AuNPs Enhanced Microwave Coupled Lipase Synthesis of Starch Oleate Ester
The nano-gold immobilized lipase (CAL@AuNPs) was prepared to synthesize starch oleate ester efficiently by using pre-treated corn starch and oleic acid as raw materials. Optimisation of the preparation conditions of CAL@AuNPs was carried out using oleic acid conversion rate as an indicator. The subs...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
The editorial department of Science and Technology of Food Industry
2022-12-01
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| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022020183 |
| _version_ | 1811317068015861760 |
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| author | Yan WANG Hongjia LI Meijia CHENG Jinhui XIE Tianjiao LIU Jiaying XIN Na ZHANG |
| author_facet | Yan WANG Hongjia LI Meijia CHENG Jinhui XIE Tianjiao LIU Jiaying XIN Na ZHANG |
| author_sort | Yan WANG |
| collection | DOAJ |
| description | The nano-gold immobilized lipase (CAL@AuNPs) was prepared to synthesize starch oleate ester efficiently by using pre-treated corn starch and oleic acid as raw materials. Optimisation of the preparation conditions of CAL@AuNPs was carried out using oleic acid conversion rate as an indicator. The substitution degree was used as an indicator to investigate the catalytic efficiency of free enzyme, commercial immobilised enzyme and nanogold immobilised lipase under microwave-assisted conditions. The experimental results showed that the immobilized lipase with the strongest catalytic activity could be obtained by using AuNPs of 14 nm as carrier at 40 ℃ for 6 h. The starch oleate ester with the highest substitution degree of 0.0259 could be prepared by using CAL@AuNPs as catalyst under the condition of 400 W, 35 ℃ for 40 min. Compared with free enzyme, and commercial immobilized enzyme , the CAL@AuNPs could shorten reaction time and improve the degree of substitution. |
| first_indexed | 2024-04-13T12:01:02Z |
| format | Article |
| id | doaj.art-e56ff61111bf4bb78ad5237422a1fb3b |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-13T12:01:02Z |
| publishDate | 2022-12-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-e56ff61111bf4bb78ad5237422a1fb3b2022-12-22T02:47:47ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-12-01432320020910.13386/j.issn1002-0306.20220201832022020183-23AuNPs Enhanced Microwave Coupled Lipase Synthesis of Starch Oleate EsterYan WANG0Hongjia LI1Meijia CHENG2Jinhui XIE3Tianjiao LIU4Jiaying XIN5Na ZHANG6Key Laboratory of Food Science and Engineering, Harbin University of Commerce, Harbin 150028, ChinaKey Laboratory of Food Science and Engineering, Harbin University of Commerce, Harbin 150028, ChinaKey Laboratory of Food Science and Engineering, Harbin University of Commerce, Harbin 150028, ChinaKey Laboratory of Food Science and Engineering, Harbin University of Commerce, Harbin 150028, ChinaKey Laboratory of Food Science and Engineering, Harbin University of Commerce, Harbin 150028, ChinaKey Laboratory of Food Science and Engineering, Harbin University of Commerce, Harbin 150028, ChinaKey Laboratory of Food Science and Engineering, Harbin University of Commerce, Harbin 150028, ChinaThe nano-gold immobilized lipase (CAL@AuNPs) was prepared to synthesize starch oleate ester efficiently by using pre-treated corn starch and oleic acid as raw materials. Optimisation of the preparation conditions of CAL@AuNPs was carried out using oleic acid conversion rate as an indicator. The substitution degree was used as an indicator to investigate the catalytic efficiency of free enzyme, commercial immobilised enzyme and nanogold immobilised lipase under microwave-assisted conditions. The experimental results showed that the immobilized lipase with the strongest catalytic activity could be obtained by using AuNPs of 14 nm as carrier at 40 ℃ for 6 h. The starch oleate ester with the highest substitution degree of 0.0259 could be prepared by using CAL@AuNPs as catalyst under the condition of 400 W, 35 ℃ for 40 min. Compared with free enzyme, and commercial immobilized enzyme , the CAL@AuNPs could shorten reaction time and improve the degree of substitution.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022020183lipase immobilized by aunpsmicrowave assisted lipase catalysis technologystarch oleate esteroleic acid conversion ratedegree of substitution |
| spellingShingle | Yan WANG Hongjia LI Meijia CHENG Jinhui XIE Tianjiao LIU Jiaying XIN Na ZHANG AuNPs Enhanced Microwave Coupled Lipase Synthesis of Starch Oleate Ester Shipin gongye ke-ji lipase immobilized by aunps microwave assisted lipase catalysis technology starch oleate ester oleic acid conversion rate degree of substitution |
| title | AuNPs Enhanced Microwave Coupled Lipase Synthesis of Starch Oleate Ester |
| title_full | AuNPs Enhanced Microwave Coupled Lipase Synthesis of Starch Oleate Ester |
| title_fullStr | AuNPs Enhanced Microwave Coupled Lipase Synthesis of Starch Oleate Ester |
| title_full_unstemmed | AuNPs Enhanced Microwave Coupled Lipase Synthesis of Starch Oleate Ester |
| title_short | AuNPs Enhanced Microwave Coupled Lipase Synthesis of Starch Oleate Ester |
| title_sort | aunps enhanced microwave coupled lipase synthesis of starch oleate ester |
| topic | lipase immobilized by aunps microwave assisted lipase catalysis technology starch oleate ester oleic acid conversion rate degree of substitution |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022020183 |
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