Beneficial Plant Microorganisms Affect the Endophytic Bacterial Communities of Durum Wheat Roots as Detected by Different Molecular Approaches
This study aimed at characterising the endophytic bacterial communities living in durum wheat roots, as affected by wheat cultivar and inoculation of the Arbuscular mycorrhizal fungus Funneliformis mosseae IMA1 and the wheat root endophytic bacterium Lactobacillus plantarum B.MD.R.A2. These microorg...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.02500/full |
| _version_ | 1818309493770944512 |
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| author | Monica Agnolucci Michela Palla Caterina Cristani Noemi Cavallo Manuela Giovannetti Maria De Angelis Marco Gobbetti Fabio Minervini |
| author_facet | Monica Agnolucci Michela Palla Caterina Cristani Noemi Cavallo Manuela Giovannetti Maria De Angelis Marco Gobbetti Fabio Minervini |
| author_sort | Monica Agnolucci |
| collection | DOAJ |
| description | This study aimed at characterising the endophytic bacterial communities living in durum wheat roots, as affected by wheat cultivar and inoculation of the Arbuscular mycorrhizal fungus Funneliformis mosseae IMA1 and the wheat root endophytic bacterium Lactobacillus plantarum B.MD.R.A2. These microorganisms were inoculated, alone or in combination, in durum wheat (cultivars Odisseo and Saragolla). Non-inoculated plants of each cultivar represented the controls. Forty-three days after sowing, roots were deprived of the epiphytic microbiota and subjected to DNA extraction. The DNA was used as template in PCR-DGGE analysis of the 16S rRNA gene (variable region V3–V5) and 16S (region V1–V3) metagenetics. Odisseo and Saragolla root endophytic bacterial biotas differed for number of OTUs and composition. In detail, Pseudomonas was higher in Odisseo than in Saragolla. The inoculation of F. mosseae and L. plantarum increased the abundance of Pseudomonas, some Actinobacteria (e.g., Streptomyces, Microbacterium, two genera including several plant growth promoting (PGP) strains) and Bacteroidetes in both cultivars. However, the endophytic bacterial biota of Saragolla roots inoculated just with lactobacilli did not differ from that of the control. The inoculation of Saragolla with F. mosseae, alone or in combination with lactobacilli, led to higher abundance of Rhodococcus, belonging to Actinobacteria and encompassing PGP strains. First, this work showed that F. mosseae and L. plantarum shape the endophytic bacterial biota of durum wheat roots. Abundance of some OTUs was affected by the microbial inoculation, depending on the cultivar. This result represents a starting point for exploitation of beneficial endophytes of wheat roots. |
| first_indexed | 2024-12-13T07:31:03Z |
| format | Article |
| id | doaj.art-e5596a6db909465f9b0f83ef6476f614 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T07:31:03Z |
| publishDate | 2019-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-e5596a6db909465f9b0f83ef6476f6142022-12-21T23:55:13ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-10-011010.3389/fmicb.2019.02500482306Beneficial Plant Microorganisms Affect the Endophytic Bacterial Communities of Durum Wheat Roots as Detected by Different Molecular ApproachesMonica Agnolucci0Michela Palla1Caterina Cristani2Noemi Cavallo3Manuela Giovannetti4Maria De Angelis5Marco Gobbetti6Fabio Minervini7Department of Agriculture, Food and Environment, University of Pisa, Pisa, ItalyDepartment of Agriculture, Food and Environment, University of Pisa, Pisa, ItalyDepartment of Agriculture, Food and Environment, University of Pisa, Pisa, ItalyDepartment of Soil, Plant and Food Science, University of Bari Aldo Moro, Bari, ItalyDepartment of Agriculture, Food and Environment, University of Pisa, Pisa, ItalyDepartment of Soil, Plant and Food Science, University of Bari Aldo Moro, Bari, ItalyFaculty of Science and Technology, Free University of Bozen-Bolzano, Bolzano, ItalyDepartment of Soil, Plant and Food Science, University of Bari Aldo Moro, Bari, ItalyThis study aimed at characterising the endophytic bacterial communities living in durum wheat roots, as affected by wheat cultivar and inoculation of the Arbuscular mycorrhizal fungus Funneliformis mosseae IMA1 and the wheat root endophytic bacterium Lactobacillus plantarum B.MD.R.A2. These microorganisms were inoculated, alone or in combination, in durum wheat (cultivars Odisseo and Saragolla). Non-inoculated plants of each cultivar represented the controls. Forty-three days after sowing, roots were deprived of the epiphytic microbiota and subjected to DNA extraction. The DNA was used as template in PCR-DGGE analysis of the 16S rRNA gene (variable region V3–V5) and 16S (region V1–V3) metagenetics. Odisseo and Saragolla root endophytic bacterial biotas differed for number of OTUs and composition. In detail, Pseudomonas was higher in Odisseo than in Saragolla. The inoculation of F. mosseae and L. plantarum increased the abundance of Pseudomonas, some Actinobacteria (e.g., Streptomyces, Microbacterium, two genera including several plant growth promoting (PGP) strains) and Bacteroidetes in both cultivars. However, the endophytic bacterial biota of Saragolla roots inoculated just with lactobacilli did not differ from that of the control. The inoculation of Saragolla with F. mosseae, alone or in combination with lactobacilli, led to higher abundance of Rhodococcus, belonging to Actinobacteria and encompassing PGP strains. First, this work showed that F. mosseae and L. plantarum shape the endophytic bacterial biota of durum wheat roots. Abundance of some OTUs was affected by the microbial inoculation, depending on the cultivar. This result represents a starting point for exploitation of beneficial endophytes of wheat roots.https://www.frontiersin.org/article/10.3389/fmicb.2019.02500/fullwheatArbuscular mycorrhizal symbiontsFunneliformis mosseaeLactobacillus plantarumroot endophytic bacterial biota |
| spellingShingle | Monica Agnolucci Michela Palla Caterina Cristani Noemi Cavallo Manuela Giovannetti Maria De Angelis Marco Gobbetti Fabio Minervini Beneficial Plant Microorganisms Affect the Endophytic Bacterial Communities of Durum Wheat Roots as Detected by Different Molecular Approaches Frontiers in Microbiology wheat Arbuscular mycorrhizal symbionts Funneliformis mosseae Lactobacillus plantarum root endophytic bacterial biota |
| title | Beneficial Plant Microorganisms Affect the Endophytic Bacterial Communities of Durum Wheat Roots as Detected by Different Molecular Approaches |
| title_full | Beneficial Plant Microorganisms Affect the Endophytic Bacterial Communities of Durum Wheat Roots as Detected by Different Molecular Approaches |
| title_fullStr | Beneficial Plant Microorganisms Affect the Endophytic Bacterial Communities of Durum Wheat Roots as Detected by Different Molecular Approaches |
| title_full_unstemmed | Beneficial Plant Microorganisms Affect the Endophytic Bacterial Communities of Durum Wheat Roots as Detected by Different Molecular Approaches |
| title_short | Beneficial Plant Microorganisms Affect the Endophytic Bacterial Communities of Durum Wheat Roots as Detected by Different Molecular Approaches |
| title_sort | beneficial plant microorganisms affect the endophytic bacterial communities of durum wheat roots as detected by different molecular approaches |
| topic | wheat Arbuscular mycorrhizal symbionts Funneliformis mosseae Lactobacillus plantarum root endophytic bacterial biota |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.02500/full |
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