Effects of Tributyrin Supplementation on Growth Performance, Insulin, Blood Metabolites and Gut Microbiota in Weaned Piglets
The aim of this study was to investigate the effects of tributyrin supplementation on the production traits, the main metabolic parameters and gut microbiota in weaned piglets. One hundred and twenty crossbred piglets (Large White × Landrace) were randomly divided into two experimental groups (six p...
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| Format: | Article |
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MDPI AG
2020-04-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/4/726 |
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| author | Stefania Sotira Matteo Dell’Anno Valentina Caprarulo Monika Hejna Federica Pirrone Maria Luisa Callegari Telma Vieira Tucci Luciana Rossi |
| author_facet | Stefania Sotira Matteo Dell’Anno Valentina Caprarulo Monika Hejna Federica Pirrone Maria Luisa Callegari Telma Vieira Tucci Luciana Rossi |
| author_sort | Stefania Sotira |
| collection | DOAJ |
| description | The aim of this study was to investigate the effects of tributyrin supplementation on the production traits, the main metabolic parameters and gut microbiota in weaned piglets. One hundred and twenty crossbred piglets (Large White × Landrace) were randomly divided into two experimental groups (six pens each; 10 piglets per pen): the control group (CTRL), that received a basal diet, and the tributyrin group (TRIB) that received the basal diet supplemented with 0.2% tributyrin. The experimental period lasted 40 days. Production traits were measured at days 14, 28 and 40. A subset composed of 48 animals (<i>n</i> = 4 for each pen; <i>n</i> = 24 per group) was considered for the evaluation of serum metabolic parameters and hair cortisol by enzyme-linked immunosorbent assay (ELISA), and faecal microbiota by real-time polymerase chain reaction (PCR). Our results showed that the treatment significantly increased body weight (BW) at day 28 and day 40 (<i>p</i> = 0.0279 and <i>p</i> = 0.0006, respectively) and average daily gain (ADG) from day 28 to day 40 (<i>p</i> = 0.046). Gain to feed ratio (G:F) was significantly higher throughout the experimental period (<i>p</i> = 0.049). Even if the serum parameters were in the physiological range, albumin, albumin/globulin (A/G) ratio, glucose and high-density lipoproteins (HDL) fraction were significantly higher in the TRIB group. On the contrary, tributyrin significantly decreased the urea blood concentration (<i>p</i> = 0.0026), which was correlated with lean gain and feed efficiency. Moreover, serum insulin concentration, which has a regulatory effect on protein and lipid metabolism, was significantly higher in the TRIB group (<i>p</i> = 0.0187). In conclusion, this study demonstrated that tributyrin can be considered as a valid feed additive for weaned piglets. |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T20:18:50Z |
| publishDate | 2020-04-01 |
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| spelling | doaj.art-e54c9ec6f3aa4c76b1f59054635e19f92023-11-19T22:21:44ZengMDPI AGAnimals2076-26152020-04-0110472610.3390/ani10040726Effects of Tributyrin Supplementation on Growth Performance, Insulin, Blood Metabolites and Gut Microbiota in Weaned PigletsStefania Sotira0Matteo Dell’Anno1Valentina Caprarulo2Monika Hejna3Federica Pirrone4Maria Luisa Callegari5Telma Vieira Tucci6Luciana Rossi7Department of Health, Animal Science and Food Safety, Università degli Studi di Milano, via Trentacoste 2, 20134 Milan, ItalyDepartment of Health, Animal Science and Food Safety, Università degli Studi di Milano, via Trentacoste 2, 20134 Milan, ItalyDepartment of Health, Animal Science and Food Safety, Università degli Studi di Milano, via Trentacoste 2, 20134 Milan, ItalyDepartment of Health, Animal Science and Food Safety, Università degli Studi di Milano, via Trentacoste 2, 20134 Milan, ItalyDepartment of Veterinary Medicine, Università degli Studi di Milano, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Sustainable Food Process, Faculty of Agriculture, Food and Environmental Sciences, Università Cattolica del Sacro Cuore, via Emilia Parmense 84, 29122 Piacenza, ItalyFreelance Veterinarian, Via Fratelli Bandiera 28, 46100 Mantova, ItalyDepartment of Health, Animal Science and Food Safety, Università degli Studi di Milano, via Trentacoste 2, 20134 Milan, ItalyThe aim of this study was to investigate the effects of tributyrin supplementation on the production traits, the main metabolic parameters and gut microbiota in weaned piglets. One hundred and twenty crossbred piglets (Large White × Landrace) were randomly divided into two experimental groups (six pens each; 10 piglets per pen): the control group (CTRL), that received a basal diet, and the tributyrin group (TRIB) that received the basal diet supplemented with 0.2% tributyrin. The experimental period lasted 40 days. Production traits were measured at days 14, 28 and 40. A subset composed of 48 animals (<i>n</i> = 4 for each pen; <i>n</i> = 24 per group) was considered for the evaluation of serum metabolic parameters and hair cortisol by enzyme-linked immunosorbent assay (ELISA), and faecal microbiota by real-time polymerase chain reaction (PCR). Our results showed that the treatment significantly increased body weight (BW) at day 28 and day 40 (<i>p</i> = 0.0279 and <i>p</i> = 0.0006, respectively) and average daily gain (ADG) from day 28 to day 40 (<i>p</i> = 0.046). Gain to feed ratio (G:F) was significantly higher throughout the experimental period (<i>p</i> = 0.049). Even if the serum parameters were in the physiological range, albumin, albumin/globulin (A/G) ratio, glucose and high-density lipoproteins (HDL) fraction were significantly higher in the TRIB group. On the contrary, tributyrin significantly decreased the urea blood concentration (<i>p</i> = 0.0026), which was correlated with lean gain and feed efficiency. Moreover, serum insulin concentration, which has a regulatory effect on protein and lipid metabolism, was significantly higher in the TRIB group (<i>p</i> = 0.0187). In conclusion, this study demonstrated that tributyrin can be considered as a valid feed additive for weaned piglets.https://www.mdpi.com/2076-2615/10/4/726pig nutritionantibiotic alternativesfeed additivesbutyratelactobacilli |
| spellingShingle | Stefania Sotira Matteo Dell’Anno Valentina Caprarulo Monika Hejna Federica Pirrone Maria Luisa Callegari Telma Vieira Tucci Luciana Rossi Effects of Tributyrin Supplementation on Growth Performance, Insulin, Blood Metabolites and Gut Microbiota in Weaned Piglets Animals pig nutrition antibiotic alternatives feed additives butyrate lactobacilli |
| title | Effects of Tributyrin Supplementation on Growth Performance, Insulin, Blood Metabolites and Gut Microbiota in Weaned Piglets |
| title_full | Effects of Tributyrin Supplementation on Growth Performance, Insulin, Blood Metabolites and Gut Microbiota in Weaned Piglets |
| title_fullStr | Effects of Tributyrin Supplementation on Growth Performance, Insulin, Blood Metabolites and Gut Microbiota in Weaned Piglets |
| title_full_unstemmed | Effects of Tributyrin Supplementation on Growth Performance, Insulin, Blood Metabolites and Gut Microbiota in Weaned Piglets |
| title_short | Effects of Tributyrin Supplementation on Growth Performance, Insulin, Blood Metabolites and Gut Microbiota in Weaned Piglets |
| title_sort | effects of tributyrin supplementation on growth performance insulin blood metabolites and gut microbiota in weaned piglets |
| topic | pig nutrition antibiotic alternatives feed additives butyrate lactobacilli |
| url | https://www.mdpi.com/2076-2615/10/4/726 |
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