Thermoactinomyces sacchari competent strain: Isolation from compost, selection and characterization for biotechnological use
Composting is an uncontrolled process of converting organic waste into a beneficial soil conditioner for agriculture. During this process, a diverse community of micro-organisms, including thermophilic bacteria and actinomycetes, plays an essential role in organic matter decomposition. These micro-o...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Scientific African |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468227624000668 |
| _version_ | 1797277320888713216 |
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| author | Beroigui Oumaima Chadli Nour-Eddine Raoui S. Mohammed Abdelkrim Ouammou Chedadi Oussama Errachidi Faouzi |
| author_facet | Beroigui Oumaima Chadli Nour-Eddine Raoui S. Mohammed Abdelkrim Ouammou Chedadi Oussama Errachidi Faouzi |
| author_sort | Beroigui Oumaima |
| collection | DOAJ |
| description | Composting is an uncontrolled process of converting organic waste into a beneficial soil conditioner for agriculture. During this process, a diverse community of micro-organisms, including thermophilic bacteria and actinomycetes, plays an essential role in organic matter decomposition. These micro-organisms are capable of thriving at high temperatures, and have therefore received increasing attention due to their potential industrial applications such as biotechnology and bioremediation.This study aims to isolate competent thermophilic actinomycetes to control and optimize composting process. Samples were taken during the three phases of composting process characterized by temperature fluctuations. Classical identification, morphological and biochemical, of thermophilic actinomycetes strains was carried out to distinguish strains with interesting metabolic power. Selection pressure exerted by different temperatures (50 °C, 55 °C and 60 °C) on six competents strains led to the selection of a strain best suited to extreme composting conditions. Its taxonomic position was verified using molecular tools.This study, developed within a biological engineering framework, highlights the role of using the composting process in a controlled bioreactor, which can standardize physico-chemical and nutritional conditions to ensure composting reproducibility. Thermophilic strains, including Thermoactinomycetes Sacchari, form a consortium well adapted to the conditions imposed by the bioreactor.Thus, this study highlights the role that certain strains can play in the success of the composting process, particularly those that are still present in the active composting population.The isolation of Thermoactinomycetes sacchari strain provides some clues to the phenomenon of controlled composting boosted by a competent strain under industrial conditions. Thanks to its resistance to high osmotic pressures (20–60 g/L), a wide range of hydronium potentials (pH 5–10) and high temperatures, Thermoactinomycetes sacchari strain is a candidate for producing enzymes of biotechnological interest that can be exploited under the most rigorous industrial conditions. This strain could be the subject of research and development on the molecules produced by this microorganism, which has a wide range of biological activities. |
| first_indexed | 2024-03-07T15:46:58Z |
| format | Article |
| id | doaj.art-e575db26a1ea40afa78d7617f1985b1d |
| institution | Directory Open Access Journal |
| issn | 2468-2276 |
| language | English |
| last_indexed | 2024-03-07T15:46:58Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Scientific African |
| spelling | doaj.art-e575db26a1ea40afa78d7617f1985b1d2024-03-05T04:30:43ZengElsevierScientific African2468-22762024-03-0123e02121Thermoactinomyces sacchari competent strain: Isolation from compost, selection and characterization for biotechnological useBeroigui Oumaima0Chadli Nour-Eddine1Raoui S. Mohammed2Abdelkrim Ouammou3Chedadi Oussama4Errachidi Faouzi5Departement of Biology, Functional Ecology and Environmental Engineering Laboratory, Faculty of Science and Technology, University Sidi Mohammed Ben Abdellah, Fez 30000, Morocco; Corresponding author.Department of Biology, Immunology and biodiversity Laboratory, Ain Chock Faculty of Sciences, University Hassan II, Casablanca 20100, MoroccoHigher Institute of Nursing Professions and Health Techniques of Meknes, MoroccoLIMOME Laboratory, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Fez, MoroccoLIMOME Laboratory, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Fez, MoroccoDepartement of Biology, Functional Ecology and Environmental Engineering Laboratory, Faculty of Science and Technology, University Sidi Mohammed Ben Abdellah, Fez 30000, MoroccoComposting is an uncontrolled process of converting organic waste into a beneficial soil conditioner for agriculture. During this process, a diverse community of micro-organisms, including thermophilic bacteria and actinomycetes, plays an essential role in organic matter decomposition. These micro-organisms are capable of thriving at high temperatures, and have therefore received increasing attention due to their potential industrial applications such as biotechnology and bioremediation.This study aims to isolate competent thermophilic actinomycetes to control and optimize composting process. Samples were taken during the three phases of composting process characterized by temperature fluctuations. Classical identification, morphological and biochemical, of thermophilic actinomycetes strains was carried out to distinguish strains with interesting metabolic power. Selection pressure exerted by different temperatures (50 °C, 55 °C and 60 °C) on six competents strains led to the selection of a strain best suited to extreme composting conditions. Its taxonomic position was verified using molecular tools.This study, developed within a biological engineering framework, highlights the role of using the composting process in a controlled bioreactor, which can standardize physico-chemical and nutritional conditions to ensure composting reproducibility. Thermophilic strains, including Thermoactinomycetes Sacchari, form a consortium well adapted to the conditions imposed by the bioreactor.Thus, this study highlights the role that certain strains can play in the success of the composting process, particularly those that are still present in the active composting population.The isolation of Thermoactinomycetes sacchari strain provides some clues to the phenomenon of controlled composting boosted by a competent strain under industrial conditions. Thanks to its resistance to high osmotic pressures (20–60 g/L), a wide range of hydronium potentials (pH 5–10) and high temperatures, Thermoactinomycetes sacchari strain is a candidate for producing enzymes of biotechnological interest that can be exploited under the most rigorous industrial conditions. This strain could be the subject of research and development on the molecules produced by this microorganism, which has a wide range of biological activities.http://www.sciencedirect.com/science/article/pii/S2468227624000668Thermophilic actinomycetesBioreactorComposting processDegrading enzymes and molecular identification |
| spellingShingle | Beroigui Oumaima Chadli Nour-Eddine Raoui S. Mohammed Abdelkrim Ouammou Chedadi Oussama Errachidi Faouzi Thermoactinomyces sacchari competent strain: Isolation from compost, selection and characterization for biotechnological use Scientific African Thermophilic actinomycetes Bioreactor Composting process Degrading enzymes and molecular identification |
| title | Thermoactinomyces sacchari competent strain: Isolation from compost, selection and characterization for biotechnological use |
| title_full | Thermoactinomyces sacchari competent strain: Isolation from compost, selection and characterization for biotechnological use |
| title_fullStr | Thermoactinomyces sacchari competent strain: Isolation from compost, selection and characterization for biotechnological use |
| title_full_unstemmed | Thermoactinomyces sacchari competent strain: Isolation from compost, selection and characterization for biotechnological use |
| title_short | Thermoactinomyces sacchari competent strain: Isolation from compost, selection and characterization for biotechnological use |
| title_sort | thermoactinomyces sacchari competent strain isolation from compost selection and characterization for biotechnological use |
| topic | Thermophilic actinomycetes Bioreactor Composting process Degrading enzymes and molecular identification |
| url | http://www.sciencedirect.com/science/article/pii/S2468227624000668 |
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