Early Identification and Antimicrobial Susceptibility Testing of Short versus Standard Incubated Blood Cultures from a Tertiary Care Centre in Southern India
Introduction: Blood cultures play an important role in the early diagnosis of sepsis and its management. Early detection of pathogens in Blood Stream Infections (BSI) and their Antimicrobial Susceptibility Testing (AST) pattern, plays a vital role in the diagnosis of sepsis and is important for...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2022-02-01
|
| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/16004/52679_CE(Ra1)_F(SS)_PF1(AG_SS)_PFA(AG_KM)_PN(KM).pdf |
| Summary: | Introduction: Blood cultures play an important role in the early
diagnosis of sepsis and its management. Early detection of
pathogens in Blood Stream Infections (BSI) and their Antimicrobial
Susceptibility Testing (AST) pattern, plays a vital role in the
diagnosis of sepsis and is important for guidance of appropriate
therapy.
Aim: To evaluate the accuracy of shortly incubated blood cultures
in comparison with standard method for an early Identification
(ID) and AST.
Materials and Methods: This was a prospective observational
study undertaken from July 2015 to June 2016 at Nizam’s
Institute of Medical Sciences, Hyderabad, Telangana, India. The
blood cultures were loaded in the BacT/Alert system. A total of
92 patients with two sets of blood cultures that flagged positive
within 24 hours of collection were included in the study. Grams
stain and subcultures of the broths were done. The culture plates
were examined after four hours and then at hourly intervals for
the presence of growth. Once the growth was sufficient it was
processed immediately for ID and AST by Vitek 2C. Incubation
of the plates was continued for the rest of the 24 hours at 37oC
and was processed again. The mean time for detection were
compared between short and standard cultures.
Results: Gram negative pathogens were the predominant organisms
isolated in 82/92 (89.1%) followed by gram positive in 10/92 (10.9%).
The short and standard cultures had comparable results with respect
to ID of the isolates. But, the AST results were comparable only in
88/92 (95.6%) patients. Of the remaining four patients, the AST
showed Very Major Error (VME) in 3 (3.3%) patients and Major Error
(ME) in 1 (1.08%) patient.
Conclusion: Short incubation of cultures enabled earliest
ID and AST of the isolates from blood cultures than standard
incubation. |
|---|---|
| ISSN: | 2249-782X 0973-709X |