Purification, characterization, and immunomodulatory activity of a lectin from the seeds of horse chestnut (Aesculus hippocastanum L.)
This work aimed to purify, characterize, and evaluate the immunomodulatory activity of a lectin from Aesculus hippocastanum seeds (AhSL). The lectin was isolated from saline extract through chromatography on a DEAE-Sephadex column. Protein homogeneity and subunit composition were evaluated with poly...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | Current Research in Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590262822000132 |
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| author | Maxmiliana Fernanda Alves Mariano Soares de Farias Leydianne Leite de Siqueira Patriota Cláudia Bernadete de Souza Lira Lethícia Maria de Souza Aguiar Bárbara Rafaela da Silva Barros Patrícia Maria Guedes Paiva Cristiane Moutinho Lagos de Melo Nataly Diniz de Lima Santos Thiago Henrique Napoleão |
| author_facet | Maxmiliana Fernanda Alves Mariano Soares de Farias Leydianne Leite de Siqueira Patriota Cláudia Bernadete de Souza Lira Lethícia Maria de Souza Aguiar Bárbara Rafaela da Silva Barros Patrícia Maria Guedes Paiva Cristiane Moutinho Lagos de Melo Nataly Diniz de Lima Santos Thiago Henrique Napoleão |
| author_sort | Maxmiliana Fernanda Alves Mariano Soares de Farias |
| collection | DOAJ |
| description | This work aimed to purify, characterize, and evaluate the immunomodulatory activity of a lectin from Aesculus hippocastanum seeds (AhSL). The lectin was isolated from saline extract through chromatography on a DEAE-Sephadex column. Protein homogeneity and subunit composition were evaluated with polyacrylamide gel electrophoresis (PAGE) under native and denaturing (SDS-PAGE) conditions, respectively. AhSL was evaluated for trypsin inhibitor activity, carbohydrate-binding specificity, and the effects of cations (Ca2+, Mn2+, and Mg2+), temperature, and pH on its hemagglutinating activity. Lectin stability under heating and pH variation was evaluated through fluorometric analysis. The activity of this lectin (12.5 µg/mL) in modulating cytokine release and nitric oxide production by mouse splenocytes was assessed. Isolated AhSL (purification fold: 68.8) showed a single polypeptide band in PAGE for acidic proteins and two polypeptide bands of approximately 68 and 124 kDa in SDS-PAGE. Its hemagglutinating activity was inhibited by monosaccharides, disaccharides, and glycoproteins, but was not affected by cations, and remained stable after heating (30–100 °C) and under pH 4.0–8.0. Fluorometric analysis revealed that the overall lectin conformation was not altered after heating to 100 °C and remained stable under various pH values. AhSL also showed trypsin inhibitor activity (specific activity: 1,280.31 U/mg) and induced the release of interleukins 10 and 6, tumor necrosis factor α, and nitric oxide by splenocytes. In conclusion, AhSL is a bioactive protein with promising biotechnological potential, which may find applications as immunological targeting or antitumor agents. |
| first_indexed | 2024-04-11T12:52:20Z |
| format | Article |
| id | doaj.art-e5837dcab4574f8babe070e0724f79d1 |
| institution | Directory Open Access Journal |
| issn | 2590-2628 |
| language | English |
| last_indexed | 2024-04-11T12:52:20Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Biotechnology |
| spelling | doaj.art-e5837dcab4574f8babe070e0724f79d12022-12-22T04:23:11ZengElsevierCurrent Research in Biotechnology2590-26282022-01-014203210Purification, characterization, and immunomodulatory activity of a lectin from the seeds of horse chestnut (Aesculus hippocastanum L.)Maxmiliana Fernanda Alves Mariano Soares de Farias0Leydianne Leite de Siqueira Patriota1Cláudia Bernadete de Souza Lira2Lethícia Maria de Souza Aguiar3Bárbara Rafaela da Silva Barros4Patrícia Maria Guedes Paiva5Cristiane Moutinho Lagos de Melo6Nataly Diniz de Lima Santos7Thiago Henrique Napoleão8Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Cidade Universitária, Recife, Pernambuco, BrazilDepartamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Cidade Universitária, Recife, Pernambuco, BrazilDepartamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Cidade