Investigating the Role of Extensin Proteins in Poplar Biomass Recalcitrance
The biological conversion of cellulosic biomass to biofuel is hindered by cell wall recalcitrance, which can limit the ability of cellulases to access and break down cellulose. The purpose of this study was to investigate whether hydroxyproline-rich cell wall proteins (extensins) are present in popl...
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| Format: | Article |
| Language: | English |
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North Carolina State University
2016-04-01
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| Series: | BioResources |
| Subjects: | |
| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_11_2_4727_Fleming_Investigation_Role_Extensin_Proteins |
| _version_ | 1818504879773057024 |
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| author | Margaret Brigham Fleming Stephen R. Decker Patricia A. Bedinger |
| author_facet | Margaret Brigham Fleming Stephen R. Decker Patricia A. Bedinger |
| author_sort | Margaret Brigham Fleming |
| collection | DOAJ |
| description | The biological conversion of cellulosic biomass to biofuel is hindered by cell wall recalcitrance, which can limit the ability of cellulases to access and break down cellulose. The purpose of this study was to investigate whether hydroxyproline-rich cell wall proteins (extensins) are present in poplar stem biomass, and whether these proteins may contribute to recalcitrance. Three classical extensin genes were identified in Populus trichocarpa through bioinformatic analysis of poplar genome sequences, with the following proposed names: PtEXTENSIN1 (Potri.001G019700); PtEXTENSIN2 (Potri.001G020100); PtEXTENSIN3 (Potri.018G050100). Tissue print immunoblots localized the extensin proteins in poplar stems to regions near the vascular cambium. Different thermochemical pretreatments reduced but did not eliminate hydroxyproline (Hyp, a proxy for extensins) from the biomass. Protease treatment of liquid hot water-pretreated poplar biomass reduced Hyp content by a further 16% and increased subsequent glucose yield by 20%. These data suggest that extensins may contribute to recalcitrance in pretreated poplar biomass, and that incorporating protease treatment into pretreatment protocols could result in a small but significant increase in the yield of fermentable glucose. |
| first_indexed | 2024-12-10T21:43:12Z |
| format | Article |
| id | doaj.art-e5646e16c4894351bd042a3789a61b7a |
| institution | Directory Open Access Journal |
| issn | 1930-2126 1930-2126 |
| language | English |
| last_indexed | 2024-12-10T21:43:12Z |
| publishDate | 2016-04-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj.art-e5646e16c4894351bd042a3789a61b7a2022-12-22T01:32:27ZengNorth Carolina State UniversityBioResources1930-21261930-21262016-04-011124727474410.15376/biores.11.2.4727-4744Investigating the Role of Extensin Proteins in Poplar Biomass RecalcitranceMargaret Brigham Fleming0Stephen R. Decker1Patricia A. Bedinger2Colorado State University; United StatesNational Renewable Energy Laboratory; United StatesColorado State University; United StatesThe biological conversion of cellulosic biomass to biofuel is hindered by cell wall recalcitrance, which can limit the ability of cellulases to access and break down cellulose. The purpose of this study was to investigate whether hydroxyproline-rich cell wall proteins (extensins) are present in poplar stem biomass, and whether these proteins may contribute to recalcitrance. Three classical extensin genes were identified in Populus trichocarpa through bioinformatic analysis of poplar genome sequences, with the following proposed names: PtEXTENSIN1 (Potri.001G019700); PtEXTENSIN2 (Potri.001G020100); PtEXTENSIN3 (Potri.018G050100). Tissue print immunoblots localized the extensin proteins in poplar stems to regions near the vascular cambium. Different thermochemical pretreatments reduced but did not eliminate hydroxyproline (Hyp, a proxy for extensins) from the biomass. Protease treatment of liquid hot water-pretreated poplar biomass reduced Hyp content by a further 16% and increased subsequent glucose yield by 20%. These data suggest that extensins may contribute to recalcitrance in pretreated poplar biomass, and that incorporating protease treatment into pretreatment protocols could result in a small but significant increase in the yield of fermentable glucose.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_11_2_4727_Fleming_Investigation_Role_Extensin_ProteinsHydroxyproline-rich glycoproteinCellulosic biofuelBiomass recalcitrancePoplarPretreatmentAcid fungal proteaseExtensin |
| spellingShingle | Margaret Brigham Fleming Stephen R. Decker Patricia A. Bedinger Investigating the Role of Extensin Proteins in Poplar Biomass Recalcitrance BioResources Hydroxyproline-rich glycoprotein Cellulosic biofuel Biomass recalcitrance Poplar Pretreatment Acid fungal protease Extensin |
| title | Investigating the Role of Extensin Proteins in Poplar Biomass Recalcitrance |
| title_full | Investigating the Role of Extensin Proteins in Poplar Biomass Recalcitrance |
| title_fullStr | Investigating the Role of Extensin Proteins in Poplar Biomass Recalcitrance |
| title_full_unstemmed | Investigating the Role of Extensin Proteins in Poplar Biomass Recalcitrance |
| title_short | Investigating the Role of Extensin Proteins in Poplar Biomass Recalcitrance |
| title_sort | investigating the role of extensin proteins in poplar biomass recalcitrance |
| topic | Hydroxyproline-rich glycoprotein Cellulosic biofuel Biomass recalcitrance Poplar Pretreatment Acid fungal protease Extensin |
| url | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_11_2_4727_Fleming_Investigation_Role_Extensin_Proteins |
| work_keys_str_mv | AT margaretbrighamfleming investigatingtheroleofextensinproteinsinpoplarbiomassrecalcitrance AT stephenrdecker investigatingtheroleofextensinproteinsinpoplarbiomassrecalcitrance AT patriciaabedinger investigatingtheroleofextensinproteinsinpoplarbiomassrecalcitrance |