Phylogenetic relationships of the nematode subfamily Phascolostrongylinae from macropodid and vombatid marsupials inferred using mitochondrial protein sequence data

Abstract Background The subfamily Phascolostrongylinae (Superfamily Strongyloidea) comprises nematodes that are parasitic in the gastrointestinal tracts of macropodid (Family Macropodidae) and vombatid (Family Vombatidae) marsupials. Currently, nine genera and 20 species have been attributed to the...

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Main Authors: Tanapan Sukee, Ian Beveridge, Anson V. Koehler, Ross Hall, Robin B. Gasser, Abdul Jabbar
Format: Article
Language:English
Published: BMC 2021-10-01
Series:Parasites & Vectors
Subjects:
Online Access:https://doi.org/10.1186/s13071-021-05028-2
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author Tanapan Sukee
Ian Beveridge
Anson V. Koehler
Ross Hall
Robin B. Gasser
Abdul Jabbar
author_facet Tanapan Sukee
Ian Beveridge
Anson V. Koehler
Ross Hall
Robin B. Gasser
Abdul Jabbar
author_sort Tanapan Sukee
collection DOAJ
description Abstract Background The subfamily Phascolostrongylinae (Superfamily Strongyloidea) comprises nematodes that are parasitic in the gastrointestinal tracts of macropodid (Family Macropodidae) and vombatid (Family Vombatidae) marsupials. Currently, nine genera and 20 species have been attributed to the subfamily Phascolostrongylinae. Previous studies using sequence data sets for the internal transcribed spacers (ITS) of nuclear ribosomal DNA showed conflicting topologies between the Phascolostrongylinae and related subfamilies. Therefore, the aim of this study was to validate the phylogenetic relationships within the Phascolostrongylinae and its relationship with the families Chabertiidae and Strongylidae using mitochondrial amino acid sequences. Methods The sequences of all 12 mitochondrial protein-coding genes were obtained by next-generation sequencing of individual adult nematodes (n = 8) representing members of the Phascolostrongylinae. These sequences were conceptually translated and the phylogenetic relationships within the Phascolostrongylinae and its relationship with the families Chabertiidae and Strongylidae were inferred from aligned, concatenated amino acid sequence data sets. Results Within the Phascolostrongylinae, the wombat-specific genera grouped separately from the genera occurring in macropods. Two of the phascolostrongyline tribes were monophyletic, including Phascolostrongylinea and Hypodontinea, whereas the tribe Macropostrongyloidinea was paraphyletic. The tribe Phascolostrongylinea occurring in wombats was closely related to Oesophagostomum spp., also from the family Chabertiidae, which formed a sister relationship with the Phascolostrongylinae. Conclusion The current phylogenetic relationship within the subfamily Phascolostrongylinae supports findings from a previous study based on ITS sequence data. This study contributes also to the understanding of the phylogenetic position of the subfamily Phascolostrongylinae within the Chabertiidae. Future studies investigating the relationships between the Phascolostrongylinae and Cloacininae from macropodid marsupials may advance our knowledge of the phylogeny of strongyloid nematodes in marsupials. Graphical Abstract
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spelling doaj.art-e56026cf300749ada55463a74c6682812022-12-21T18:32:34ZengBMCParasites & Vectors1756-33052021-10-0114111110.1186/s13071-021-05028-2Phylogenetic relationships of the nematode subfamily Phascolostrongylinae from macropodid and vombatid marsupials inferred using mitochondrial protein sequence dataTanapan Sukee0Ian Beveridge1Anson V. Koehler2Ross Hall3Robin B. Gasser4Abdul Jabbar5Department of Veterinary Biosciences, Melbourne Veterinary School, The University of MelbourneDepartment of Veterinary Biosciences, Melbourne Veterinary School, The University of MelbourneDepartment of Veterinary Biosciences, Melbourne Veterinary School, The University of MelbourneDepartment of Veterinary Biosciences, Melbourne Veterinary School, The University of MelbourneDepartment of