Ameliorative effect of black ginseng extract against oxidative stress-induced cellular damages in mouse hepatocytes
Background: Oxidative stress induces the production of reactive oxygen species (ROS), which play important causative roles in various pathological conditions. Black ginseng (BG), a type of steam-processed ginseng, has drawn significant attention due to its biological activity, and is more potent tha...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-04-01
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| Series: | Journal of Ginseng Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1226845317302427 |
| _version_ | 1811280690899058688 |
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| author | Qaisra Naheed Choudhry Jun Ho Kim Hyung Taek Cho Wan Heo Jeong-Jun Lee Jin Hyup Lee Young Jun Kim |
| author_facet | Qaisra Naheed Choudhry Jun Ho Kim Hyung Taek Cho Wan Heo Jeong-Jun Lee Jin Hyup Lee Young Jun Kim |
| author_sort | Qaisra Naheed Choudhry |
| collection | DOAJ |
| description | Background: Oxidative stress induces the production of reactive oxygen species (ROS), which play important causative roles in various pathological conditions. Black ginseng (BG), a type of steam-processed ginseng, has drawn significant attention due to its biological activity, and is more potent than white ginseng (WG) or red ginseng (RG). Methods: We evaluated the protective effects of BG extract (BGE) against oxidative stress-induced cellular damage, in comparison with WG extract (WGE) and RG extract (RGE) in a cell culture model. Ethanolic extracts of WG, RG, and BG were used to evaluate ginsenoside profiles, total polyphenols, flavonoid contents, and antioxidant activity. Using AML-12 cells treated with H2O2, the protective effects of WGE, RGE, and BGE on cellular redox status, DNA, protein, lipid damage, and apoptosis levels were investigated. Results: BGE exhibited significantly enhanced antioxidant potential, as well as total flavonoid and polyphenol contents. ATP levels were significantly higher in BGE-treated cells than in control; ROS generation and glutathione disulfide levels were lower but glutathione (GSH) and NADPH levels were higher in BGE-treated cells than in other groups. Pretreatment with BGE inhibited apoptosis and therefore protected cells from oxidative stress-induced cellular damage, probably through ROS scavenging. Conclusion: Collectively, our results demonstrate that BGE protects AML-12 cells from oxidative stress-induced cellular damages more effectively than WGE or RGE, through ROS scavenging, maintenance of redox status, and activation of the antioxidant defense system. Keywords: antioxidant, black ginseng, chemoprotection, oxidative stress, Panax ginseng |
| first_indexed | 2024-04-13T01:19:22Z |
| format | Article |
| id | doaj.art-e586d39b152d484f9330879303620930 |
| institution | Directory Open Access Journal |
| issn | 1226-8453 |
| language | English |
| last_indexed | 2024-04-13T01:19:22Z |
| publishDate | 2019-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Ginseng Research |
| spelling | doaj.art-e586d39b152d484f93308793036209302022-12-22T03:08:50ZengElsevierJournal of Ginseng Research1226-84532019-04-01432179185Ameliorative effect of black ginseng extract against oxidative stress-induced cellular damages in mouse hepatocytesQaisra Naheed Choudhry0Jun Ho Kim1Hyung Taek Cho2Wan Heo3Jeong-Jun Lee4Jin Hyup Lee5Young Jun Kim6Department of Food and Biotechnology, Korea University, Sejong, Republic of KoreaDepartment of Food and Biotechnology, Korea University, Sejong, Republic of KoreaDepartment of Food and Biotechnology, Korea University, Sejong, Republic of KoreaDepartment of Food and Biotechnology, Korea University, Sejong, Republic of KoreaFood Research and Development Center, Naturetech Co., Ltd., Chungbuk, Republic of KoreaDepartment of Food and Biotechnology, Korea University, Sejong, Republic of Korea; Corresponding author. Department of Food and Biotechnology, College of Science and Technology, Korea University, 2511 Sejong-ro, Sejong 30019, Republic of Korea.Department of Food and Biotechnology, Korea University, Sejong, Republic of Korea; Corresponding author. Department of Food and Biotechnology, College of Science and Technology, Korea University, 2511 Sejong-ro, Sejong 30019, Republic of Korea.Background: Oxidative stress induces the production of reactive oxygen species (ROS), which play important causative roles in various pathological conditions. Black ginseng (BG), a type of steam-processed ginseng, has drawn significant attention due to its biological activity, and is more potent than white ginseng (WG) or red ginseng (RG). Methods: We evaluated the protective effects of BG extract (BGE) against oxidative stress-induced cellular damage, in comparison with WG extract (WGE) and RG extract (RGE) in a cell culture model. Ethanolic extracts of WG, RG, and BG were used to evaluate ginsenoside profiles, total polyphenols, flavonoid contents, and antioxidant activity. Using AML-12 cells treated with H2O2, the protective effects of WGE, RGE, and BGE on cellular redox status, DNA, protein, lipid damage, and apoptosis levels were investigated. Results: BGE exhibited significantly enhanced antioxidant potential, as well as total flavonoid and polyphenol contents. ATP levels were significantly higher in BGE-treated cells than in control; ROS generation and glutathione disulfide levels were lower but glutathione (GSH) and NADPH levels were higher in BGE-treated cells than in other groups. Pretreatment with BGE inhibited apoptosis and therefore protected cells from oxidative stress-induced cellular damage, probably through ROS scavenging. Conclusion: Collectively, our results demonstrate that BGE protects AML-12 cells from oxidative stress-induced cellular damages more effectively than WGE or RGE, through ROS scavenging, maintenance of redox status, and activation of the antioxidant defense system. Keywords: antioxidant, black ginseng, chemoprotection, oxidative stress, Panax ginsenghttp://www.sciencedirect.com/science/article/pii/S1226845317302427 |
| spellingShingle | Qaisra Naheed Choudhry Jun Ho Kim Hyung Taek Cho Wan Heo Jeong-Jun Lee Jin Hyup Lee Young Jun Kim Ameliorative effect of black ginseng extract against oxidative stress-induced cellular damages in mouse hepatocytes Journal of Ginseng Research |
| title | Ameliorative effect of black ginseng extract against oxidative stress-induced cellular damages in mouse hepatocytes |
| title_full | Ameliorative effect of black ginseng extract against oxidative stress-induced cellular damages in mouse hepatocytes |
| title_fullStr | Ameliorative effect of black ginseng extract against oxidative stress-induced cellular damages in mouse hepatocytes |
| title_full_unstemmed | Ameliorative effect of black ginseng extract against oxidative stress-induced cellular damages in mouse hepatocytes |
| title_short | Ameliorative effect of black ginseng extract against oxidative stress-induced cellular damages in mouse hepatocytes |
| title_sort | ameliorative effect of black ginseng extract against oxidative stress induced cellular damages in mouse hepatocytes |
| url | http://www.sciencedirect.com/science/article/pii/S1226845317302427 |
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