Identification of unique cardiolipin and monolysocardiolipin species in Acinetobacter baumannii
Abstract Acidic glycerophospholipids play an important role in determining the resistance of Gram-negative bacteria to stress conditions and antibiotics. Acinetobacter baumannii, an opportunistic human pathogen which is responsible for an increasing number of nosocomial infections, exhibits broad an...
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| Format: | Article |
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Nature Portfolio
2017-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-03214-w |
| _version_ | 1818432441320210432 |
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| author | Patrizia Lopalco Julia Stahl Cosimo Annese Beate Averhoff Angela Corcelli |
| author_facet | Patrizia Lopalco Julia Stahl Cosimo Annese Beate Averhoff Angela Corcelli |
| author_sort | Patrizia Lopalco |
| collection | DOAJ |
| description | Abstract Acidic glycerophospholipids play an important role in determining the resistance of Gram-negative bacteria to stress conditions and antibiotics. Acinetobacter baumannii, an opportunistic human pathogen which is responsible for an increasing number of nosocomial infections, exhibits broad antibiotic resistances. Here lipids of A. baumannii have been analyzed by combined MALDI-TOF/MS and TLC analyses; in addition GC-MS analyses of fatty acid methyl esters released by methanolysis of membrane phospholipids have been performed. The main glycerophospholipids are phosphatidylethanolamine, phosphatidylglycerol, acyl-phosphatidylglycerol and cardiolipin together with monolysocardiolipin, a lysophospholipid only rarely detected in bacterial membranes. The major acyl chains in the phospholipids are C16:0 and C18:1, plus minor amounts of short chain fatty acids. The structures of the cardiolipin and monolysocardiolipin have been elucidated by post source decay mass spectrometry analysis. A large variety of cardiolipin and monolysocardiolipin species were found in A. baumannii. Similar lysocardiolipin levels were found in the two clinical strains A. baumannii ATCC19606T and AYE whereas in the nonpathogenic strain Acinetobacter baylyi ADP1 lysocardiolipin levels were highly reduced. |
| first_indexed | 2024-12-14T16:05:15Z |
| format | Article |
| id | doaj.art-e558bcf545f6431e9423b20fce194b04 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T16:05:15Z |
| publishDate | 2017-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e558bcf545f6431e9423b20fce194b042022-12-21T22:55:07ZengNature PortfolioScientific Reports2045-23222017-06-017111210.1038/s41598-017-03214-wIdentification of unique cardiolipin and monolysocardiolipin species in Acinetobacter baumanniiPatrizia Lopalco0Julia Stahl1Cosimo Annese2Beate Averhoff3Angela Corcelli4Department of Basic Medical Sciences, Neuroscience and Sense Organs, University of Bari Aldo MoroDepartment of Molecular Microbiology & Bioenergetics, Institute of Molecular Biosciences, Goethe-University Frankfurt am MainItalian National Council for Research - Institute for the Chemistry of OrganoMetallic Compounds (CNR-ICCOM)Department of Molecular Microbiology & Bioenergetics, Institute of Molecular Biosciences, Goethe-University Frankfurt am MainDepartment of Basic Medical Sciences, Neuroscience and Sense Organs, University of Bari Aldo MoroAbstract Acidic glycerophospholipids play an important role in determining the resistance of Gram-negative bacteria to stress conditions and antibiotics. Acinetobacter baumannii, an opportunistic human pathogen which is responsible for an increasing number of nosocomial infections, exhibits broad antibiotic resistances. Here lipids of A. baumannii have been analyzed by combined MALDI-TOF/MS and TLC analyses; in addition GC-MS analyses of fatty acid methyl esters released by methanolysis of membrane phospholipids have been performed. The main glycerophospholipids are phosphatidylethanolamine, phosphatidylglycerol, acyl-phosphatidylglycerol and cardiolipin together with monolysocardiolipin, a lysophospholipid only rarely detected in bacterial membranes. The major acyl chains in the phospholipids are C16:0 and C18:1, plus minor amounts of short chain fatty acids. The structures of the cardiolipin and monolysocardiolipin have been elucidated by post source decay mass spectrometry analysis. A large variety of cardiolipin and monolysocardiolipin species were found in A. baumannii. Similar lysocardiolipin levels were found in the two clinical strains A. baumannii ATCC19606T and AYE whereas in the nonpathogenic strain Acinetobacter baylyi ADP1 lysocardiolipin levels were highly reduced.https://doi.org/10.1038/s41598-017-03214-w |
| spellingShingle | Patrizia Lopalco Julia Stahl Cosimo Annese Beate Averhoff Angela Corcelli Identification of unique cardiolipin and monolysocardiolipin species in Acinetobacter baumannii Scientific Reports |
| title | Identification of unique cardiolipin and monolysocardiolipin species in Acinetobacter baumannii |
| title_full | Identification of unique cardiolipin and monolysocardiolipin species in Acinetobacter baumannii |
| title_fullStr | Identification of unique cardiolipin and monolysocardiolipin species in Acinetobacter baumannii |
| title_full_unstemmed | Identification of unique cardiolipin and monolysocardiolipin species in Acinetobacter baumannii |
| title_short | Identification of unique cardiolipin and monolysocardiolipin species in Acinetobacter baumannii |
| title_sort | identification of unique cardiolipin and monolysocardiolipin species in acinetobacter baumannii |
| url | https://doi.org/10.1038/s41598-017-03214-w |
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