Establishing a Genetic Detection Protocol of Single Nucleotide Polymorphisms Panels in Inbred Rats Based on Multiplex PCR-LDR

Establishing a Genetic Detection Protocol of Single Nucleotide Polymorphisms Panels in Inbred Rats Based on Multiplex PCR-LDR

Objective To establish a set of single nucleotide polymorphisms (SNP) detection protocol for inbred rats based on multiplex PCR-ligase detection reaction (LDR).Methods A total of 40 rats SNP sites were selected on chromosomes 1-20 and X of rats among 5 inbred strains of rats, and the 40 SNP sites we...

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Bibliographic Details
Main Authors: ZHAO Liya, NI Liju, ZHANG Caiqin, TANG Jianping, YAO Yangzheng, NIE Yanyan, GU Xiaoxue, ZHAO Ying
Format: Article
Language:zho
Published: Editorial Office of Laboratory Animal and Comparative Medicine 2023-10-01
Series:Shiyan dongwu yu bijiao yixue
Subjects:
inbred rats
single nucleotide polymorphisms
multiplex pcr-ldr
genetic detection
verification
Online Access:https://www.slarc.org.cn/dwyx/CN/abstract/abstract5098.shtml

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