Long Non-coding RNAs Contribute to the Inhibition of Proliferation and EMT by Pterostilbene in Human Breast Cancer
Background: There is increasing evidence that long non-coding RNAs (lncRNAs) are involved in the process of carcinogenesis and treatment using chemotherapy. Pterostilbene, a phytochemical agent with natural antioxidant and anti-inflammatory properties, has been shown to modulate oncogenic processes...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-12-01
|
| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fonc.2018.00629/full |
| _version_ | 1811204697616285696 |
|---|---|
| author | Yongye Huang Juan Du Yan Mi Tianye Li Ying Gong Hongsheng Ouyang Yue Hou |
| author_facet | Yongye Huang Juan Du Yan Mi Tianye Li Ying Gong Hongsheng Ouyang Yue Hou |
| author_sort | Yongye Huang |
| collection | DOAJ |
| description | Background: There is increasing evidence that long non-coding RNAs (lncRNAs) are involved in the process of carcinogenesis and treatment using chemotherapy. Pterostilbene, a phytochemical agent with natural antioxidant and anti-inflammatory properties, has been shown to modulate oncogenic processes in many cancers. However, there has been limited research on the association between pterostilbene and the expression of lncRNAs.Methods: MCF7 breast cancer cells were treated with various concentrations of pterostilbene and their gene expression profile was analyzed by quantitative real-time PCR, Western blotting and immunofluorescence.Results: Treatment with pterostilbene inhibited cell proliferation and epithelial-to-mesenchymal transition (EMT), and increased cell apoptosis, autophagy and ER stress. The Akt/mTOR pathway was downregulated, but p38 MAPK/Erk signaling was activated in cells following treatment with pterostilbene. Pterostilbene increased the expression of the lncRNAs MEG3, TUG1, H19, and DICER1-AS1 whereas the expression of LINC01121, PTTG3P, and HOTAIR declined. Knockdown of lncRNA H19 resulted in a reduction of the cell invasion, with the cells becoming more sensitive to pterostilbene therapy.Conclusions: These results suggest that efficient optimum disruption of lncRNA expression might possibly improve the anti-tumor effects of phytochemical agents, thus serving as a potential therapy for breast cancer. |
| first_indexed | 2024-04-12T03:16:55Z |
| format | Article |
| id | doaj.art-e56f5cac52b24f65bb2447f2e83c15a1 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-12T03:16:55Z |
| publishDate | 2018-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-e56f5cac52b24f65bb2447f2e83c15a12022-12-22T03:50:04ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2018-12-01810.3389/fonc.2018.00629426626Long Non-coding RNAs Contribute to the Inhibition of Proliferation and EMT by Pterostilbene in Human Breast CancerYongye Huang0Juan Du1Yan Mi2Tianye Li3Ying Gong4Hongsheng Ouyang5Yue Hou6College of Life and Health Sciences, Northeastern University, Shenyang, ChinaCollege of Life and Health Sciences, Northeastern University, Shenyang, ChinaCollege of Life and Health Sciences, Northeastern University, Shenyang, ChinaCollege of Life and Health Sciences, Northeastern University, Shenyang, ChinaCollege of Life and Health Sciences, Northeastern University, Shenyang, ChinaJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, ChinaCollege of Life and Health Sciences, Northeastern University, Shenyang, ChinaBackground: There is increasing evidence that long non-coding RNAs (lncRNAs) are involved in the process of carcinogenesis and treatment using chemotherapy. Pterostilbene, a phytochemical agent with natural antioxidant and anti-inflammatory properties, has been shown to modulate oncogenic processes in many cancers. However, there has been limited research on the association between pterostilbene and the expression of lncRNAs.Methods: MCF7 breast cancer cells were treated with various concentrations of pterostilbene and their gene expression profile was analyzed by quantitative real-time PCR, Western blotting and immunofluorescence.Results: Treatment with pterostilbene inhibited cell proliferation and epithelial-to-mesenchymal transition (EMT), and increased cell apoptosis, autophagy and ER stress. The Akt/mTOR pathway was downregulated, but p38 MAPK/Erk signaling was activated in cells following treatment with pterostilbene. Pterostilbene increased the expression of the lncRNAs MEG3, TUG1, H19, and DICER1-AS1 whereas the expression of LINC01121, PTTG3P, and HOTAIR declined. Knockdown of lncRNA H19 resulted in a reduction of the cell invasion, with the cells becoming more sensitive to pterostilbene therapy.Conclusions: These results suggest that efficient optimum disruption of lncRNA expression might possibly improve the anti-tumor effects of phytochemical agents, thus serving as a potential therapy for breast cancer.https://www.frontiersin.org/article/10.3389/fonc.2018.00629/fullpterostilbenelncRNAscancerautophagyepithelial-to-mesenchymal transition |
| spellingShingle | Yongye Huang Juan Du Yan Mi Tianye Li Ying Gong Hongsheng Ouyang Yue Hou Long Non-coding RNAs Contribute to the Inhibition of Proliferation and EMT by Pterostilbene in Human Breast Cancer Frontiers in Oncology pterostilbene lncRNAs cancer autophagy epithelial-to-mesenchymal transition |
| title | Long Non-coding RNAs Contribute to the Inhibition of Proliferation and EMT by Pterostilbene in Human Breast Cancer |
| title_full | Long Non-coding RNAs Contribute to the Inhibition of Proliferation and EMT by Pterostilbene in Human Breast Cancer |
| title_fullStr | Long Non-coding RNAs Contribute to the Inhibition of Proliferation and EMT by Pterostilbene in Human Breast Cancer |
| title_full_unstemmed | Long Non-coding RNAs Contribute to the Inhibition of Proliferation and EMT by Pterostilbene in Human Breast Cancer |
| title_short | Long Non-coding RNAs Contribute to the Inhibition of Proliferation and EMT by Pterostilbene in Human Breast Cancer |
| title_sort | long non coding rnas contribute to the inhibition of proliferation and emt by pterostilbene in human breast cancer |
| topic | pterostilbene lncRNAs cancer autophagy epithelial-to-mesenchymal transition |
| url | https://www.frontiersin.org/article/10.3389/fonc.2018.00629/full |
| work_keys_str_mv | AT yongyehuang longnoncodingrnascontributetotheinhibitionofproliferationandemtbypterostilbeneinhumanbreastcancer AT juandu longnoncodingrnascontributetotheinhibitionofproliferationandemtbypterostilbeneinhumanbreastcancer AT yanmi longnoncodingrnascontributetotheinhibitionofproliferationandemtbypterostilbeneinhumanbreastcancer AT tianyeli longnoncodingrnascontributetotheinhibitionofproliferationandemtbypterostilbeneinhumanbreastcancer AT yinggong longnoncodingrnascontributetotheinhibitionofproliferationandemtbypterostilbeneinhumanbreastcancer AT hongshengouyang longnoncodingrnascontributetotheinhibitionofproliferationandemtbypterostilbeneinhumanbreastcancer AT yuehou longnoncodingrnascontributetotheinhibitionofproliferationandemtbypterostilbeneinhumanbreastcancer |