Active Microbiota of <i>Penaeus stylirostris</i> Larvae: Partially Shaped via Vertical and Horizontal Transmissions and Larval Ontogeny
During their entire lifecycle, mariculture animals are farmed in water that contains various microorganisms with which they are in close associations. Microbial exchanges between the animals and their surrounding water can occur. However, little is known about the interactions between shrimp larvae...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/12/3/608 |
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| author | Nolwenn Callac Carolane Giraud Dominique Pham Dominique Ansquer Nelly Wabete Viviane Boulo |
| author_facet | Nolwenn Callac Carolane Giraud Dominique Pham Dominique Ansquer Nelly Wabete Viviane Boulo |
| author_sort | Nolwenn Callac |
| collection | DOAJ |
| description | During their entire lifecycle, mariculture animals are farmed in water that contains various microorganisms with which they are in close associations. Microbial exchanges between the animals and their surrounding water can occur. However, little is known about the interactions between shrimp larvae and water, and more especially, about larval bacterial selection and microbiota modulation across ontogeny. To address this gap, using HiSeq sequencing targeting the V4 region of the 16S rRNA molecule, we investigated the active prokaryotic diversity and structure of healthy <i>Penaeus stylirostris</i> larvae and seawater. Comparisons between different larval stages revealed evidence of stage-specific microbiotas and biomarkers, a core microbiota common to all stages, and shared taxa between successive stages, suggesting vertical transmission of bacterial taxa. Comparisons between stage-specific microbiotas and core microbiotas with water storages highlighted that many taxa associated with the larvae were originally present in the natural seawater, underlining horizontal transmission of bacteria from water to larvae. As some of these lineages became active at specific larval stages, we suggest that larvae were able to modulate their microbiota. This study provides insight into larvae-microbiota interactions at the larval stage scale. |
| first_indexed | 2024-04-24T17:59:54Z |
| format | Article |
| id | doaj.art-e5684ce574864648b4cd121eb7d42ba7 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-04-24T17:59:54Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-e5684ce574864648b4cd121eb7d42ba72024-03-27T13:55:59ZengMDPI AGMicroorganisms2076-26072024-03-0112360810.3390/microorganisms12030608Active Microbiota of <i>Penaeus stylirostris</i> Larvae: Partially Shaped via Vertical and Horizontal Transmissions and Larval OntogenyNolwenn Callac0Carolane Giraud1Dominique Pham2Dominique Ansquer3Nelly Wabete4Viviane Boulo5Ifremer, IRD, Université de la Nouvelle-Calédonie, Université de La Réunion, CNRS, UMR 9220 ENTROPIE, 101 Promenade Roger Laroque, 98897 Nouméa, New CaledoniaIfremer, IRD, Université de la Nouvelle-Calédonie, Université de La Réunion, CNRS, UMR 9220 ENTROPIE, 101 Promenade Roger Laroque, 98897 Nouméa, New CaledoniaIfremer, IRD, Université de la Nouvelle-Calédonie, Université de La Réunion, CNRS, UMR 9220 ENTROPIE, 101 Promenade Roger Laroque, 98897 Nouméa, New CaledoniaIfremer, IRD, Université de la Nouvelle-Calédonie, Université de La Réunion, CNRS, UMR 9220 ENTROPIE, 101 Promenade Roger Laroque, 98897 Nouméa, New CaledoniaIfremer, IRD, Université de la Nouvelle-Calédonie, Université de La Réunion, CNRS, UMR 9220 ENTROPIE, 101 Promenade Roger Laroque, 98897 Nouméa, New CaledoniaIfremer, IRD, Université de la Nouvelle-Calédonie, Université de La Réunion, CNRS, UMR 9220 ENTROPIE, 101 Promenade Roger Laroque, 98897 Nouméa, New CaledoniaDuring their entire lifecycle, mariculture animals are farmed in water that contains various microorganisms with which they are in close associations. Microbial exchanges between the animals and their surrounding water can occur. However, little is known about the interactions between shrimp larvae and water, and more especially, about larval bacterial selection and microbiota modulation across ontogeny. To address this gap, using HiSeq sequencing targeting the V4 region of the 16S rRNA molecule, we investigated the active prokaryotic diversity and structure of healthy <i>Penaeus stylirostris</i> larvae and seawater. Comparisons between different larval stages revealed evidence of stage-specific microbiotas and biomarkers, a core microbiota common to all stages, and shared taxa between successive stages, suggesting vertical transmission of bacterial taxa. Comparisons between stage-specific microbiotas and core microbiotas with water storages highlighted that many taxa associated with the larvae were originally present in the natural seawater, underlining horizontal transmission of bacteria from water to larvae. As some of these lineages became active at specific larval stages, we suggest that larvae were able to modulate their microbiota. This study provides insight into larvae-microbiota interactions at the larval stage scale.https://www.mdpi.com/2076-2607/12/3/608active microbiotashrimplarvaetaxa transmissionspecific microbiotabiomarkers |
| spellingShingle | Nolwenn Callac Carolane Giraud Dominique Pham Dominique Ansquer Nelly Wabete Viviane Boulo Active Microbiota of <i>Penaeus stylirostris</i> Larvae: Partially Shaped via Vertical and Horizontal Transmissions and Larval Ontogeny Microorganisms active microbiota shrimp larvae taxa transmission specific microbiota biomarkers |
| title | Active Microbiota of <i>Penaeus stylirostris</i> Larvae: Partially Shaped via Vertical and Horizontal Transmissions and Larval Ontogeny |
| title_full | Active Microbiota of <i>Penaeus stylirostris</i> Larvae: Partially Shaped via Vertical and Horizontal Transmissions and Larval Ontogeny |
| title_fullStr | Active Microbiota of <i>Penaeus stylirostris</i> Larvae: Partially Shaped via Vertical and Horizontal Transmissions and Larval Ontogeny |
| title_full_unstemmed | Active Microbiota of <i>Penaeus stylirostris</i> Larvae: Partially Shaped via Vertical and Horizontal Transmissions and Larval Ontogeny |
| title_short | Active Microbiota of <i>Penaeus stylirostris</i> Larvae: Partially Shaped via Vertical and Horizontal Transmissions and Larval Ontogeny |
| title_sort | active microbiota of i penaeus stylirostris i larvae partially shaped via vertical and horizontal transmissions and larval ontogeny |
| topic | active microbiota shrimp larvae taxa transmission specific microbiota biomarkers |
| url | https://www.mdpi.com/2076-2607/12/3/608 |
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