Early Identification and Antimicrobial Susceptibility Testing of Short versus Standard Incubated Blood Cultures from a Tertiary Care Centre in Southern India
Introduction: Blood cultures play an important role in the early diagnosis of sepsis and its management. Early detection of pathogens in Blood Stream Infections (BSI) and their Antimicrobial Susceptibility Testing (AST) pattern, plays a vital role in the diagnosis of sepsis and is important for...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2022-02-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/16004/52679_CE(Ra1)_F(SS)_PF1(AG_SS)_PFA(AG_KM)_PN(KM).pdf |
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| author | Kanne Padmaja Sukanya Sudhaharan Lakshmi Vemu Vijay Dharma Teja |
| author_facet | Kanne Padmaja Sukanya Sudhaharan Lakshmi Vemu Vijay Dharma Teja |
| author_sort | Kanne Padmaja |
| collection | DOAJ |
| description | Introduction: Blood cultures play an important role in the early
diagnosis of sepsis and its management. Early detection of
pathogens in Blood Stream Infections (BSI) and their Antimicrobial
Susceptibility Testing (AST) pattern, plays a vital role in the
diagnosis of sepsis and is important for guidance of appropriate
therapy.
Aim: To evaluate the accuracy of shortly incubated blood cultures
in comparison with standard method for an early Identification
(ID) and AST.
Materials and Methods: This was a prospective observational
study undertaken from July 2015 to June 2016 at Nizam’s
Institute of Medical Sciences, Hyderabad, Telangana, India. The
blood cultures were loaded in the BacT/Alert system. A total of
92 patients with two sets of blood cultures that flagged positive
within 24 hours of collection were included in the study. Grams
stain and subcultures of the broths were done. The culture plates
were examined after four hours and then at hourly intervals for
the presence of growth. Once the growth was sufficient it was
processed immediately for ID and AST by Vitek 2C. Incubation
of the plates was continued for the rest of the 24 hours at 37oC
and was processed again. The mean time for detection were
compared between short and standard cultures.
Results: Gram negative pathogens were the predominant organisms
isolated in 82/92 (89.1%) followed by gram positive in 10/92 (10.9%).
The short and standard cultures had comparable results with respect
to ID of the isolates. But, the AST results were comparable only in
88/92 (95.6%) patients. Of the remaining four patients, the AST
showed Very Major Error (VME) in 3 (3.3%) patients and Major Error
(ME) in 1 (1.08%) patient.
Conclusion: Short incubation of cultures enabled earliest
ID and AST of the isolates from blood cultures than standard
incubation. |
| first_indexed | 2024-12-21T04:37:38Z |
| format | Article |
| id | doaj.art-e573b17b6e674471b9520cecb59c851e |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-21T04:37:38Z |
| publishDate | 2022-02-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-e573b17b6e674471b9520cecb59c851e2022-12-21T19:15:49ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2022-02-01162DC20DC2410.7860/JCDR/2022/52679.16004Early Identification and Antimicrobial Susceptibility Testing of Short versus Standard Incubated Blood Cultures from a Tertiary Care Centre in Southern IndiaKanne Padmaja0Sukanya Sudhaharan1Lakshmi Vemu2Vijay Dharma Teja3Associate Professor, Department of Microbiology, Nizam’s Institute of Medical Sciences, Hyderabad, Telangana, India.Associate Professor, Department of Microbiology, Nizam’s Institute of Medical Sciences, Hyderabad, Telangana, India.Professor, Department of Microbiology, Nizam’s Institute of Medical Sciences, Hyderabad, Telangana, India.Professor and Head, Department of Microbiology, Nizam’s Institute of Medical Sciences, Hyderabad, Telangana, India.Introduction: Blood cultures play an important role in the early diagnosis of sepsis and its management. Early detection of pathogens in Blood Stream Infections (BSI) and their Antimicrobial Susceptibility Testing (AST) pattern, plays a vital role in the diagnosis of sepsis and is important for guidance of appropriate therapy. Aim: To evaluate the accuracy of shortly incubated blood cultures in comparison with standard method for an early Identification (ID) and AST. Materials and Methods: This was a prospective observational study undertaken from July 2015 to June 2016 at Nizam’s Institute of Medical Sciences, Hyderabad, Telangana, India. The blood cultures were loaded in the BacT/Alert system. A total of 92 patients with two sets of blood cultures that flagged positive within 24 hours of collection were included in the study. Grams stain and subcultures of the broths were done. The culture plates were examined after four hours and then at hourly intervals for the presence of growth. Once the growth was sufficient it was processed immediately for ID and AST by Vitek 2C. Incubation of the plates was continued for the rest of the 24 hours at 37oC and was processed again. The mean time for detection were compared between short and standard cultures. Results: Gram negative pathogens were the predominant organisms isolated in 82/92 (89.1%) followed by gram positive in 10/92 (10.9%). The short and standard cultures had comparable results with respect to ID of the isolates. But, the AST results were comparable only in 88/92 (95.6%) patients. Of the remaining four patients, the AST showed Very Major Error (VME) in 3 (3.3%) patients and Major Error (ME) in 1 (1.08%) patient. Conclusion: Short incubation of cultures enabled earliest ID and AST of the isolates from blood cultures than standard incubation.https://jcdr.net/articles/PDF/16004/52679_CE(Ra1)_F(SS)_PF1(AG_SS)_PFA(AG_KM)_PN(KM).pdfblood stream infectionsconcordancevery major error |
| spellingShingle | Kanne Padmaja Sukanya Sudhaharan Lakshmi Vemu Vijay Dharma Teja Early Identification and Antimicrobial Susceptibility Testing of Short versus Standard Incubated Blood Cultures from a Tertiary Care Centre in Southern India Journal of Clinical and Diagnostic Research blood stream infections concordance very major error |
| title | Early Identification and Antimicrobial Susceptibility Testing of Short versus Standard Incubated Blood Cultures from a Tertiary Care Centre in Southern India |
| title_full | Early Identification and Antimicrobial Susceptibility Testing of Short versus Standard Incubated Blood Cultures from a Tertiary Care Centre in Southern India |
| title_fullStr | Early Identification and Antimicrobial Susceptibility Testing of Short versus Standard Incubated Blood Cultures from a Tertiary Care Centre in Southern India |
| title_full_unstemmed | Early Identification and Antimicrobial Susceptibility Testing of Short versus Standard Incubated Blood Cultures from a Tertiary Care Centre in Southern India |
| title_short | Early Identification and Antimicrobial Susceptibility Testing of Short versus Standard Incubated Blood Cultures from a Tertiary Care Centre in Southern India |
| title_sort | early identification and antimicrobial susceptibility testing of short versus standard incubated blood cultures from a tertiary care centre in southern india |
| topic | blood stream infections concordance very major error |
| url | https://jcdr.net/articles/PDF/16004/52679_CE(Ra1)_F(SS)_PF1(AG_SS)_PFA(AG_KM)_PN(KM).pdf |
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