Molecular characterization and antimicrobial resistance of faecal and urinary Escherichia coli isolated from dogs and humans in Italy
During this study, 109 faecal Escherichia coli samples isolated from 61 dogs and 48 humans were characterised according to phylogenetic group, extraintestinal virulence factors and antibiotic resistance. The isolates from dogs were predominantly distributed within phylogroup B1 (36%), while the majo...
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| Format: | Article |
| Language: | English |
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Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2014-03-01
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| Series: | Veterinaria Italiana |
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| Online Access: | http://www.izs.it/vet_italiana/2014/50_1/VetIt_1304_09.pdf |
| _version_ | 1828745029879857152 |
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| author | Clara Tramuta Patrizia Robino Daniele Nucera Sara Salvarani Giuliana Banche Aurelio Malabaila Patrizia Nebbia |
| author_facet | Clara Tramuta Patrizia Robino Daniele Nucera Sara Salvarani Giuliana Banche Aurelio Malabaila Patrizia Nebbia |
| author_sort | Clara Tramuta |
| collection | DOAJ |
| description | During this study, 109 faecal Escherichia coli samples isolated from 61 dogs and 48 humans were characterised according to phylogenetic group, extraintestinal virulence factors and antibiotic resistance. The isolates from dogs were predominantly distributed within phylogroup B1 (36%), while the majority of human strains belonged to phylogroup B2 (54%). The prevalence of cnf1, hlyA, papC and sfa virulence genes was significantly associated with the group B2. Canine isolates showed multidrug resistance (MDR) more frequently than human strains. Since group B2 contains most of the strains that cause extraintestinal infections, all 46 B2 faecal strains were confronted against an addition population of 57 urinary E. coli strains belonging to the same phylogroup. The comparison shows that there was no significant difference in the occurrence of virulence factors or in the distribution of antibiotic resistance between faecal and urinary E. coli isolates fromd dogs. At the same time, a highly significant association was detected between multiple resistence and the source of the strains and between MDR and E. coli isolated from urine in human. This study highlighted similar features of E. coli isolated across sources and hosts. The data suggest a high prevalence of antibiotic resistance in faecal strains, which may represent a serious health risk since these strains can function as a reservoir for uropathogenic E. coli. |
| first_indexed | 2024-04-14T03:54:54Z |
| format | Article |
| id | doaj.art-e57c6bad28ae42b89127bdc02ef5a42f |
| institution | Directory Open Access Journal |
| issn | 0505-401X 1828-1427 |
| language | English |
| last_indexed | 2024-04-14T03:54:54Z |
| publishDate | 2014-03-01 |
| publisher | Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale |
| record_format | Article |
| series | Veterinaria Italiana |
| spelling | doaj.art-e57c6bad28ae42b89127bdc02ef5a42f2022-12-22T02:13:51ZengIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. CaporaleVeterinaria Italiana0505-401X1828-14272014-03-01501233010.12834/VetIt.1304.09Molecular characterization and antimicrobial resistance of faecal and urinary Escherichia coli isolated from dogs and humans in ItalyClara Tramuta0Patrizia Robino1Daniele Nucera2Sara Salvarani3Giuliana Banche4Aurelio Malabaila5Patrizia Nebbia6Department of Veterinary Sciences, University of Turin, Via Leonardo da Vinci 44, 10095 Grugliasco, Torino, ItalyDepartment of Veterinary Sciences, University of Turin, Via Leonardo da Vinci 44, 10095 Grugliasco, Torino, ItalyDepartment of Agricultural, Forestry and Food Sciences, University of Turin, Via Leonardo da Vinci 44, 10095 Grugliasco, Torino, ItalyDepartment of Veterinary Sciences, University of Turin, Via Leonardo da Vinci 44, 10095 Grugliasco, Torino, ItalyDepartment of Public Health and Microbiology, University of Turin, Via Santena 9, 10126 Torino, ItalyAnalysis Laboratory and Microbiology, Infermi Hospital, Via Caraccio 5, 13900 Biella, ItalyDepartment of Veterinary Sciences, University of Turin, Via Leonardo da Vinci 44, 10095 Grugliasco, Torino, Italy. Tel.: +39 011 6709188, e-mail: patrizia.nebbia@unito.itDuring this study, 109 faecal Escherichia coli samples isolated from 61 dogs and 48 humans were characterised according to phylogenetic group, extraintestinal virulence factors and antibiotic resistance. The isolates from dogs were predominantly distributed within phylogroup B1 (36%), while the majority of human strains belonged to phylogroup B2 (54%). The prevalence of cnf1, hlyA, papC and sfa virulence genes was significantly associated with the group B2. Canine isolates showed multidrug resistance (MDR) more frequently than human strains. Since group B2 contains most of the strains that cause extraintestinal infections, all 46 B2 faecal strains were confronted against an addition population of 57 urinary E. coli strains belonging to the same phylogroup. The comparison shows that there was no significant difference in the occurrence of virulence factors or in the distribution of antibiotic resistance between faecal and urinary E. coli isolates fromd dogs. At the same time, a highly significant association was detected between multiple resistence and the source of the strains and between MDR and E. coli isolated from urine in human. This study highlighted similar features of E. coli isolated across sources and hosts. The data suggest a high prevalence of antibiotic resistance in faecal strains, which may represent a serious health risk since these strains can function as a reservoir for uropathogenic E. coli.http://www.izs.it/vet_italiana/2014/50_1/VetIt_1304_09.pdfAntibiotic resistanceDogEscherichia coliHumanPhylogenetic groupVirulence factors |
| spellingShingle | Clara Tramuta Patrizia Robino Daniele Nucera Sara Salvarani Giuliana Banche Aurelio Malabaila Patrizia Nebbia Molecular characterization and antimicrobial resistance of faecal and urinary Escherichia coli isolated from dogs and humans in Italy Veterinaria Italiana Antibiotic resistance Dog Escherichia coli Human Phylogenetic group Virulence factors |
| title | Molecular characterization and antimicrobial resistance of faecal and urinary Escherichia coli isolated from dogs and humans in Italy |
| title_full | Molecular characterization and antimicrobial resistance of faecal and urinary Escherichia coli isolated from dogs and humans in Italy |
| title_fullStr | Molecular characterization and antimicrobial resistance of faecal and urinary Escherichia coli isolated from dogs and humans in Italy |
| title_full_unstemmed | Molecular characterization and antimicrobial resistance of faecal and urinary Escherichia coli isolated from dogs and humans in Italy |
| title_short | Molecular characterization and antimicrobial resistance of faecal and urinary Escherichia coli isolated from dogs and humans in Italy |
| title_sort | molecular characterization and antimicrobial resistance of faecal and urinary escherichia coli isolated from dogs and humans in italy |
| topic | Antibiotic resistance Dog Escherichia coli Human Phylogenetic group Virulence factors |
| url | http://www.izs.it/vet_italiana/2014/50_1/VetIt_1304_09.pdf |
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