A Lycopene ε-Cyclase TILLING Allele Enhances Lycopene and Carotenoid Content in Fruit and Improves Drought Stress Tolerance in Tomato Plants
In the scenario of climate change, the availability of genetic resources for tomato cultivation that combine improved nutritional properties and more tolerance to water deficiency is highly desirable. Within this context, the molecular screenings of the Red Setter cultivar-based TILLING platform led...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/14/6/1284 |
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| author | Angelo Petrozza Stephan Summerer Donato Melfi Teresa Mango Filippo Vurro Manuele Bettelli Michela Janni Francesco Cellini Filomena Carriero |
| author_facet | Angelo Petrozza Stephan Summerer Donato Melfi Teresa Mango Filippo Vurro Manuele Bettelli Michela Janni Francesco Cellini Filomena Carriero |
| author_sort | Angelo Petrozza |
| collection | DOAJ |
| description | In the scenario of climate change, the availability of genetic resources for tomato cultivation that combine improved nutritional properties and more tolerance to water deficiency is highly desirable. Within this context, the molecular screenings of the Red Setter cultivar-based TILLING platform led to the isolation of a novel lycopene ε-cyclase gene (SlLCY-E) variant (G/3378/T) that produces modifications in the carotenoid content of tomato leaves and fruits. In leaf tissue, the novel G/3378/T SlLCY-E allele enhances β,β-xanthophyll content at the expense of lutein, which decreases, while in ripe tomato fruit the TILLING mutation induces a significant increase in lycopene and total carotenoid content. Under drought stress conditions, the G/3378/T SlLCY-E plants produce more abscisic acid (ABA) and still conserve their leaf carotenoid profile (reduction of lutein and increase in β,β-xanthophyll content). Furthermore, under said conditions, the mutant plants grow much better and are more tolerant to drought stress, as revealed by digital-based image analysis and in vivo monitoring of the OECT (Organic Electrochemical Transistor) sensor. Altogether, our data indicate that the novel TILLING SlLCY-E allelic variant is a valuable genetic resource that can be used for developing new tomato varieties, improved in drought stress tolerance and enriched in fruit lycopene and carotenoid content. |
| first_indexed | 2024-03-11T02:25:52Z |
| format | Article |
| id | doaj.art-e55492d2c8774bacba419a1f189e6367 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-11T02:25:52Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-e55492d2c8774bacba419a1f189e63672023-11-18T10:35:21ZengMDPI AGGenes2073-44252023-06-01146128410.3390/genes14061284A Lycopene ε-Cyclase TILLING Allele Enhances Lycopene and Carotenoid Content in Fruit and Improves Drought Stress Tolerance in Tomato PlantsAngelo Petrozza0Stephan Summerer1Donato Melfi2Teresa Mango3Filippo Vurro4Manuele Bettelli5Michela Janni6Francesco Cellini7Filomena Carriero8ALSIA Centro Ricerche Metapontum Agrobios, s.s. Jonica 106, km 448.2, 75010 Metaponto, MT, ItalyALSIA Centro Ricerche Metapontum Agrobios, s.s. Jonica 106, km 448.2, 75010 Metaponto, MT, ItalyALSIA Centro Ricerche Metapontum Agrobios, s.s. Jonica 106, km 448.2, 75010 Metaponto, MT, ItalyALSIA Centro Ricerche Metapontum Agrobios, s.s. Jonica 106, km 448.2, 75010 Metaponto, MT, ItalyIstituto dei Materiali per l’Elettronica e il Magnetismo (IMEM-CNR), Parco Area delle Scienze 37/A, 43121 Parma, ItalyIstituto dei Materiali per l’Elettronica e il Magnetismo (IMEM-CNR), Parco Area delle Scienze 37/A, 43121 Parma, ItalyIstituto dei Materiali per l’Elettronica e il Magnetismo (IMEM-CNR), Parco Area delle Scienze 37/A, 43121 Parma, ItalyALSIA Centro Ricerche Metapontum Agrobios, s.s. Jonica 106, km 448.2, 75010 Metaponto, MT, ItalyALSIA Centro Ricerche Metapontum Agrobios, s.s. Jonica 106, km 448.2, 75010 Metaponto, MT, ItalyIn the scenario of climate change, the availability of genetic resources for tomato cultivation that combine improved nutritional properties and more tolerance to water deficiency is highly desirable. Within this context, the molecular screenings of the Red Setter cultivar-based TILLING platform led to the isolation of a novel lycopene ε-cyclase gene (SlLCY-E) variant (G/3378/T) that produces modifications in the carotenoid content of tomato leaves and fruits. In leaf tissue, the novel G/3378/T SlLCY-E allele enhances β,β-xanthophyll content at the expense of lutein, which decreases, while in ripe tomato fruit the TILLING mutation induces a significant increase in lycopene and total carotenoid content. Under drought stress conditions, the G/3378/T SlLCY-E plants produce more abscisic acid (ABA) and still conserve their leaf carotenoid profile (reduction of lutein and increase in β,β-xanthophyll content). Furthermore, under said conditions, the mutant plants grow much better and are more tolerant to drought stress, as revealed by digital-based image analysis and in vivo monitoring of the OECT (Organic Electrochemical Transistor) sensor. Altogether, our data indicate that the novel TILLING SlLCY-E allelic variant is a valuable genetic resource that can be used for developing new tomato varieties, improved in drought stress tolerance and enriched in fruit lycopene and carotenoid content.https://www.mdpi.com/2073-4425/14/6/1284<i>Solanum lycopersicum</i>induced mutationscarotenoid pathwayabiotic stressdigital image-based phenotypingbioristor |
| spellingShingle | Angelo Petrozza Stephan Summerer Donato Melfi Teresa Mango Filippo Vurro Manuele Bettelli Michela Janni Francesco Cellini Filomena Carriero A Lycopene ε-Cyclase TILLING Allele Enhances Lycopene and Carotenoid Content in Fruit and Improves Drought Stress Tolerance in Tomato Plants Genes <i>Solanum lycopersicum</i> induced mutations carotenoid pathway abiotic stress digital image-based phenotyping bioristor |
| title | A Lycopene ε-Cyclase TILLING Allele Enhances Lycopene and Carotenoid Content in Fruit and Improves Drought Stress Tolerance in Tomato Plants |
| title_full | A Lycopene ε-Cyclase TILLING Allele Enhances Lycopene and Carotenoid Content in Fruit and Improves Drought Stress Tolerance in Tomato Plants |
| title_fullStr | A Lycopene ε-Cyclase TILLING Allele Enhances Lycopene and Carotenoid Content in Fruit and Improves Drought Stress Tolerance in Tomato Plants |
| title_full_unstemmed | A Lycopene ε-Cyclase TILLING Allele Enhances Lycopene and Carotenoid Content in Fruit and Improves Drought Stress Tolerance in Tomato Plants |
| title_short | A Lycopene ε-Cyclase TILLING Allele Enhances Lycopene and Carotenoid Content in Fruit and Improves Drought Stress Tolerance in Tomato Plants |
| title_sort | lycopene ε cyclase tilling allele enhances lycopene and carotenoid content in fruit and improves drought stress tolerance in tomato plants |
| topic | <i>Solanum lycopersicum</i> induced mutations carotenoid pathway abiotic stress digital image-based phenotyping bioristor |
| url | https://www.mdpi.com/2073-4425/14/6/1284 |
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