Leishmania infantum induces high phagocytic capacity and intracellular nitric oxide production by human proinflammatory monocyte
BACKGROUND The mechanism of resistance to SbIII in Leishmania is complex, multifactorial and involves not only biochemical mechanisms, but also other elements, such as the immune system of the host. OBJECTIVES In this study, putative changes in the immunological profile of human monocytes infecte...
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| Format: | Article |
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Fundação Oswaldo Cruz (FIOCRUZ)
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/pdf/mioc/v115/1678-8060-mioc-115-e190408.pdf |
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| author | Christiana Vargas Ribeiro Bruna Fonte Boa Rocha Edward Oliveira Andrea Teixeira-Carvalho Olindo Assis Martins-Filho Silvane Maria Fonseca Murta Vanessa Peruhype-Magalhães |
| author_facet | Christiana Vargas Ribeiro Bruna Fonte Boa Rocha Edward Oliveira Andrea Teixeira-Carvalho Olindo Assis Martins-Filho Silvane Maria Fonseca Murta Vanessa Peruhype-Magalhães |
| author_sort | Christiana Vargas Ribeiro |
| collection | DOAJ |
| description | BACKGROUND The mechanism of resistance to SbIII in Leishmania is complex, multifactorial and involves not only biochemical mechanisms, but also other elements, such as the immune system of the host. OBJECTIVES In this study, putative changes in the immunological profile of human monocytes infected with wild-type (WT) and antimony (SbIII)-resistant Leishmania (Viannia) braziliensis and Leishmania (Leishmania) infantum lines were evaluated. METHODS Susceptibility assays WT and SbIII-resistant L. braziliensis and L. infantum were performed using lines THP-1 human monocytic lineage. Phagocytic capacity, cytokine profile, intracellular nitric oxide (NO) production and surface carbohydrate residues profile were performed in peripheral blood monocytes by flow cytometry. FINDINGS The phagocytic capacity and intracellular NO production by classical (CD14++CD16-) and proinflammatory (CD14++CD16+) monocytes were higher in the presence of L. infantum lines compared to L. braziliensis lines. The results also highlight proinflammatory monocytes as the cellular subpopulation of major relevance in a phagocytosis event and NO expression. It is important to note that L. infantum induced a proinflammatory cytokine profile characterised by higher levels of TNF-α in culture supernatant than L. braziliensis. Conversely, both Leishmania lines induce high levels of IL-6 in culture supernatant. Analysis of the expression profile of surface carbohydrates showed that L. braziliensis presents 4.3-fold higher expression of galactose(β1,4)N-acetylglucosamine than L. infantum line. Interestingly, the expression level of α-N-acetylgalactosamine residues was 2-fold lower in the SbIII-resistant L. braziliensis line than its counterpart WT line, indicating differences in surface glycoconjugates between these lines. MAIN CONCLUSIONS Our results showed that L. braziliensis and L. infantum induce different innate immune responses and a highly inflammatory profile, which is characteristic of infection by L. infantum, the species associated with visceral disease. |
| first_indexed | 2024-03-12T09:34:36Z |
| format | Article |
| id | doaj.art-e54c5da1e7f5417da72d219973c8d6db |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T09:34:36Z |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-e54c5da1e7f5417da72d219973c8d6db2023-09-02T13:50:31ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-806010.1590/0074-02760190408Leishmania infantum induces high phagocytic capacity and intracellular nitric oxide production by human proinflammatory monocyteChristiana Vargas RibeiroBruna Fonte Boa RochaEdward OliveiraAndrea Teixeira-CarvalhoOlindo Assis Martins-FilhoSilvane Maria Fonseca MurtaVanessa Peruhype-MagalhãesBACKGROUND The mechanism of resistance to SbIII in Leishmania is complex, multifactorial and involves not only biochemical mechanisms, but also other elements, such as the immune system of the host. OBJECTIVES In this study, putative changes in the immunological profile of human monocytes infected with wild-type (WT) and antimony (SbIII)-resistant Leishmania (Viannia) braziliensis and Leishmania (Leishmania) infantum lines were evaluated. METHODS Susceptibility assays WT and SbIII-resistant L. braziliensis and L. infantum were performed using lines THP-1 human monocytic lineage. Phagocytic capacity, cytokine profile, intracellular nitric oxide (NO) production and surface carbohydrate residues profile were performed in peripheral blood monocytes by flow cytometry. FINDINGS The phagocytic capacity and intracellular NO production by classical (CD14++CD16-) and proinflammatory (CD14++CD16+) monocytes were higher in the presence of L. infantum lines compared to L. braziliensis lines. The results also highlight proinflammatory monocytes as the cellular subpopulation of major relevance in a phagocytosis event and NO expression. It is important to note that L. infantum induced a proinflammatory cytokine profile characterised by higher levels of TNF-α in culture supernatant than L. braziliensis. Conversely, both Leishmania lines induce high levels of IL-6 in culture supernatant. Analysis of the expression profile of surface carbohydrates showed that L. braziliensis presents 4.3-fold higher expression of galactose(β1,4)N-acetylglucosamine than L. infantum line. Interestingly, the expression level of α-N-acetylgalactosamine residues was 2-fold lower in the SbIII-resistant L. braziliensis line than its counterpart WT line, indicating differences in surface glycoconjugates between these lines. MAIN CONCLUSIONS Our results showed that L. braziliensis and L. infantum induce different innate immune responses and a highly inflammatory profile, which is characteristic of infection by L. infantum, the species associated with visceral disease.http://www.scielo.br/pdf/mioc/v115/1678-8060-mioc-115-e190408.pdfL. braziliensisL. infantummonocyte subsetslectinscytokinesnitric oxidesurface glycoconjugates |
| spellingShingle | Christiana Vargas Ribeiro Bruna Fonte Boa Rocha Edward Oliveira Andrea Teixeira-Carvalho Olindo Assis Martins-Filho Silvane Maria Fonseca Murta Vanessa Peruhype-Magalhães Leishmania infantum induces high phagocytic capacity and intracellular nitric oxide production by human proinflammatory monocyte Memorias do Instituto Oswaldo Cruz L. braziliensis L. infantum monocyte subsets lectins cytokines nitric oxide surface glycoconjugates |
| title | Leishmania infantum induces high phagocytic capacity and intracellular nitric oxide production by human proinflammatory monocyte |
| title_full | Leishmania infantum induces high phagocytic capacity and intracellular nitric oxide production by human proinflammatory monocyte |
| title_fullStr | Leishmania infantum induces high phagocytic capacity and intracellular nitric oxide production by human proinflammatory monocyte |
| title_full_unstemmed | Leishmania infantum induces high phagocytic capacity and intracellular nitric oxide production by human proinflammatory monocyte |
| title_short | Leishmania infantum induces high phagocytic capacity and intracellular nitric oxide production by human proinflammatory monocyte |
| title_sort | leishmania infantum induces high phagocytic capacity and intracellular nitric oxide production by human proinflammatory monocyte |
| topic | L. braziliensis L. infantum monocyte subsets lectins cytokines nitric oxide surface glycoconjugates |
| url | http://www.scielo.br/pdf/mioc/v115/1678-8060-mioc-115-e190408.pdf |
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