Altered Thermal Behavior of Blood Plasma Proteome Related to Inflammatory Cytokines in Early Pregnancy Loss
Early pregnancy loss (EPL) is a relatively common pathology of which almost 50% of cases remain idiopathic. In the search for novel biomarkers, differential scanning calorimetry (DSC) is intensively used to characterize the thermodynamic behavior of blood plasma/serum proteome in health and disease....
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/15/8764 |
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| author | Regina Komsa-Penkova Avgustina Danailova Sashka Krumova Galya Georgieva Ina Giosheva Lidia Gartcheva Ivan Iliev Emil Gartchev Kameliya Kercheva Alexey Savov Svetla Todinova |
| author_facet | Regina Komsa-Penkova Avgustina Danailova Sashka Krumova Galya Georgieva Ina Giosheva Lidia Gartcheva Ivan Iliev Emil Gartchev Kameliya Kercheva Alexey Savov Svetla Todinova |
| author_sort | Regina Komsa-Penkova |
| collection | DOAJ |
| description | Early pregnancy loss (EPL) is a relatively common pathology of which almost 50% of cases remain idiopathic. In the search for novel biomarkers, differential scanning calorimetry (DSC) is intensively used to characterize the thermodynamic behavior of blood plasma/serum proteome in health and disease. Herein, for the first time, we investigate the DSC denaturation profiles of blood plasma derived from patients suffering EPL compared to healthy pregnant and non-pregnant women. Data analysis reveals that 58% of the EPL thermograms differ significantly from those of healthy pregnant women. Thermal stabilization of a fraction of albumin-assigned transition with concomitant suppression of the major and enhancement of the globulin-assigned transition are characteristic features of EPL calorimetric profiles that could be used as a new indicator of a risk pregnancy. The presented results suggest an altered composition or intermolecular interactions of the plasma proteome of women with EPL. In addition, the alterations of the EPL thermograms correlate with the increased blood levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) and a higher prevalence of the polymorphism in the plasminogen activator inhibitor type-1 (<i>PAI-1)</i> gene, suggesting an expression of an overall enhanced immune response. The concomitant changes in plasma thermograms confirm the potential of the DSC approach for distinguishing changes in the pathological state of the blood plasma proteome. |
| first_indexed | 2024-03-09T10:07:56Z |
| format | Article |
| id | doaj.art-e570314ace4a4a07b0be75de780a6a68 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T10:07:56Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e570314ace4a4a07b0be75de780a6a682023-12-01T22:58:45ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012315876410.3390/ijms23158764Altered Thermal Behavior of Blood Plasma Proteome Related to Inflammatory Cytokines in Early Pregnancy LossRegina Komsa-Penkova0Avgustina Danailova1Sashka Krumova2Galya Georgieva3Ina Giosheva4Lidia Gartcheva5Ivan Iliev6Emil Gartchev7Kameliya Kercheva8Alexey Savov9Svetla Todinova10Department of Biochemistry, Medical University—Pleven, Sv. Kliment Ohridski Str. 1, 5800 Pleven, BulgariaInstitute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, “Acad. G. Bontchev” Str. 21, 1113 Sofia, BulgariaInstitute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, “Acad. G. Bontchev” Str. 21, 1113 Sofia, BulgariaDepartment of Biochemistry, Medical University—Pleven, Sv. Kliment Ohridski Str. 1, 5800 Pleven, BulgariaInstitute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, “Acad. G. Bontchev” Str. 21, 1113 Sofia, BulgariaNational Specialized Hospital for Active Treating of Haematological Diseases, 1756 Sofia, BulgariaInstitute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, “Acad. G. Bontchev” Str. 21, 1113 Sofia, BulgariaUniversity Hospital of Obstetrics and Gynecology “Maichin Dom”, Medical University Sofia, Zdrave Str. 2, 1431 Sofia, BulgariaUniversity Hospital of Obstetrics and Gynecology “Maichin Dom”, Medical University Sofia, Zdrave Str. 2, 1431 Sofia, BulgariaUniversity Hospital of Obstetrics and Gynecology “Maichin Dom”, Medical University Sofia, Zdrave Str. 2, 1431 Sofia, BulgariaInstitute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, “Acad. G. Bontchev” Str. 21, 1113 Sofia, BulgariaEarly pregnancy loss (EPL) is a relatively common pathology of which almost 50% of cases remain idiopathic. In the search for novel biomarkers, differential scanning calorimetry (DSC) is intensively used to characterize the thermodynamic behavior of blood plasma/serum proteome in health and disease. Herein, for the first time, we investigate the DSC denaturation profiles of blood plasma derived from patients suffering EPL compared to healthy pregnant and non-pregnant women. Data analysis reveals that 58% of the EPL thermograms differ significantly from those of healthy pregnant women. Thermal stabilization of a fraction of albumin-assigned transition with concomitant suppression of the major and enhancement of the globulin-assigned transition are characteristic features of EPL calorimetric profiles that could be used as a new indicator of a risk pregnancy. The presented results suggest an altered composition or intermolecular interactions of the plasma proteome of women with EPL. In addition, the alterations of the EPL thermograms correlate with the increased blood levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) and a higher prevalence of the polymorphism in the plasminogen activator inhibitor type-1 (<i>PAI-1)</i> gene, suggesting an expression of an overall enhanced immune response. The concomitant changes in plasma thermograms confirm the potential of the DSC approach for distinguishing changes in the pathological state of the blood plasma proteome.https://www.mdpi.com/1422-0067/23/15/8764blood plasma proteomeearly pregnancy lossdifferential scanning calorimetry4G/5G polymorphism in <i>PAI-1</i> genetumor necrosis factor αinterleukin-6 |
| spellingShingle | Regina Komsa-Penkova Avgustina Danailova Sashka Krumova Galya Georgieva Ina Giosheva Lidia Gartcheva Ivan Iliev Emil Gartchev Kameliya Kercheva Alexey Savov Svetla Todinova Altered Thermal Behavior of Blood Plasma Proteome Related to Inflammatory Cytokines in Early Pregnancy Loss International Journal of Molecular Sciences blood plasma proteome early pregnancy loss differential scanning calorimetry 4G/5G polymorphism in <i>PAI-1</i> gene tumor necrosis factor α interleukin-6 |
| title | Altered Thermal Behavior of Blood Plasma Proteome Related to Inflammatory Cytokines in Early Pregnancy Loss |
| title_full | Altered Thermal Behavior of Blood Plasma Proteome Related to Inflammatory Cytokines in Early Pregnancy Loss |
| title_fullStr | Altered Thermal Behavior of Blood Plasma Proteome Related to Inflammatory Cytokines in Early Pregnancy Loss |
| title_full_unstemmed | Altered Thermal Behavior of Blood Plasma Proteome Related to Inflammatory Cytokines in Early Pregnancy Loss |
| title_short | Altered Thermal Behavior of Blood Plasma Proteome Related to Inflammatory Cytokines in Early Pregnancy Loss |
| title_sort | altered thermal behavior of blood plasma proteome related to inflammatory cytokines in early pregnancy loss |
| topic | blood plasma proteome early pregnancy loss differential scanning calorimetry 4G/5G polymorphism in <i>PAI-1</i> gene tumor necrosis factor α interleukin-6 |
| url | https://www.mdpi.com/1422-0067/23/15/8764 |
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