Dual tRNA mimicry in the Cricket Paralysis Virus IRES uncovers an unexpected similarity with the Hepatitis C Virus IRES
Co-opting the cellular machinery for protein production is a compulsory requirement for viruses. The Cricket Paralysis Virus employs an Internal Ribosomal Entry Site (CrPV-IRES) to express its structural genes in the late stage of infection. Ribosome hijacking is achieved by a sophisticated use of m...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2018-06-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/34062 |
| _version_ | 1797997515013881856 |
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| author | Vera P Pisareva Andrey V Pisarev Israel S Fernández |
| author_facet | Vera P Pisareva Andrey V Pisarev Israel S Fernández |
| author_sort | Vera P Pisareva |
| collection | DOAJ |
| description | Co-opting the cellular machinery for protein production is a compulsory requirement for viruses. The Cricket Paralysis Virus employs an Internal Ribosomal Entry Site (CrPV-IRES) to express its structural genes in the late stage of infection. Ribosome hijacking is achieved by a sophisticated use of molecular mimicry to tRNA and mRNA, employed to manipulate intrinsically dynamic components of the ribosome. Binding and translocation through the ribosome is required for this IRES to initiate translation. We report two structures, solved by single particle electron cryo-microscopy (cryoEM), of a double translocated CrPV-IRES with aminoacyl-tRNA in the peptidyl site (P site) of the ribosome. CrPV-IRES adopts a previously unseen conformation, mimicking the acceptor stem of a canonical E site tRNA. The structures suggest a mechanism for the positioning of the first aminoacyl-tRNA shared with the distantly related Hepatitis C Virus IRES. |
| first_indexed | 2024-04-11T10:34:04Z |
| format | Article |
| id | doaj.art-e5896752877b4aba8d8b053bf2c1b78b |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T10:34:04Z |
| publishDate | 2018-06-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-e5896752877b4aba8d8b053bf2c1b78b2022-12-22T04:29:21ZengeLife Sciences Publications LtdeLife2050-084X2018-06-01710.7554/eLife.34062Dual tRNA mimicry in the Cricket Paralysis Virus IRES uncovers an unexpected similarity with the Hepatitis C Virus IRESVera P Pisareva0Andrey V Pisarev1Israel S Fernández2https://orcid.org/0000-0001-7218-1603Department of Cell Biology, SUNY Downstate Medical Center, Brooklyn, United StatesDepartment of Cell Biology, SUNY Downstate Medical Center, Brooklyn, United StatesDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, United StatesCo-opting the cellular machinery for protein production is a compulsory requirement for viruses. The Cricket Paralysis Virus employs an Internal Ribosomal Entry Site (CrPV-IRES) to express its structural genes in the late stage of infection. Ribosome hijacking is achieved by a sophisticated use of molecular mimicry to tRNA and mRNA, employed to manipulate intrinsically dynamic components of the ribosome. Binding and translocation through the ribosome is required for this IRES to initiate translation. We report two structures, solved by single particle electron cryo-microscopy (cryoEM), of a double translocated CrPV-IRES with aminoacyl-tRNA in the peptidyl site (P site) of the ribosome. CrPV-IRES adopts a previously unseen conformation, mimicking the acceptor stem of a canonical E site tRNA. The structures suggest a mechanism for the positioning of the first aminoacyl-tRNA shared with the distantly related Hepatitis C Virus IRES.https://elifesciences.org/articles/34062rabbitreticulocyteribosome |
| spellingShingle | Vera P Pisareva Andrey V Pisarev Israel S Fernández Dual tRNA mimicry in the Cricket Paralysis Virus IRES uncovers an unexpected similarity with the Hepatitis C Virus IRES eLife rabbit reticulocyte ribosome |
| title | Dual tRNA mimicry in the Cricket Paralysis Virus IRES uncovers an unexpected similarity with the Hepatitis C Virus IRES |
| title_full | Dual tRNA mimicry in the Cricket Paralysis Virus IRES uncovers an unexpected similarity with the Hepatitis C Virus IRES |
| title_fullStr | Dual tRNA mimicry in the Cricket Paralysis Virus IRES uncovers an unexpected similarity with the Hepatitis C Virus IRES |
| title_full_unstemmed | Dual tRNA mimicry in the Cricket Paralysis Virus IRES uncovers an unexpected similarity with the Hepatitis C Virus IRES |
| title_short | Dual tRNA mimicry in the Cricket Paralysis Virus IRES uncovers an unexpected similarity with the Hepatitis C Virus IRES |
| title_sort | dual trna mimicry in the cricket paralysis virus ires uncovers an unexpected similarity with the hepatitis c virus ires |
| topic | rabbit reticulocyte ribosome |
| url | https://elifesciences.org/articles/34062 |
| work_keys_str_mv | AT verappisareva dualtrnamimicryinthecricketparalysisvirusiresuncoversanunexpectedsimilaritywiththehepatitiscvirusires AT andreyvpisarev dualtrnamimicryinthecricketparalysisvirusiresuncoversanunexpectedsimilaritywiththehepatitiscvirusires AT israelsfernandez dualtrnamimicryinthecricketparalysisvirusiresuncoversanunexpectedsimilaritywiththehepatitiscvirusires |