Quantitative Detection of the Influenza a Virus by an EGOFET-Based Portable Device
Elaboration of biosensors on the base of organic transistors with embedded biomolecules which can operate in an aqueous environment is of paramount importance. Electrolyte-gated organic field-effect transistors demonstrate high sensitivity in detection of various analytes. In this paper, we demonstr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/11/8/464 |
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| author | Elena Y. Poimanova Elena G. Zavyalova Elena A. Kretova Anton A. Abramov Askold A. Trul Oleg V. Borshchev Anna K. Keshek Sergey A. Ponomarenko Elena V. Agina |
| author_facet | Elena Y. Poimanova Elena G. Zavyalova Elena A. Kretova Anton A. Abramov Askold A. Trul Oleg V. Borshchev Anna K. Keshek Sergey A. Ponomarenko Elena V. Agina |
| author_sort | Elena Y. Poimanova |
| collection | DOAJ |
| description | Elaboration of biosensors on the base of organic transistors with embedded biomolecules which can operate in an aqueous environment is of paramount importance. Electrolyte-gated organic field-effect transistors demonstrate high sensitivity in detection of various analytes. In this paper, we demonstrated the possibility of quantitative fast specific determination of virus particles by an aptasensor based on EGOFET. The sensitivity and selectivity of the devices were examined with the influenza A virus as well as with control bioliquids like influenza B, Newcastle disease viruses or allantoic fluid with different dilutions. The influence of the semiconducting layer thickness on EGOFETs sensory properties is discussed. The fabrication of a multi-flow cell that simultaneously registers the responses from several devices on the same substrate and the creation of a multi-sensor flow device are reported. The responses of the elaborated bioelectronic platform to the influenza A virus obtained with application of the portable multi-flow mode are well correlated with the responses obtained in the laboratory stationary mode. |
| first_indexed | 2024-03-11T00:02:51Z |
| format | Article |
| id | doaj.art-e5823cc8df474cbbab9351b8fd1aad59 |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-11T00:02:51Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-e5823cc8df474cbbab9351b8fd1aad592023-11-19T00:38:54ZengMDPI AGChemosensors2227-90402023-08-0111846410.3390/chemosensors11080464Quantitative Detection of the Influenza a Virus by an EGOFET-Based Portable DeviceElena Y. Poimanova0Elena G. Zavyalova1Elena A. Kretova2Anton A. Abramov3Askold A. Trul4Oleg V. Borshchev5Anna K. Keshek6Sergey A. Ponomarenko7Elena V. Agina8Enikolopov Institute of Synthetic Polymeric Materials of Russian Academy of Sciences, Profsoyuznaya Str. 70, 117393 Moscow, RussiaEnikolopov Institute of Synthetic Polymeric Materials of Russian Academy of Sciences, Profsoyuznaya Str. 70, 117393 Moscow, RussiaEnikolopov Institute of Synthetic Polymeric Materials of Russian Academy of Sciences, Profsoyuznaya Str. 70, 117393 Moscow, RussiaEnikolopov Institute of Synthetic Polymeric Materials of Russian Academy of Sciences, Profsoyuznaya Str. 70, 117393 Moscow, RussiaEnikolopov Institute of Synthetic Polymeric Materials of Russian Academy of Sciences, Profsoyuznaya Str. 70, 117393 Moscow, RussiaEnikolopov Institute of Synthetic Polymeric Materials of Russian Academy of Sciences, Profsoyuznaya Str. 70, 117393 Moscow, RussiaEnikolopov Institute of Synthetic Polymeric Materials of Russian Academy of Sciences, Profsoyuznaya Str. 70, 117393 Moscow, RussiaEnikolopov Institute of Synthetic Polymeric Materials of Russian Academy of Sciences, Profsoyuznaya Str. 70, 117393 Moscow, RussiaEnikolopov Institute of Synthetic Polymeric Materials of Russian Academy of Sciences, Profsoyuznaya Str. 70, 117393 Moscow, RussiaElaboration of biosensors on the base of organic transistors with embedded biomolecules which can operate in an aqueous environment is of paramount importance. Electrolyte-gated organic field-effect transistors demonstrate high sensitivity in detection of various analytes. In this paper, we demonstrated the possibility of quantitative fast specific determination of virus particles by an aptasensor based on EGOFET. The sensitivity and selectivity of the devices were examined with the influenza A virus as well as with control bioliquids like influenza B, Newcastle disease viruses or allantoic fluid with different dilutions. The influence of the semiconducting layer thickness on EGOFETs sensory properties is discussed. The fabrication of a multi-flow cell that simultaneously registers the responses from several devices on the same substrate and the creation of a multi-sensor flow device are reported. The responses of the elaborated bioelectronic platform to the influenza A virus obtained with application of the portable multi-flow mode are well correlated with the responses obtained in the laboratory stationary mode.https://www.mdpi.com/2227-9040/11/8/464biosensorEGOFETaptamervirus detectionmulti-flow chamber |
| spellingShingle | Elena Y. Poimanova Elena G. Zavyalova Elena A. Kretova Anton A. Abramov Askold A. Trul Oleg V. Borshchev Anna K. Keshek Sergey A. Ponomarenko Elena V. Agina Quantitative Detection of the Influenza a Virus by an EGOFET-Based Portable Device Chemosensors biosensor EGOFET aptamer virus detection multi-flow chamber |
| title | Quantitative Detection of the Influenza a Virus by an EGOFET-Based Portable Device |
| title_full | Quantitative Detection of the Influenza a Virus by an EGOFET-Based Portable Device |
| title_fullStr | Quantitative Detection of the Influenza a Virus by an EGOFET-Based Portable Device |
| title_full_unstemmed | Quantitative Detection of the Influenza a Virus by an EGOFET-Based Portable Device |
| title_short | Quantitative Detection of the Influenza a Virus by an EGOFET-Based Portable Device |
| title_sort | quantitative detection of the influenza a virus by an egofet based portable device |
| topic | biosensor EGOFET aptamer virus detection multi-flow chamber |
| url | https://www.mdpi.com/2227-9040/11/8/464 |
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