Regional Variation in Dengue Virus Serotypes in Sri Lanka and Its Clinical and Epidemiological Relevance
Dengue is a significant health concern in Sri Lanka, but diagnosis of the infecting dengue virus (DENV) serotype has hitherto been largely restricted to the Colombo district in the western province. Salinity tolerant <i>Aedes</i> vectors are present in the island’s northern Jaffna penins...
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MDPI AG
2021-11-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/11/11/2084 |
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| author | Tibutius T. P. Jayadas Thirunavukarasu Kumanan Laksiri Gomes Chandima Jeewandara Gathsaurie N. Malavige Diyanath Ranasinghe Ramesh S. Jadi Ranjan Ramasamy Sinnathamby N. Surendran |
| author_facet | Tibutius T. P. Jayadas Thirunavukarasu Kumanan Laksiri Gomes Chandima Jeewandara Gathsaurie N. Malavige Diyanath Ranasinghe Ramesh S. Jadi Ranjan Ramasamy Sinnathamby N. Surendran |
| author_sort | Tibutius T. P. Jayadas |
| collection | DOAJ |
| description | Dengue is a significant health concern in Sri Lanka, but diagnosis of the infecting dengue virus (DENV) serotype has hitherto been largely restricted to the Colombo district in the western province. Salinity tolerant <i>Aedes</i> vectors are present in the island’s northern Jaffna peninsula, which is undergoing rapid groundwater salinization. Virus serotypes were determined by RT-qPCR in 107 and 112 patients diagnosed by NS1 antigen positivity from the Jaffna district in 2018 and 2019, respectively, and related to clinical characteristics. DENV1 and DENV2 were the most common serotypes in both years. Infections with multiple serotypes were not detected. DENV1 was significantly more prevalent in 2019 than 2018, while DENV3 was significantly more prevalent in 2018 than 2019 among the Jaffna patients. Limited genomic sequencing identified DENV1 genotype-I and DENV3 genotype-I in Jaffna patients in 2018. Dengue was more prevalent in working age persons and males among the serotyped Jaffna patients. DENV1 and DENV2 were the predominant serotypes in 2019 in the Colombo district. However, DENV1 and DENV3 were significantly more prevalent in Colombo compared with Jaffna in 2019. The differences in the prevalence of DENV1 and DENV3 between the Jaffna and Colombo districts in 2019 have implications for dengue epidemiology and vaccination. Salinity-tolerant <i>Aedes</i> vector strains, widespread in the Jaffna peninsula, may have contributed to differences in serotype prevalence compared with the Colombo district in 2019. Significant associations were not identified between virus serotypes and clinical characteristics among Jaffna patients. |
| first_indexed | 2024-03-10T05:34:54Z |
| format | Article |
| id | doaj.art-e582a3d5e66249a2ae17ceac519889a8 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T05:34:54Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-e582a3d5e66249a2ae17ceac519889a82023-11-22T23:02:09ZengMDPI AGDiagnostics2075-44182021-11-011111208410.3390/diagnostics11112084Regional Variation in Dengue Virus Serotypes in Sri Lanka and Its Clinical and Epidemiological RelevanceTibutius T. P. Jayadas0Thirunavukarasu Kumanan1Laksiri Gomes2Chandima Jeewandara3Gathsaurie N. Malavige4Diyanath Ranasinghe5Ramesh S. Jadi6Ranjan Ramasamy7Sinnathamby N. Surendran8Department of Zoology, University of Jaffna, Jaffna 40000, Sri LankaDepartment of Medicine, University of Jaffna, Jaffna 40000, Sri LankaCentre for Dengue Research, Department of Immunology and Molecular Medicine, University of Sri Jayewardenepura, Nugegoda 10250, Sri LankaCentre for Dengue Research, Department of Immunology and Molecular Medicine, University of Sri Jayewardenepura, Nugegoda 10250, Sri LankaCentre for Dengue Research, Department of Immunology and Molecular Medicine, University of Sri Jayewardenepura, Nugegoda 10250, Sri LankaCentre for Dengue Research, Department of Immunology and Molecular Medicine, University of Sri Jayewardenepura, Nugegoda 10250, Sri LankaDepartment of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill, NC 27599-7290, USADepartment of Zoology, University of Jaffna, Jaffna 40000, Sri LankaDepartment of Zoology, University of Jaffna, Jaffna 40000, Sri LankaDengue is a significant health concern in Sri Lanka, but diagnosis of the infecting dengue virus (DENV) serotype has hitherto been largely restricted to the Colombo district in the western province. Salinity tolerant <i>Aedes</i> vectors are present in the island’s northern Jaffna peninsula, which is undergoing rapid groundwater salinization. Virus serotypes were determined by RT-qPCR in 107 and 112 patients diagnosed by NS1 antigen positivity from the Jaffna district in 2018 and 2019, respectively, and related to clinical characteristics. DENV1 and DENV2 were the most common serotypes in both years. Infections with multiple serotypes were not detected. DENV1 was significantly more prevalent in 2019 than 2018, while DENV3 was significantly more prevalent in 2018 than 2019 among the Jaffna patients. Limited genomic sequencing identified DENV1 genotype-I and DENV3 genotype-I in Jaffna patients in 2018. Dengue was more prevalent in working age persons and males among the serotyped Jaffna patients. DENV1 and DENV2 were the predominant serotypes in 2019 in the Colombo district. However, DENV1 and DENV3 were significantly more prevalent in Colombo compared with Jaffna in 2019. The differences in the prevalence of DENV1 and DENV3 between the Jaffna and Colombo districts in 2019 have implications for dengue epidemiology and vaccination. Salinity-tolerant <i>Aedes</i> vector strains, widespread in the Jaffna peninsula, may have contributed to differences in serotype prevalence compared with the Colombo district in 2019. Significant associations were not identified between virus serotypes and clinical characteristics among Jaffna patients.https://www.mdpi.com/2075-4418/11/11/2084clinical manifestations of dengueColombo districtdengue diagnosisdengue epidemiologydengue virus serotypesdengue virus genotypes |
| spellingShingle | Tibutius T. P. Jayadas Thirunavukarasu Kumanan Laksiri Gomes Chandima Jeewandara Gathsaurie N. Malavige Diyanath Ranasinghe Ramesh S. Jadi Ranjan Ramasamy Sinnathamby N. Surendran Regional Variation in Dengue Virus Serotypes in Sri Lanka and Its Clinical and Epidemiological Relevance Diagnostics clinical manifestations of dengue Colombo district dengue diagnosis dengue epidemiology dengue virus serotypes dengue virus genotypes |
| title | Regional Variation in Dengue Virus Serotypes in Sri Lanka and Its Clinical and Epidemiological Relevance |
| title_full | Regional Variation in Dengue Virus Serotypes in Sri Lanka and Its Clinical and Epidemiological Relevance |
| title_fullStr | Regional Variation in Dengue Virus Serotypes in Sri Lanka and Its Clinical and Epidemiological Relevance |
| title_full_unstemmed | Regional Variation in Dengue Virus Serotypes in Sri Lanka and Its Clinical and Epidemiological Relevance |
| title_short | Regional Variation in Dengue Virus Serotypes in Sri Lanka and Its Clinical and Epidemiological Relevance |
| title_sort | regional variation in dengue virus serotypes in sri lanka and its clinical and epidemiological relevance |
| topic | clinical manifestations of dengue Colombo district dengue diagnosis dengue epidemiology dengue virus serotypes dengue virus genotypes |
| url | https://www.mdpi.com/2075-4418/11/11/2084 |
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