<i>Kineococcus vitellinus</i> sp. nov., <i>Kineococcus indalonis</i> sp. nov. and <i>Kineococcus siccus</i> sp. nov., Isolated Nearby the Tabernas Desert (Almería, Spain)
Three novel Gram-positive, aerobic, chemoheterotrophic, motile, non-endospore-forming, orange-pigmented bacteria designated strains T13<sup>T</sup>, T90<sup>T</sup> and R8<sup>T</sup> were isolated from the Tabernas Desert biocrust (Almería, Spain). Cells of the t...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-10-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/8/10/1547 |
| _version_ | 1797551661592346624 |
|---|---|
| author | Esther Molina-Menor Helena Gimeno-Valero Javier Pascual Juli Peretó Manuel Porcar |
| author_facet | Esther Molina-Menor Helena Gimeno-Valero Javier Pascual Juli Peretó Manuel Porcar |
| author_sort | Esther Molina-Menor |
| collection | DOAJ |
| description | Three novel Gram-positive, aerobic, chemoheterotrophic, motile, non-endospore-forming, orange-pigmented bacteria designated strains T13<sup>T</sup>, T90<sup>T</sup> and R8<sup>T</sup> were isolated from the Tabernas Desert biocrust (Almería, Spain). Cells of the three strains were coccus-shaped and occurred singly, in pairs or clusters. The three strains were oxidase-negative and catalase-positive, and showed a mesophilic, neutrophilic and non-halophilic metabolism. Based on the 16S rRNA gene sequences, the closest neighbours of strains T13<sup>T</sup>, T90<sup>T</sup> and R8<sup>T</sup> were <i>Kineococcus aurantiacus</i> IFO 15268<sup>T</sup>, <i>Kineococcus gypseus</i> YIM 121300<sup>T</sup> and <i>Kineococcus radiotolerans</i> SRS 30216<sup>T</sup> (98.5%, 97.1% and 97.9% gene sequence similarity, respectively). The genomes were sequenced, and have been deposited in the GenBank/EMBL/DDBJ databases under the accession numbers JAAALL000000000, JAAALM000000000 and JAAALN000000000, respectively, for strains T13<sup>T</sup>, T90<sup>T</sup> and R8<sup>T</sup>. The average nucleotide identity (ANIb) and digital DNA-DNA hybridization (dDDH) values confirmed their adscription to three new species within the genus <i>Kineococcus</i>. The genomic G + C content of strains T13<sup>T</sup>, T90<sup>T</sup> and R8<sup>T</sup> ranged from 75.1% to 76.3%. The predominant fatty acid of all three strains was anteiso-C<sub>15:0</sub>. According to a polyphasic study, strains T13<sup>T</sup>, T90<sup>T</sup> and R8<sup>T</sup> are representatives of three new species in the genus <i>Kineococcus</i>, for which names <i>Kineococcus vitellinus</i> sp. nov. (type strain T13<sup>T</sup> = CECT 9936<sup>T</sup> = DSM 110024<sup>T</sup>), <i>Kineococcus indalonis</i> sp. nov. (type strain T90<sup>T</sup> = CECT 9938<sup>T</sup> = DSM 110026<sup>T</sup>) and <i>Kineococcus siccus</i> sp. nov. (type strain R8<sup>T</sup> = CECT 9937<sup>T</sup> = DSM 110025<sup>T</sup>) are proposed. |
| first_indexed | 2024-03-10T15:48:16Z |
| format | Article |
| id | doaj.art-e55fc9c173f749c5a4c3df5f479fd2af |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T15:48:16Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-e55fc9c173f749c5a4c3df5f479fd2af2023-11-20T16:16:41ZengMDPI AGMicroorganisms2076-26072020-10-01810154710.3390/microorganisms8101547<i>Kineococcus vitellinus</i> sp. nov., <i>Kineococcus indalonis</i> sp. nov. and <i>Kineococcus siccus</i> sp. nov., Isolated Nearby the Tabernas Desert (Almería, Spain)Esther Molina-Menor0Helena Gimeno-Valero1Javier Pascual2Juli Peretó3Manuel Porcar4Institute for Integrative Systems Biology I2SysBio (Universitat de València-CSIC), Calle del Catedràtic Agustin Escardino Benlloch 9, 46980 Paterna, SpainDarwin Bioprospecting Excellence SL. Parc Científic Universitat de València, Calle del Catedràtic Agustin Escardino Benlloch 9, 46980 Paterna, SpainDarwin Bioprospecting Excellence SL. Parc Científic Universitat de València, Calle del Catedràtic Agustin Escardino Benlloch 9, 46980 Paterna, SpainInstitute for Integrative Systems Biology I2SysBio (Universitat de València-CSIC), Calle del Catedràtic Agustin Escardino Benlloch 9, 46980 Paterna, SpainInstitute for Integrative Systems Biology I2SysBio (Universitat de València-CSIC), Calle del Catedràtic Agustin Escardino Benlloch 9, 46980 Paterna, SpainThree novel Gram-positive, aerobic, chemoheterotrophic, motile, non-endospore-forming, orange-pigmented bacteria designated strains