Evaluation of the Antioxidant Capacity, Antimicrobial and Antiproliferative Potential of Fir (Abies alba Mill.) Honeydew Honey Collected from Gorski kotar (Croatia)
The paper examines the antiproliferative, antimicrobial and antioxidative effects of fir (Abies alba Mill.) honeydew honey from mountain region of Croatia (Gorski kotar) as a potential replacement for standard antibiotics and chemotherapeutic agents. Cell viability, annexin V assay and flow cytometr...
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| Format: | Article |
| Language: | English |
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University of Zagreb Faculty of Food Technology and Biotechnology
2018-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | https://hrcak.srce.hr/file/314244 |
| _version_ | 1827282791214088192 |
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| author | Dalibor Broznić Ivana Ratkaj Mladenka Malenica Staver Sandra Kraljević Pavelić Paula Žurga Dragan Bubalo Ivana Gobin |
| author_facet | Dalibor Broznić Ivana Ratkaj Mladenka Malenica Staver Sandra Kraljević Pavelić Paula Žurga Dragan Bubalo Ivana Gobin |
| author_sort | Dalibor Broznić |
| collection | DOAJ |
| description | The paper examines the antiproliferative, antimicrobial and antioxidative effects of fir (Abies alba Mill.) honeydew honey from mountain region of Croatia (Gorski kotar) as a potential replacement for standard antibiotics and chemotherapeutic agents. Cell viability, annexin V assay and flow cytometry analysis served to analyse the antiproliferative effect on, apoptosis induction in and cell death of cancer cell lines: HeLa, MCF-7, SW620, CFPAC-1, MIA PaCa-2 and normal diploid human fibroblasts (BJ). Antimicrobial activity was tested against Staphylococcus and Acinetobacter strains by agar well diffusion and microdilution assays. The DPPH˙ assay determined the radical scavenging activity, while mathematical models helped to evaluate the kinetic data of DPPH˙ inhibition. Antiproliferative effect on all tested cell lines and the prominent effect on normal diploid human fibroblasts (BJ), colorectal adenocarcinoma (SW620, metastatic) and breast epithelial adenocarcinoma (MCF-7, metastatic) was observable. The mechanisms of antiproliferative effect included accumulation of cells in the sub-G1 phase in all tested cells and induction of apoptosis in SW620 and MCF-7 cells predominantly. The antibacterial assays showed that antibiotic resistant strains of both bacteria, including multi-resistant strain A. baumannii ATCC® BAA-1605™, were sensitive to all tested honey samples. Radical scavenging assay suggests that antioxidants present in the honey possess different radical suppressing abilities and that they react at different rates with radicals, thereby causing two steps of reaction. The results of the study indicate that Croatian fir honeydew honey has a therapeutic potential due to the strong biological activity and can serve to protect human health. |
| first_indexed | 2024-04-24T09:25:10Z |
| format | Article |
| id | doaj.art-e566246b3a5341ae9d1d0b863c0d5c1f |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-04-24T09:25:10Z |
| publishDate | 2018-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-e566246b3a5341ae9d1d0b863c0d5c1f2024-04-15T15:15:11ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062018-01-0156453354510.17113/ftb.56.04.18.5666Evaluation of the Antioxidant Capacity, Antimicrobial and Antiproliferative Potential of Fir (Abies alba Mill.) Honeydew Honey Collected from Gorski kotar (Croatia)Dalibor Broznić0Ivana Ratkaj1Mladenka Malenica Staver2Sandra Kraljević Pavelić3Paula Žurga4Dragan Bubalo5Ivana Gobin6Department of Chemistry and Biochemistry, Faculty of Medicine, University of Rijeka, Braće Branchetta 20, HR-51000 Rijeka, CroatiaDepartment of Biotechnology, University of Rijeka, Radmile Matejčić 2, HR-51000 Rijeka, CroatiaDepartment of Biotechnology, University of Rijeka, Radmile Matejčić 2, HR-51000 Rijeka, CroatiaDepartment of Biotechnology, Centre for High Throughput Technologies, University