to fabricate and evaluate the in-vitro response of porous nano Polycaprolactone (PCL)/ chitosan/ multi-wall carbon nanotube (MWCNTs) scaffold for tissue regeneration.Material and Methods: In this experimental research, a novel scaffold containing MWCNTs in polycaprolactone/chitosan nanofibrous scaffold was synthesized by electrospinning technique. Results: According to scanning electron microscopy SEM images, by increasing the number of MWCNT in the scaffold by 2%, the average diameter decreased significantly for fabricated scaffolds with 5% MWCNTs. Based on the results, the scaffolds plunged from submicron to nanoscale fibers at about 80 nm. In addition, by adding more MWCNT to the nanofibrous scaffold, the biodegradation rate was decreased by 32%. However, mechanical characterization demonstrates that the higher level of MWCNT increases young modulus by 96%, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay illustrated that MWCNTs could enhance bioactivity and cell- scaffold relationship in addition to alkaline phosphatase (ALP). Conclusion: MWCNT significantly improves the physical and mechanical properties of fabricated scaffolds and in-vitro assessment demonstrated that the prepared nanofibrous scaffold containing 4% MWCNT could be a very useful biocompatible material for tissue engineering. |
| first_indexed | 2024-04-14T03:05:26Z |
| format | Article |
| id | doaj.art-e54c39223fdf4a4dbdfa9ba90eb3f3f8 |
| institution | Directory Open Access Journal |
| issn | 2251-7200 |
| language | English |
| last_indexed | 2024-04-14T03:05:26Z |
| publishDate | 2022-08-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Journal of Biomedical Physics and Engineering |
| spelling | doaj.art-e54c39223fdf4a4dbdfa9ba90eb3f3f82022-12-22T02:15:45ZengShiraz University of Medical SciencesJournal of Biomedical Physics and Engineering2251-72002022-08-0112439540210.31661/jbpe.v0i0.118848485In-Vitro Evaluation of Novel Polycaprolactone/ Chitosan/ Carbon Nano Tube Scaffold for Tissue RegenerationReza Fekrazad0Farbod Tondnevi1Mohamad Mahdi Abolhasani2DDS, Department of Periodontology, Dental Faculty, AJA University of Medical Sciences, Tehran, IranPhD, Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, IranPhD, Department of Nanotechnology & Advanced Materials, Materials & Energy Research Center (MERC), Karaj, IranBackground: Many patients lose their organs or tissues due to disease, trauma, or a variety of genetic disorders. Tissue engineering is a multidisciplinary science to regenerate or restore tissue or organ function and an appropriate scaffold is the first and certainly a crucial step in tissue engineering strategies. Objective: The purpose of this study is to fabricate and evaluate the in-vitro response of porous nano Polycaprolactone (PCL)/ chitosan/ multi-wall carbon nanotube (MWCNTs) scaffold for tissue regeneration.Material and Methods: In this experimental research, a novel scaffold containing MWCNTs in polycaprolactone/chitosan nanofibrous scaffold was synthesized by electrospinning technique. Results: According to scanning electron microscopy SEM images, by increasing the number of MWCNT in the scaffold by 2%, the average diameter decreased significantly for fabricated scaffolds with 5% MWCNTs. Based on the results, the scaffolds plunged from submicron to nanoscale fibers at about 80 nm. In addition, by adding more MWCNT to the nanofibrous scaffold, the biodegradation rate was decreased by 32%. However, mechanical characterization demonstrates that the higher level of MWCNT increases young modulus by 96%, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay illustrated that MWCNTs could enhance bioactivity and cell- scaffold relationship in addition to alkaline phosphatase (ALP). Conclusion: MWCNT significantly improves the physical and mechanical properties of fabricated scaffolds and in-vitro assessment demonstrated that the prepared nanofibrous scaffold containing 4% MWCNT could be a very useful biocompatible material for tissue engineering.https://jbpe.sums.ac.ir/article_48485_aa44e691826d240eacb5410a7785a3a3.pdfmulti-wall carbon nano tubeelectrospun scaffoldnanofiberschitosanpolycaprolactonetissue engineering |
| spellingShingle | Reza Fekrazad Farbod Tondnevi Mohamad Mahdi Abolhasani In-Vitro Evaluation of Novel Polycaprolactone/ Chitosan/ Carbon Nano Tube Scaffold for Tissue Regeneration Journal of Biomedical Physics and Engineering multi-wall carbon nano tube electrospun scaffold nanofibers chitosan polycaprolactone tissue engineering |
| title | In-Vitro Evaluation of Novel Polycaprolactone/ Chitosan/ Carbon Nano Tube Scaffold for Tissue Regeneration |
| title_full | In-Vitro Evaluation of Novel Polycaprolactone/ Chitosan/ Carbon Nano Tube Scaffold for Tissue Regeneration |
| title_fullStr | In-Vitro Evaluation of Novel Polycaprolactone/ Chitosan/ Carbon Nano Tube Scaffold for Tissue Regeneration |
| title_full_unstemmed | In-Vitro Evaluation of Novel Polycaprolactone/ Chitosan/ Carbon Nano Tube Scaffold for Tissue Regeneration |
| title_short | In-Vitro Evaluation of Novel Polycaprolactone/ Chitosan/ Carbon Nano Tube Scaffold for Tissue Regeneration |
| title_sort | in vitro evaluation of novel polycaprolactone chitosan carbon nano tube scaffold for tissue regeneration |
| topic | multi-wall carbon nano tube electrospun scaffold nanofibers chitosan polycaprolactone tissue engineering |
| url | https://jbpe.sums.ac.ir/article_48485_aa44e691826d240eacb5410a7785a3a3.pdf |
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