Veterinary Biosciences, Melbourne Veterinary School, The University of MelbourneDepartment of Veterinary Biosciences, Melbourne Veterinary School, The University of MelbourneAbstract Background The subfamily Phascolostrongylinae (Superfamily Strongyloidea) comprises nematodes that are parasitic in the gastrointestinal tracts of macropodid (Family Macropodidae) and vombatid (Family Vombatidae) marsupials. Currently, nine genera and 20 species have been attributed to the subfamily Phascolostrongylinae. Previous studies using sequence data sets for the internal transcribed spacers (ITS) of nuclear ribosomal DNA showed conflicting topologies between the Phascolostrongylinae and related subfamilies. Therefore, the aim of this study was to validate the phylogenetic relationships within the Phascolostrongylinae and its relationship with the families Chabertiidae and Strongylidae using mitochondrial amino acid sequences. Methods The sequences of all 12 mitochondrial protein-coding genes were obtained by next-generation sequencing of individual adult nematodes (n = 8) representing members of the Phascolostrongylinae. These sequences were conceptually translated and the phylogenetic relationships within the Phascolostrongylinae and its relationship with the families Chabertiidae and Strongylidae were inferred from aligned, concatenated amino acid sequence data sets. Results Within the Phascolostrongylinae, the wombat-specific genera grouped separately from the genera occurring in macropods. Two of the phascolostrongyline tribes were monophyletic, including Phascolostrongylinea and Hypodontinea, whereas the tribe Macropostrongyloidinea was paraphyletic. The tribe Phascolostrongylinea occurring in wombats was closely related to Oesophagostomum spp., also from the family Chabertiidae, which formed a sister relationship with the Phascolostrongylinae. Conclusion The current phylogenetic relationship within the subfamily Phascolostrongylinae supports findings from a previous study based on ITS sequence data. This study contributes also to the understanding of the phylogenetic position of the subfamily Phascolostrongylinae within the Chabertiidae. Future studies investigating the relationships between the Phascolostrongylinae and Cloacininae from macropodid marsupials may advance our knowledge of the phylogeny of strongyloid nematodes in marsupials. Graphical Abstracthttps://doi.org/10.1186/s13071-021-05028-2PhascolostrongylinaeMacropodid marsupialsVombatid marsupialsMitochondrial DNA
spellingShingle Tanapan Sukee
Ian Beveridge
Anson V. Koehler
Ross Hall
Robin B. Gasser
Abdul Jabbar
Phylogenetic relationships of the nematode subfamily Phascolostrongylinae from macropodid and vombatid marsupials inferred using mitochondrial protein sequence data
Parasites & Vectors
Phascolostrongylinae
Macropodid marsupials
Vombatid marsupials
Mitochondrial DNA
title Phylogenetic relationships of the nematode subfamily Phascolostrongylinae from macropodid and vombatid marsupials inferred using mitochondrial protein sequence data
title_full Phylogenetic relationships of the nematode subfamily Phascolostrongylinae from macropodid and vombatid marsupials inferred using mitochondrial protein sequence data
title_fullStr Phylogenetic relationships of the nematode subfamily Phascolostrongylinae from macropodid and vombatid marsupials inferred using mitochondrial protein sequence data
title_full_unstemmed Phylogenetic relationships of the nematode subfamily Phascolostrongylinae from macropodid and vombatid marsupials inferred using mitochondrial protein sequence data
title_short Phylogenetic relationships of the nematode subfamily Phascolostrongylinae from macropodid and vombatid marsupials inferred using mitochondrial protein sequence data
title_sort phylogenetic relationships of the nematode subfamily phascolostrongylinae from macropodid and vombatid marsupials inferred using mitochondrial protein sequence data
topic Phascolostrongylinae
Macropodid marsupials
Vombatid marsupials
Mitochondrial DNA
url https://doi.org/10.1186/s13071-021-05028-2
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