Host-dependence of in vitro reassortment dynamics among the Sathuperi and Shamonda Simbuviruses
ABSTRACTOrthobunyaviruses are arboviruses (Arthropod Borne Virus) and possess multipartite genomes made up of three negative RNAs corresponding to the small (S), medium (M) and large (L) segments. Reassortment and recombination are evolutionary driving forces of such segmented viruses and lead to th...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Emerging Microbes and Infections |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2019.1586410 |
| _version_ | 1797385801948987392 |
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| author | Damien Coupeau Calixte Bayrou Pierre Baillieux Axel Marichal Anne-Cécile Lenaerts Céline Caty Laetitia Wiggers Nathalie Kirschvink Daniel Desmecht Benoît Muylkens |
| author_facet | Damien Coupeau Calixte Bayrou Pierre Baillieux Axel Marichal Anne-Cécile Lenaerts Céline Caty Laetitia Wiggers Nathalie Kirschvink Daniel Desmecht Benoît Muylkens |
| author_sort | Damien Coupeau |
| collection | DOAJ |
| description | ABSTRACTOrthobunyaviruses are arboviruses (Arthropod Borne Virus) and possess multipartite genomes made up of three negative RNAs corresponding to the small (S), medium (M) and large (L) segments. Reassortment and recombination are evolutionary driving forces of such segmented viruses and lead to the emergence of new strains and species. Retrospective studies based on phylogenetical analysis are able to evaluate these mechanisms at the end of the selection process but fail to address the dynamics of emergence. This issue was addressed using two Orthobunyaviruses infecting ruminants and belonging to the Simbu serogroup: the Sathuperi virus (SATV) and the Shamonda virus (SHAV). Both viruses were associated with abortion, stillbirth and congenital malformations occurring after transplacental transmission and were suspected to spread together in different ruminant and insect populations. This study showed that different viruses related to SHAV and SATV are spreading simultaneously in ruminants and equids of the Sub-Saharan region. Their reassortment and recombination potential was evaluated in mammalian and in insect contexts. A method was set up to determine the genomic background of any clonal progeny viruses isolated after in vitro coinfections assays. All the reassortment combinations were generated in both contexts while no recombinant virus was isolated. Progeny virus populations revealed a high level of reassortment in mammalian cells and a much lower level in insect cells. In vitro selection pressure that mimicked the host switching (insect-mammal) revealed that the best adapted reassortant virus was connected with an advantageous replicative fitness and with the presence of a specific segment. |
| first_indexed | 2024-03-08T21:59:36Z |
| format | Article |
| id | doaj.art-e5771d252c494351bdc3994053bacd9b |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-03-08T21:59:36Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-e5771d252c494351bdc3994053bacd9b2023-12-19T16:09:57ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512019-01-018138139510.1080/22221751.2019.1586410Host-dependence of in vitro reassortment dynamics among the Sathuperi and Shamonda SimbuvirusesDamien Coupeau0Calixte Bayrou1Pierre Baillieux2Axel Marichal3Anne-Cécile Lenaerts4Céline Caty5Laetitia Wiggers6Nathalie Kirschvink7Daniel Desmecht8Benoît Muylkens9Veterinary Department, Faculty of Sciences, Namur Research Institute for Life Sciences (NARILIS), University of Namur (UNamur) Namur, BelgiumDepartment of Morphology and Pathology, FARAH Research Center, Faculty of Veterinary Medicine, University of Liège Liège, BelgiumVeterinary Department, Faculty of Sciences, Namur Research Institute for Life Sciences (NARILIS), University of Namur (UNamur) Namur, BelgiumVeterinary Department, Faculty of Sciences, Namur Research Institute for Life Sciences (NARILIS), University of Namur (UNamur) Namur, BelgiumVeterinary Department, Faculty of Sciences, Namur Research Institute for Life Sciences (NARILIS), University of Namur (UNamur) Namur, BelgiumVeterinary Department, Faculty of Sciences, Namur Research Institute for Life Sciences (NARILIS), University of Namur (UNamur) Namur, BelgiumVeterinary Department, Faculty of Sciences, Namur Research Institute for Life Sciences (NARILIS), University of Namur (UNamur) Namur, BelgiumVeterinary Department, Faculty of Sciences, Namur Research Institute for Life Sciences (NARILIS), University of Namur (UNamur) Namur, BelgiumDepartment of Morphology and Pathology, FARAH Research Center, Faculty of Veterinary Medicine, University of Liège Liège, BelgiumVeterinary Department, Faculty of Sciences, Namur Research Institute for Life Sciences (NARILIS), University of Namur (UNamur) Namur, BelgiumABSTRACTOrthobunyaviruses are arboviruses (Arthropod Borne Virus) and possess multipartite genomes made up of three negative RNAs corresponding to the small (S), medium (M) and large (L) segments. Reassortment and recombination are evolutionary driving forces of such segmented viruses and lead to the emergence of new strains and species. Retrospective studies based on phylogenetical analysis are able to evaluate these mechanisms at the end of the selection process but fail to address the dynamics of emergence. This issue was addressed using two Orthobunyaviruses infecting ruminants and belonging to the Simbu serogroup: the Sathuperi virus (SATV) and the Shamonda virus (SHAV). Both viruses were associated with abortion, stillbirth and congenital malformations occurring after transplacental transmission and were suspected to spread together in different ruminant and insect populations. This study showed that different viruses related to SHAV and SATV are spreading simultaneously in ruminants and equids of the Sub-Saharan region. Their reassortment and recombination potential was evaluated in mammalian and in insect contexts. A method was set up to determine the genomic background of any clonal progeny viruses isolated after in vitro coinfections assays. All the reassortment combinations were generated in both contexts while no recombinant virus was isolated. Progeny virus populations revealed a high level of reassortment in mammalian cells and a much lower level in insect cells. In vitro selection pressure that mimicked the host switching (insect-mammal) revealed that the best adapted reassortant virus was connected with an advantageous replicative fitness and with the presence of a specific segment.https://www.tandfonline.com/doi/10.1080/22221751.2019.1586410Orthobunyavirusreassortmentrecombinationviral selectionarbovirus |
| spellingShingle | Damien Coupeau Calixte Bayrou Pierre Baillieux Axel Marichal Anne-Cécile Lenaerts Céline Caty Laetitia Wiggers Nathalie Kirschvink Daniel Desmecht Benoît Muylkens Host-dependence of in vitro reassortment dynamics among the Sathuperi and Shamonda Simbuviruses Emerging Microbes and Infections Orthobunyavirus reassortment recombination viral selection arbovirus |
| title | Host-dependence of in vitro reassortment dynamics among the Sathuperi and Shamonda Simbuviruses |
| title_full | Host-dependence of in vitro reassortment dynamics among the Sathuperi and Shamonda Simbuviruses |
| title_fullStr | Host-dependence of in vitro reassortment dynamics among the Sathuperi and Shamonda Simbuviruses |
| title_full_unstemmed | Host-dependence of in vitro reassortment dynamics among the Sathuperi and Shamonda Simbuviruses |
| title_short | Host-dependence of in vitro reassortment dynamics among the Sathuperi and Shamonda Simbuviruses |
| title_sort | host dependence of in vitro reassortment dynamics among the sathuperi and shamonda simbuviruses |
| topic | Orthobunyavirus reassortment recombination viral selection arbovirus |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2019.1586410 |
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