Universitária, Recife, Pernambuco, BrazilDepartamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Cidade Universitária, Recife, Pernambuco, BrazilDepartamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco, Cidade Universitária, Recife, BrazilDepartamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Cidade Universitária, Recife, Pernambuco, BrazilDepartamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco, Cidade Universitária, Recife, BrazilDepartamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Cidade Universitária, Recife, Pernambuco, BrazilDepartamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Cidade Universitária, Recife, Pernambuco, Brazil; Corresponding author.This work aimed to purify, characterize, and evaluate the immunomodulatory activity of a lectin from Aesculus hippocastanum seeds (AhSL). The lectin was isolated from saline extract through chromatography on a DEAE-Sephadex column. Protein homogeneity and subunit composition were evaluated with polyacrylamide gel electrophoresis (PAGE) under native and denaturing (SDS-PAGE) conditions, respectively. AhSL was evaluated for trypsin inhibitor activity, carbohydrate-binding specificity, and the effects of cations (Ca2+, Mn2+, and Mg2+), temperature, and pH on its hemagglutinating activity. Lectin stability under heating and pH variation was evaluated through fluorometric analysis. The activity of this lectin (12.5 µg/mL) in modulating cytokine release and nitric oxide production by mouse splenocytes was assessed. Isolated AhSL (purification fold: 68.8) showed a single polypeptide band in PAGE for acidic proteins and two polypeptide bands of approximately 68 and 124 kDa in SDS-PAGE. Its hemagglutinating activity was inhibited by monosaccharides, disaccharides, and glycoproteins, but was not affected by cations, and remained stable after heating (30–100 °C) and under pH 4.0–8.0. Fluorometric analysis revealed that the overall lectin conformation was not altered after heating to 100 °C and remained stable under various pH values. AhSL also showed trypsin inhibitor activity (specific activity: 1,280.31 U/mg) and induced the release of interleukins 10 and 6, tumor necrosis factor α, and nitric oxide by splenocytes. In conclusion, AhSL is a bioactive protein with promising biotechnological potential, which may find applications as immunological targeting or antitumor agents.http://www.sciencedirect.com/science/article/pii/S2590262822000132Multifunctional proteinCytokinesMedicinal plantThermostable protein |
| spellingShingle | Maxmiliana Fernanda Alves Mariano Soares de Farias Leydianne Leite de Siqueira Patriota Cláudia Bernadete de Souza Lira Lethícia Maria de Souza Aguiar Bárbara Rafaela da Silva Barros Patrícia Maria Guedes Paiva Cristiane Moutinho Lagos de Melo Nataly Diniz de Lima Santos Thiago Henrique Napoleão Purification, characterization, and immunomodulatory activity of a lectin from the seeds of horse chestnut (Aesculus hippocastanum L.) Current Research in Biotechnology Multifunctional protein Cytokines Medicinal plant Thermostable protein |
| title | Purification, characterization, and immunomodulatory activity of a lectin from the seeds of horse chestnut (Aesculus hippocastanum L.) |
| title_full | Purification, characterization, and immunomodulatory activity of a lectin from the seeds of horse chestnut (Aesculus hippocastanum L.) |
| title_fullStr | Purification, characterization, and immunomodulatory activity of a lectin from the seeds of horse chestnut (Aesculus hippocastanum L.) |
| title_full_unstemmed | Purification, characterization, and immunomodulatory activity of a lectin from the seeds of horse chestnut (Aesculus hippocastanum L.) |
| title_short | Purification, characterization, and immunomodulatory activity of a lectin from the seeds of horse chestnut (Aesculus hippocastanum L.) |
| title_sort | purification characterization and immunomodulatory activity of a lectin from the seeds of horse chestnut aesculus hippocastanum l |
| topic | Multifunctional protein Cytokines Medicinal plant Thermostable protein |
| url | http://www.sciencedirect.com/science/article/pii/S2590262822000132 |
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