Optimization of Microwave-assisted Extraction of Total Glycosides from Moringa oleifera Seeds and Its Antioxidant Activity
In order to effectively utilize and develop Moringa oleifera seeds, the glycosides in Moringa oleifera seeds were extracted by microwave-assisted method with ethanol as solvent. Based on the single factor experiment, the extraction process was optimized by response surface methodology. The antioxida...
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| Format: | Article |
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The editorial department of Science and Technology of Food Industry
2022-02-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021070078 |
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| author | Xingnan TANG Kaiwen LUAN Rongxiang WANG Shuai REN Wanzhong ZHANG Jianxing ZHU |
| author_facet | Xingnan TANG Kaiwen LUAN Rongxiang WANG Shuai REN Wanzhong ZHANG Jianxing ZHU |
| author_sort | Xingnan TANG |
| collection | DOAJ |
| description | In order to effectively utilize and develop Moringa oleifera seeds, the glycosides in Moringa oleifera seeds were extracted by microwave-assisted method with ethanol as solvent. Based on the single factor experiment, the extraction process was optimized by response surface methodology. The antioxidant activities in vitro of each polar part of the optimized Moringa oleifera seeds extract were determined by salicylic acid method, ABTS method and FRAP method. The results showed that the optimal extraction conditions were as follows: Ethanol volume fraction 81%, solid-liquid ratio 1:30 g/mL, extraction time 20 min, microwave power 600 W. The order of antioxidant activity in vitro was ethyl acetate>petroleum ether > n-butanol > water. The results indicated that the microwave-assisted extraction of glycosides from Moringa oleifera seeds and the optimization of the extraction process of total glycosides from Moringa oleifera seeds by response surface methodology was feasible. In vitro, antioxidant activity tests showed that the polar parts of ethanol extracts of Moringa oleifera seeds had free radical scavenging ability, which laid a foundation for the further development of natural antioxidants. |
| first_indexed | 2024-04-13T09:21:37Z |
| format | Article |
| id | doaj.art-e563af5f58e348c0bb11bdb52dd9ad65 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-13T09:21:37Z |
| publishDate | 2022-02-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| series | Shipin gongye ke-ji |
| spelling | doaj.art-e563af5f58e348c0bb11bdb52dd9ad652022-12-22T02:52:35ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-02-0143424625310.13386/j.issn1002-0306.20210700782021070078-4Optimization of Microwave-assisted Extraction of Total Glycosides from Moringa oleifera Seeds and Its Antioxidant ActivityXingnan TANG0Kaiwen LUAN1Rongxiang WANG2Shuai REN3Wanzhong ZHANG4Jianxing ZHU5College of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology, Shenyang 110142, ChinaCollege of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology, Shenyang 110142, ChinaCollege of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology, Shenyang 110142, ChinaCollege of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology, Shenyang 110142, ChinaCollege of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology, Shenyang 110142, ChinaCollege of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology, Shenyang 110142, ChinaIn order to effectively utilize and develop Moringa oleifera seeds, the glycosides in Moringa oleifera seeds were extracted by microwave-assisted method with ethanol as solvent. Based on the single factor experiment, the extraction process was optimized by response surface methodology. The antioxidant activities in vitro of each polar part of the optimized Moringa oleifera seeds extract were determined by salicylic acid method, ABTS method and FRAP method. The results showed that the optimal extraction conditions were as follows: Ethanol volume fraction 81%, solid-liquid ratio 1:30 g/mL, extraction time 20 min, microwave power 600 W. The order of antioxidant activity in vitro was ethyl acetate>petroleum ether > n-butanol > water. The results indicated that the microwave-assisted extraction of glycosides from Moringa oleifera seeds and the optimization of the extraction process of total glycosides from Moringa oleifera seeds by response surface methodology was feasible. In vitro, antioxidant activity tests showed that the polar parts of ethanol extracts of Moringa oleifera seeds had free radical scavenging ability, which laid a foundation for the further development of natural antioxidants.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021070078moring oleifera seedsresponse surface methodologymicrowave assisted extractiontotal giycosidesantioxidant activity in vitro |
| spellingShingle | Xingnan TANG Kaiwen LUAN Rongxiang WANG Shuai REN Wanzhong ZHANG Jianxing ZHU Optimization of Microwave-assisted Extraction of Total Glycosides from Moringa oleifera Seeds and Its Antioxidant Activity Shipin gongye ke-ji moring oleifera seeds response surface methodology microwave assisted extraction total giycosides antioxidant activity in vitro |
| title | Optimization of Microwave-assisted Extraction of Total Glycosides from Moringa oleifera Seeds and Its Antioxidant Activity |
| title_full | Optimization of Microwave-assisted Extraction of Total Glycosides from Moringa oleifera Seeds and Its Antioxidant Activity |
| title_fullStr | Optimization of Microwave-assisted Extraction of Total Glycosides from Moringa oleifera Seeds and Its Antioxidant Activity |
| title_full_unstemmed | Optimization of Microwave-assisted Extraction of Total Glycosides from Moringa oleifera Seeds and Its Antioxidant Activity |
| title_short | Optimization of Microwave-assisted Extraction of Total Glycosides from Moringa oleifera Seeds and Its Antioxidant Activity |
| title_sort | optimization of microwave assisted extraction of total glycosides from moringa oleifera seeds and its antioxidant activity |
| topic | moring oleifera seeds response surface methodology microwave assisted extraction total giycosides antioxidant activity in vitro |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021070078 |
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