Identification and characterization of six novel tetraspanins from <it>Schistosoma japonicum</it>
<p>Abstract</p> <p>Background</p> <p>Tetraspanins (TSPs), also known as members of the trans-membrane 4 super-family (TM4SF), comprise an assemblage of surface antigens reported in eukaryotic organisms. In the work presented here, six novel TSP proteins from the human b...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-09-01
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| Series: | Parasites & Vectors |
| Online Access: | http://www.parasitesandvectors.com/content/4/1/190 |
| _version_ | 1819037852897378304 |
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| author | Pan Weiqing Qing Xiaoxing Xu Xindong Jiang Yanyan |
| author_facet | Pan Weiqing Qing Xiaoxing Xu Xindong Jiang Yanyan |
| author_sort | Pan Weiqing |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Tetraspanins (TSPs), also known as members of the trans-membrane 4 super-family (TM4SF), comprise an assemblage of surface antigens reported in eukaryotic organisms. In the work presented here, six novel TSP proteins from the human blood fluke <it>Schistosoma japonicum </it>(<it>S. japonicum</it>) were produced and analyzed through a combination of bioinformatics and experimental approaches.</p> <p>Results</p> <p>Six novel TSP proteins of <it>Schistosoma japonicum </it>(designated as Sj-TSP-#1~6) contained four trans-membrane regions and one large extracellular loop (LEL) with a conserved CCG motif. Size of the proteins varied from 227 to 291 amino acid residues. All the six proteins were produced in <it>E.coli </it>and immune sera to each protein were prepared. Analysis of transcription profiles of the proteins by RT-PCR showed that Sj-TSP-#4 was transcribed only in the egg stage while transcription of the Sj-TSP-#2 was detected in female worms but not in males. The similar results were obtained by Western blot. Immunolocalization of the TSP proteins by immunofluorescence assay showed that the Sj-TSP-#2, Sj-TSP-#5 and Sj-TSP-#6 were located in the tegument of worms.</p> <p>Conclusions</p> <p>This study provided six novel TSP members of <it>S. japonicum </it>including their sequences and recombinant proteins. Availability of the novel proteins and information on their expression profile and location provided a basis for further investigation of the TSP proteins for their biological functions and as vaccine candidates.</p> |
| first_indexed | 2024-12-21T08:28:00Z |
| format | Article |
| id | doaj.art-e561e24aa466413c8249c3dc394e57e1 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-21T08:28:00Z |
| publishDate | 2011-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-e561e24aa466413c8249c3dc394e57e12022-12-21T19:10:17ZengBMCParasites & Vectors1756-33052011-09-014119010.1186/1756-3305-4-190Identification and characterization of six novel tetraspanins from <it>Schistosoma japonicum</it>Pan WeiqingQing XiaoxingXu XindongJiang Yanyan<p>Abstract</p> <p>Background</p> <p>Tetraspanins (TSPs), also known as members of the trans-membrane 4 super-family (TM4SF), comprise an assemblage of surface antigens reported in eukaryotic organisms. In the work presented here, six novel TSP proteins from the human blood fluke <it>Schistosoma japonicum </it>(<it>S. japonicum</it>) were produced and analyzed through a combination of bioinformatics and experimental approaches.</p> <p>Results</p> <p>Six novel TSP proteins of <it>Schistosoma japonicum </it>(designated as Sj-TSP-#1~6) contained four trans-membrane regions and one large extracellular loop (LEL) with a conserved CCG motif. Size of the proteins varied from 227 to 291 amino acid residues. All the six proteins were produced in <it>E.coli </it>and immune sera to each protein were prepared. Analysis of transcription profiles of the proteins by RT-PCR showed that Sj-TSP-#4 was transcribed only in the egg stage while transcription of the Sj-TSP-#2 was detected in female worms but not in males. The similar results were obtained by Western blot. Immunolocalization of the TSP proteins by immunofluorescence assay showed that the Sj-TSP-#2, Sj-TSP-#5 and Sj-TSP-#6 were located in the tegument of worms.</p> <p>Conclusions</p> <p>This study provided six novel TSP members of <it>S. japonicum </it>including their sequences and recombinant proteins. Availability of the novel proteins and information on their expression profile and location provided a basis for further investigation of the TSP proteins for their biological functions and as vaccine candidates.</p>http://www.parasitesandvectors.com/content/4/1/190 |
| spellingShingle | Pan Weiqing Qing Xiaoxing Xu Xindong Jiang Yanyan Identification and characterization of six novel tetraspanins from <it>Schistosoma japonicum</it> Parasites & Vectors |
| title | Identification and characterization of six novel tetraspanins from <it>Schistosoma japonicum</it> |
| title_full | Identification and characterization of six novel tetraspanins from <it>Schistosoma japonicum</it> |
| title_fullStr | Identification and characterization of six novel tetraspanins from <it>Schistosoma japonicum</it> |
| title_full_unstemmed | Identification and characterization of six novel tetraspanins from <it>Schistosoma japonicum</it> |
| title_short | Identification and characterization of six novel tetraspanins from <it>Schistosoma japonicum</it> |
| title_sort | identification and characterization of six novel tetraspanins from it schistosoma japonicum it |
| url | http://www.parasitesandvectors.com/content/4/1/190 |
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