Proteasome-mediated degradation antagonizes critical levels of the apoptosis-inducing C1D protein
<p>Abstract</p> <p>The C1D gene is expressed in a broad spectrum of mammalian cells and tissues but its product induces apoptotic cell death when exceeding a critical level. Critical levels are achieved in a fraction of cells by transient transfection with EGFP-tagged C1D expressio...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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BMC
2002-09-01
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| Series: | Cancer Cell International |
| Online Access: | http://www.cancerci.com/content/2/1/12 |
| _version_ | 1818753070347059200 |
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| author | Werner Dieter Stammer Hermann Rothbarth Karsten |
| author_facet | Werner Dieter Stammer Hermann Rothbarth Karsten |
| author_sort | Werner Dieter |
| collection | DOAJ |
| description | <p>Abstract</p> <p>The C1D gene is expressed in a broad spectrum of mammalian cells and tissues but its product induces apoptotic cell death when exceeding a critical level. Critical levels are achieved in a fraction of cells by transient transfection with EGFP-tagged C1D expression constructs. However, transfected cells expressing sub-critical levels of C1D(EGFP) escape apoptotic cell death by activation of a proteasome-mediated rescue mechanism. Inhibition of the proteasome-dependent degradation of the C1D(EGFP) protein results in a parallel increase of the intracellular C1D level and in the fraction of apoptotic cells.</p> |
| first_indexed | 2024-12-18T05:01:31Z |
| format | Article |
| id | doaj.art-e55919aae3ce40ab91d7e3e28156b833 |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-12-18T05:01:31Z |
| publishDate | 2002-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-e55919aae3ce40ab91d7e3e28156b8332022-12-21T21:20:08ZengBMCCancer Cell International1475-28672002-09-01211210.1186/1475-2867-2-12Proteasome-mediated degradation antagonizes critical levels of the apoptosis-inducing C1D proteinWerner DieterStammer HermannRothbarth Karsten<p>Abstract</p> <p>The C1D gene is expressed in a broad spectrum of mammalian cells and tissues but its product induces apoptotic cell death when exceeding a critical level. Critical levels are achieved in a fraction of cells by transient transfection with EGFP-tagged C1D expression constructs. However, transfected cells expressing sub-critical levels of C1D(EGFP) escape apoptotic cell death by activation of a proteasome-mediated rescue mechanism. Inhibition of the proteasome-dependent degradation of the C1D(EGFP) protein results in a parallel increase of the intracellular C1D level and in the fraction of apoptotic cells.</p>http://www.cancerci.com/content/2/1/12 |
| spellingShingle | Werner Dieter Stammer Hermann Rothbarth Karsten Proteasome-mediated degradation antagonizes critical levels of the apoptosis-inducing C1D protein Cancer Cell International |
| title | Proteasome-mediated degradation antagonizes critical levels of the apoptosis-inducing C1D protein |
| title_full | Proteasome-mediated degradation antagonizes critical levels of the apoptosis-inducing C1D protein |
| title_fullStr | Proteasome-mediated degradation antagonizes critical levels of the apoptosis-inducing C1D protein |
| title_full_unstemmed | Proteasome-mediated degradation antagonizes critical levels of the apoptosis-inducing C1D protein |
| title_short | Proteasome-mediated degradation antagonizes critical levels of the apoptosis-inducing C1D protein |
| title_sort | proteasome mediated degradation antagonizes critical levels of the apoptosis inducing c1d protein |
| url | http://www.cancerci.com/content/2/1/12 |
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