Efficiency of transcription and translation of cell-free protein synthesis systems in cell-sized lipid vesicles with changing lipid composition determined by fluorescence measurements

Abstract To develop artificial cell models that mimic living cells, cell-sized lipid vesicles encapsulating cell-free protein synthesis (CFPS) systems are useful for protein expressions or artificial gene circuits for vesicle–vesicle communications. Therefore, investigating the transcriptional and t...

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Bibliographic Details
Main Authors: Akari Miwa, Masatoshi Wakamori, Tetsuro Ariyoshi, Yasushi Okada, Mikako Shirouzu, Takashi Umehara, Koki Kamiya
Format: Article
Language:English
Published: Nature Portfolio 2024-02-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-024-53135-8