The effects of low-dose 2-hydroxyethyl methacrylate on apoptosis and survival in human dental pulp cells
Background/Purpose: 2-hydroxyethyl methacrylate (HEMA) is one of the most major components in dentin bonding systems. Uncured HEMA is eluted through the dentin and harmful to pulp cells. The study aimed to investigate the death pattern, morphological change and factors of human dental pulp cells (HD...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | Journal of the Formosan Medical Association |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S092966462030601X |
| _version_ | 1818832635371192320 |
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| author | Rung-Shu Chen Ming-Shu Lee Yu-Jen Hu Chia-Yuan Hu Wan-Yu Tseng |
| author_facet | Rung-Shu Chen Ming-Shu Lee Yu-Jen Hu Chia-Yuan Hu Wan-Yu Tseng |
| author_sort | Rung-Shu Chen |
| collection | DOAJ |
| description | Background/Purpose: 2-hydroxyethyl methacrylate (HEMA) is one of the most major components in dentin bonding systems. Uncured HEMA is eluted through the dentin and harmful to pulp cells. The study aimed to investigate the death pattern, morphological change and factors of human dental pulp cells (HDPCs) cultured with low-dose HEMA. Methods: HDPCs were cultured with low-dose concentration of HEMA at 0 mM (control), 0.125 mM, 0.25 mM, 1 mM, 2 mM and 4 mM on Day 3 and 5. The cell morphology was observed with F-actin immunocytochemical staining. The flow cytometry was used to analyze the death pattern. NF-κB and Trx-1 were measured using ELISA kits. Results: The major death pattern was early apoptosis and late apoptosis. The morphological characteristics of apoptosis were observed clearly at 4 mM on Day 3 and Day 5. The phosphorylated NF-κB normalized to total NF-κB protein was significantly higher at 2 mM and 4 mM on Day 5. There was no difference of Trx-1 on Day 3, but significantly higher at 0.25 mM and 1 mM on Day 5. The trend line of phosphorylated NF-κB and Trx-1 showed highly positive correlations with HEMA concentration. Conclusion: The significant cellular morphology characteristics of apoptosis can be observed at higher dose and longer period after exposed to uncured HEMA. The expression of NF-κB was following the ratio of late apoptosis at longer exposure period. Clinically, the remaining dentin thickness should be enough to decrease HEMA concentration and thus to protect pulp cells free from harm. |
| first_indexed | 2024-12-19T02:06:10Z |
| format | Article |
| id | doaj.art-e565d2bd69f34ed09e2fe56d3988877b |
| institution | Directory Open Access Journal |
| issn | 0929-6646 |
| language | English |
| last_indexed | 2024-12-19T02:06:10Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of the Formosan Medical Association |
| spelling | doaj.art-e565d2bd69f34ed09e2fe56d3988877b2022-12-21T20:40:55ZengElsevierJournal of the Formosan Medical Association0929-66462021-06-01120613321339The effects of low-dose 2-hydroxyethyl methacrylate on apoptosis and survival in human dental pulp cellsRung-Shu Chen0Ming-Shu Lee1Yu-Jen Hu2Chia-Yuan Hu3Wan-Yu Tseng4Graduate Institute of Clinical Dentistry, School of Dentistry, College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Dentistry, National Taiwan University Hospital, Taipei, TaiwanGraduate Institute of Clinical Dentistry, School of Dentistry, College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Dentistry, National Taiwan University Hospital, Taipei, TaiwanGraduate Institute of Clinical Dentistry, School of Dentistry, College of Medicine, National Taiwan University, Taipei, Taiwan; Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan; School of Dentistry, College of Medicine, National Taiwan University, Taipei, Taiwan; Corresponding author. School of Dentistry, College of Medicine, National Taiwan University, No.1, Changde Street, Jhongjheng District, Taipei 10048, Taiwan. Fax: +886 2 23831346.Background/Purpose: 2-hydroxyethyl methacrylate (HEMA) is one of the most major components in dentin bonding systems. Uncured HEMA is eluted through the dentin and harmful to pulp cells. The study aimed to investigate the death pattern, morphological change and factors of human dental pulp cells (HDPCs) cultured with low-dose HEMA. Methods: HDPCs were cultured with low-dose concentration of HEMA at 0 mM (control), 0.125 mM, 0.25 mM, 1 mM, 2 mM and 4 mM on Day 3 and 5. The cell morphology was observed with F-actin immunocytochemical staining. The flow cytometry was used to analyze the death pattern. NF-κB and Trx-1 were measured using ELISA kits. Results: The major death pattern was early apoptosis and late apoptosis. The morphological characteristics of apoptosis were observed clearly at 4 mM on Day 3 and Day 5. The phosphorylated NF-κB normalized to total NF-κB protein was significantly higher at 2 mM and 4 mM on Day 5. There was no difference of Trx-1 on Day 3, but significantly higher at 0.25 mM and 1 mM on Day 5. The trend line of phosphorylated NF-κB and Trx-1 showed highly positive correlations with HEMA concentration. Conclusion: The significant cellular morphology characteristics of apoptosis can be observed at higher dose and longer period after exposed to uncured HEMA. The expression of NF-κB was following the ratio of late apoptosis at longer exposure period. Clinically, the remaining dentin thickness should be enough to decrease HEMA concentration and thus to protect pulp cells free from harm.http://www.sciencedirect.com/science/article/pii/S092966462030601XAdhesivesApoptosisDental pulpHydroxyethyl methacrylateNF-kappa B |
| spellingShingle | Rung-Shu Chen Ming-Shu Lee Yu-Jen Hu Chia-Yuan Hu Wan-Yu Tseng The effects of low-dose 2-hydroxyethyl methacrylate on apoptosis and survival in human dental pulp cells Journal of the Formosan Medical Association Adhesives Apoptosis Dental pulp Hydroxyethyl methacrylate NF-kappa B |
| title | The effects of low-dose 2-hydroxyethyl methacrylate on apoptosis and survival in human dental pulp cells |
| title_full | The effects of low-dose 2-hydroxyethyl methacrylate on apoptosis and survival in human dental pulp cells |
| title_fullStr | The effects of low-dose 2-hydroxyethyl methacrylate on apoptosis and survival in human dental pulp cells |
| title_full_unstemmed | The effects of low-dose 2-hydroxyethyl methacrylate on apoptosis and survival in human dental pulp cells |
| title_short | The effects of low-dose 2-hydroxyethyl methacrylate on apoptosis and survival in human dental pulp cells |
| title_sort | effects of low dose 2 hydroxyethyl methacrylate on apoptosis and survival in human dental pulp cells |
| topic | Adhesives Apoptosis Dental pulp Hydroxyethyl methacrylate NF-kappa B |
| url | http://www.sciencedirect.com/science/article/pii/S092966462030601X |
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