T13<sup>T</sup>, T90<sup>T</sup> and R8<sup>T</sup> were isolated from the Tabernas Desert biocrust (Almería, Spain). Cells of the three strains were coccus-shaped and occurred singly, in pairs or clusters. The three strains were oxidase-negative and catalase-positive, and showed a mesophilic, neutrophilic and non-halophilic metabolism. Based on the 16S rRNA gene sequences, the closest neighbours of strains T13<sup>T</sup>, T90<sup>T</sup> and R8<sup>T</sup> were <i>Kineococcus aurantiacus</i> IFO 15268<sup>T</sup>, <i>Kineococcus gypseus</i> YIM 121300<sup>T</sup> and <i>Kineococcus radiotolerans</i> SRS 30216<sup>T</sup> (98.5%, 97.1% and 97.9% gene sequence similarity, respectively). The genomes were sequenced, and have been deposited in the GenBank/EMBL/DDBJ databases under the accession numbers JAAALL000000000, JAAALM000000000 and JAAALN000000000, respectively, for strains T13<sup>T</sup>, T90<sup>T</sup> and R8<sup>T</sup>. The average nucleotide identity (ANIb) and digital DNA-DNA hybridization (dDDH) values confirmed their adscription to three new species within the genus <i>Kineococcus</i>. The genomic G + C content of strains T13<sup>T</sup>, T90<sup>T</sup> and R8<sup>T</sup> ranged from 75.1% to 76.3%. The predominant fatty acid of all three strains was anteiso-C<sub>15:0</sub>. According to a polyphasic study, strains T13<sup>T</sup>, T90<sup>T</sup> and R8<sup>T</sup> are representatives of three new species in the genus <i>Kineococcus</i>, for which names <i>Kineococcus vitellinus</i> sp. nov. (type strain T13<sup>T</sup> = CECT 9936<sup>T</sup> = DSM 110024<sup>T</sup>), <i>Kineococcus indalonis</i> sp. nov. (type strain T90<sup>T</sup> = CECT 9938<sup>T</sup> = DSM 110026<sup>T</sup>) and <i>Kineococcus siccus</i> sp. nov. (type strain R8<sup>T</sup> = CECT 9937<sup>T</sup> = DSM 110025<sup>T</sup>) are proposed.https://www.mdpi.com/2076-2607/8/10/1547new taxabiocrustKineococcus indalonisKineococcus siccusKineococcus vitellinusTabernas Desert |
| spellingShingle | Esther Molina-Menor Helena Gimeno-Valero Javier Pascual Juli Peretó Manuel Porcar <i>Kineococcus vitellinus</i> sp. nov., <i>Kineococcus indalonis</i> sp. nov. and <i>Kineococcus siccus</i> sp. nov., Isolated Nearby the Tabernas Desert (Almería, Spain) Microorganisms new taxa biocrust Kineococcus indalonis Kineococcus siccus Kineococcus vitellinus Tabernas Desert |
| title | <i>Kineococcus vitellinus</i> sp. nov., <i>Kineococcus indalonis</i> sp. nov. and <i>Kineococcus siccus</i> sp. nov., Isolated Nearby the Tabernas Desert (Almería, Spain) |
| title_full | <i>Kineococcus vitellinus</i> sp. nov., <i>Kineococcus indalonis</i> sp. nov. and <i>Kineococcus siccus</i> sp. nov., Isolated Nearby the Tabernas Desert (Almería, Spain) |
| title_fullStr | <i>Kineococcus vitellinus</i> sp. nov., <i>Kineococcus indalonis</i> sp. nov. and <i>Kineococcus siccus</i> sp. nov., Isolated Nearby the Tabernas Desert (Almería, Spain) |
| title_full_unstemmed | <i>Kineococcus vitellinus</i> sp. nov., <i>Kineococcus indalonis</i> sp. nov. and <i>Kineococcus siccus</i> sp. nov., Isolated Nearby the Tabernas Desert (Almería, Spain) |
| title_short | <i>Kineococcus vitellinus</i> sp. nov., <i>Kineococcus indalonis</i> sp. nov. and <i>Kineococcus siccus</i> sp. nov., Isolated Nearby the Tabernas Desert (Almería, Spain) |
| title_sort | i kineococcus vitellinus i sp nov i kineococcus indalonis i sp nov and i kineococcus siccus i sp nov isolated nearby the tabernas desert almeria spain |
| topic | new taxa biocrust Kineococcus indalonis Kineococcus siccus Kineococcus vitellinus Tabernas Desert |
| url | https://www.mdpi.com/2076-2607/8/10/1547 |
| work_keys_str_mv | AT esthermolinamenor ikineococcusvitellinusispnovikineococcusindalonisispnovandikineococcussiccusispnovisolatednearbythetabernasdesertalmeriaspain AT helenagimenovalero ikineococcusvitellinusispnovikineococcusindalonisispnovandikineococcussiccusispnovisolatednearbythetabernasdesertalmeriaspain AT javierpascual ikineococcusvitellinusispnovikineococcusindalonisispnovandikineococcussiccusispnovisolatednearbythetabernasdesertalmeriaspain AT julipereto ikineococcusvitellinusispnovikineococcusindalonisispnovandikineococcussiccusispnovisolatednearbythetabernasdesertalmeriaspain AT manuelporcar ikineococcusvitellinusispnovikineococcusindalonisispnovandikineococcussiccusispnovisolatednearbythetabernasdesertalmeriaspain |