Subcellular Localization of Fad1p in <i>Saccharomyces cerevisiae</i>: A Choice at Post-Transcriptional Level?
FAD synthase is the last enzyme in the pathway that converts riboflavin into FAD. In <i>Saccharomyces cerevisiae</i>, the gene encoding for FAD synthase is <i>FAD1</i>, from which a sole protein product (Fad1p) is expected to be generated. In this work, we showed that a natur...
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2021-09-01
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| author | Francesco Bruni Teresa Anna Giancaspero Mislav Oreb Maria Tolomeo Piero Leone Eckhard Boles Marina Roberti Michele Caselle Maria Barile |
| author_facet | Francesco Bruni Teresa Anna Giancaspero Mislav Oreb Maria Tolomeo Piero Leone Eckhard Boles Marina Roberti Michele Caselle Maria Barile |
| author_sort | Francesco Bruni |
| collection | DOAJ |
| description | FAD synthase is the last enzyme in the pathway that converts riboflavin into FAD. In <i>Saccharomyces cerevisiae</i>, the gene encoding for FAD synthase is <i>FAD1</i>, from which a sole protein product (Fad1p) is expected to be generated. In this work, we showed that a natural Fad1p exists in yeast mitochondria and that, in its recombinant form, the protein is able, per se, to both enter mitochondria and to be destined to cytosol. Thus, we propose that <i>FAD1</i> generates two echoforms—that is, two identical proteins addressed to different subcellular compartments. To shed light on the mechanism underlying the subcellular destination of Fad1p, the 3′ region of <i>FAD1</i> mRNA was analyzed by 3′RACE experiments, which revealed the existence of (at least) two <i>FAD1</i> transcripts with different 3′UTRs, the short one being 128 bp and the long one being 759 bp. Bioinformatic analysis on these 3′UTRs allowed us to predict the existence of a <i>cis</i>-acting mitochondrial localization motif, present in both the transcripts and, presumably, involved in protein targeting based on the 3′UTR context. Here, we propose that the long <i>FAD1</i> transcript might be responsible for the generation of mitochondrial Fad1p echoform. |
| first_indexed | 2024-03-10T07:30:26Z |
| format | Article |
| id | doaj.art-e5697caf7b5544678c19ce5c772586fb |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T07:30:26Z |
| publishDate | 2021-09-01 |
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| series | Life |
| spelling | doaj.art-e5697caf7b5544678c19ce5c772586fb2023-11-22T13:56:37ZengMDPI AGLife2075-17292021-09-0111996710.3390/life11090967Subcellular Localization of Fad1p in <i>Saccharomyces cerevisiae</i>: A Choice at Post-Transcriptional Level?Francesco Bruni0Teresa Anna Giancaspero1Mislav Oreb2Maria Tolomeo3Piero Leone4Eckhard Boles5Marina Roberti6Michele Caselle7Maria Barile8Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari Aldo Moro, Via Orabona 4, 70125 Bari, ItalyDepartment of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari Aldo Moro, Via Orabona 4, 70125 Bari, ItalyInstitute of Molecular Biosciences, Goethe-University Frankfurt, Max-von-Laue-Str. 9, 60438 Frankfurt am Main, GermanyDepartment of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari Aldo Moro, Via Orabona 4, 70125 Bari, ItalyDepartment of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari Aldo Moro, Via Orabona 4, 70125 Bari, ItalyInstitute of Molecular Biosciences, Goethe-University Frankfurt, Max-von-Laue-Str. 9, 60438 Frankfurt am Main, GermanyDepartment of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari Aldo Moro, Via Orabona 4, 70125 Bari, ItalyPhysics Department, University of Turin and INFN, Via P. Giuria 1, 10125 Turin, ItalyDepartment of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari Aldo Moro, Via Orabona 4, 70125 Bari, ItalyFAD synthase is the last enzyme in the pathway that converts riboflavin into FAD. In <i>Saccharomyces cerevisiae</i>, the gene encoding for FAD synthase is <i>FAD1</i>, from which a sole protein product (Fad1p) is expected to be generated. In this work, we showed that a natural Fad1p exists in yeast mitochondria and that, in its recombinant form, the protein is able, per se, to both enter mitochondria and to be destined to cytosol. Thus, we propose that <i>FAD1</i> generates two echoforms—that is, two identical proteins addressed to different subcellular compartments. To shed light on the mechanism underlying the subcellular destination of Fad1p, the 3′ region of <i>FAD1</i> mRNA was analyzed by 3′RACE experiments, which revealed the existence of (at least) two <i>FAD1</i> transcripts with different 3′UTRs, the short one being 128 bp and the long one being 759 bp. Bioinformatic analysis on these 3′UTRs allowed us to predict the existence of a <i>cis</i>-acting mitochondrial localization motif, present in both the transcripts and, presumably, involved in protein targeting based on the 3′UTR context. Here, we propose that the long <i>FAD1</i> transcript might be responsible for the generation of mitochondrial Fad1p echoform.https://www.mdpi.com/2075-1729/11/9/967FAD synthase<i>FAD1</i>mitochondria localization<i>Saccharomyces cerevisiae</i>mRNAmitochondrial localization motif |
| spellingShingle | Francesco Bruni Teresa Anna Giancaspero Mislav Oreb Maria Tolomeo Piero Leone Eckhard Boles Marina Roberti Michele Caselle Maria Barile Subcellular Localization of Fad1p in <i>Saccharomyces cerevisiae</i>: A Choice at Post-Transcriptional Level? Life FAD synthase <i>FAD1</i> mitochondria localization <i>Saccharomyces cerevisiae</i> mRNA mitochondrial localization motif |
| title | Subcellular Localization of Fad1p in <i>Saccharomyces cerevisiae</i>: A Choice at Post-Transcriptional Level? |
| title_full | Subcellular Localization of Fad1p in <i>Saccharomyces cerevisiae</i>: A Choice at Post-Transcriptional Level? |
| title_fullStr | Subcellular Localization of Fad1p in <i>Saccharomyces cerevisiae</i>: A Choice at Post-Transcriptional Level? |
| title_full_unstemmed | Subcellular Localization of Fad1p in <i>Saccharomyces cerevisiae</i>: A Choice at Post-Transcriptional Level? |
| title_short | Subcellular Localization of Fad1p in <i>Saccharomyces cerevisiae</i>: A Choice at Post-Transcriptional Level? |
| title_sort | subcellular localization of fad1p in i saccharomyces cerevisiae i a choice at post transcriptional level |
| topic | FAD synthase <i>FAD1</i> mitochondria localization <i>Saccharomyces cerevisiae</i> mRNA mitochondrial localization motif |
| url | https://www.mdpi.com/2075-1729/11/9/967 |
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