of Rijeka, Radmile Matejčić 2, HR-51000 Rijeka, CroatiaTeaching Institute of Public Health of Primorsko-goranska County, Krešimirova 52a, HR-51000 Rijeka, CroatiaFaculty of Agriculture, University of Zagreb, Svetošimunska 25, HR-10000 Zagreb, CroatiaDepartment of Microbiology, Faculty of Medicine, University of Rijeka, Braće Branchetta 20, HR-51000 Rijeka, CroatiaThe paper examines the antiproliferative, antimicrobial and antioxidative effects of fir (Abies alba Mill.) honeydew honey from mountain region of Croatia (Gorski kotar) as a potential replacement for standard antibiotics and chemotherapeutic agents. Cell viability, annexin V assay and flow cytometry analysis served to analyse the antiproliferative effect on, apoptosis induction in and cell death of cancer cell lines: HeLa, MCF-7, SW620, CFPAC-1, MIA PaCa-2 and normal diploid human fibroblasts (BJ). Antimicrobial activity was tested against Staphylococcus and Acinetobacter strains by agar well diffusion and microdilution assays. The DPPH˙ assay determined the radical scavenging activity, while mathematical models helped to evaluate the kinetic data of DPPH˙ inhibition. Antiproliferative effect on all tested cell lines and the prominent effect on normal diploid human fibroblasts (BJ), colorectal adenocarcinoma (SW620, metastatic) and breast epithelial adenocarcinoma (MCF-7, metastatic) was observable. The mechanisms of antiproliferative effect included accumulation of cells in the sub-G1 phase in all tested cells and induction of apoptosis in SW620 and MCF-7 cells predominantly. The antibacterial assays showed that antibiotic resistant strains of both bacteria, including multi-resistant strain A. baumannii ATCC® BAA-1605™, were sensitive to all tested honey samples. Radical scavenging assay suggests that antioxidants present in the honey possess different radical suppressing abilities and that they react at different rates with radicals, thereby causing two steps of reaction. The results of the study indicate that Croatian fir honeydew honey has a therapeutic potential due to the strong biological activity and can serve to protect human health.https://hrcak.srce.hr/file/314244fir (Abies alba Mill.) honeydew honeycell cycleapoptosisM antimicrobial activityantioxidant capacitykinetic analysis |
| spellingShingle | Dalibor Broznić Ivana Ratkaj Mladenka Malenica Staver Sandra Kraljević Pavelić Paula Žurga Dragan Bubalo Ivana Gobin Evaluation of the Antioxidant Capacity, Antimicrobial and Antiproliferative Potential of Fir (Abies alba Mill.) Honeydew Honey Collected from Gorski kotar (Croatia) Food Technology and Biotechnology fir (Abies alba Mill.) honeydew honey cell cycle apoptosisM antimicrobial activity antioxidant capacity kinetic analysis |
| title | Evaluation of the Antioxidant Capacity, Antimicrobial and Antiproliferative Potential of Fir (Abies alba Mill.) Honeydew Honey Collected from Gorski kotar (Croatia) |
| title_full | Evaluation of the Antioxidant Capacity, Antimicrobial and Antiproliferative Potential of Fir (Abies alba Mill.) Honeydew Honey Collected from Gorski kotar (Croatia) |
| title_fullStr | Evaluation of the Antioxidant Capacity, Antimicrobial and Antiproliferative Potential of Fir (Abies alba Mill.) Honeydew Honey Collected from Gorski kotar (Croatia) |
| title_full_unstemmed | Evaluation of the Antioxidant Capacity, Antimicrobial and Antiproliferative Potential of Fir (Abies alba Mill.) Honeydew Honey Collected from Gorski kotar (Croatia) |
| title_short | Evaluation of the Antioxidant Capacity, Antimicrobial and Antiproliferative Potential of Fir (Abies alba Mill.) Honeydew Honey Collected from Gorski kotar (Croatia) |
| title_sort | evaluation of the antioxidant capacity antimicrobial and antiproliferative potential of fir abies alba mill honeydew honey collected from gorski kotar croatia |
| topic | fir (Abies alba Mill.) honeydew honey cell cycle apoptosisM antimicrobial activity antioxidant capacity kinetic analysis |
| url | https://hrcak.srce.hr/file/314244 |
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