Molecular, serological and biological characterizations of Potato Leaf Roll Virus in infected potato plants in Egypt, and its effects on plant cell organelles
Potato Leaf Roll Virus (PLRV) is one of the most serious viruses infecting potato plant (Solanum tuberosum L.) in Egypt. Indirect Enzyme Linked Immunosorbent Assay (Indirect- ELISA) results revealed that 70 % of the collected samples were infected with PLRV. A multiplex Polymerase Chain Reaction (...
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| Format: | Article |
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The National Information and Documentation Centre (NIDOC) , Egypt
2019-08-01
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| Series: | Novel Research in Microbiology Journal |
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| Online Access: | https://nrmj.journals.ekb.eg/article_44953_16c9dc5407eeb4c837b5320d642e14fd.pdf |
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| author | Dalia G. Aseel Mahmoud H. Abd El-Aziz Sanaa A. Riad Azaa Makhlouf Gaber I. Fegla Elsayed E. Hafez |
| author_facet | Dalia G. Aseel Mahmoud H. Abd El-Aziz Sanaa A. Riad Azaa Makhlouf Gaber I. Fegla Elsayed E. Hafez |
| author_sort | Dalia G. Aseel |
| collection | DOAJ |
| description | Potato Leaf Roll Virus (PLRV) is one of the most serious viruses infecting potato plant (Solanum tuberosum
L.) in Egypt. Indirect Enzyme Linked Immunosorbent Assay (Indirect- ELISA) results revealed that 70 % of the
collected samples were infected with PLRV. A multiplex Polymerase Chain Reaction (PCR) was carried out
using three different sets of primers, specific for both PLRV and Potato virus Y (PVY) isolates. For confirmation;
the movement coat protein (MP) gene was isolated from the infected plant tissues, and a band with molecular size
336 bp was obtained using Reverse transcription-Polymerase chain reaction (RT-PCR). The DNA sequence of the
Egyptian PLRV-Banha -MP gene was deposited in GenBank under an accession number of KR002119.
Moreover, sequence analysis revealed that the Egyptian PLRV isolate was closely related to a New Zealand
isolate of PLRV (GU002341), with identity of 100%. Transmission electron microscope (TEM) examination of
PLRV showed isometric particles, with approximate size of 24-30 nm. The cytopathological examination of the
potato plant infected with PLRV revealed many cellular effects such as; partially degraded and deformed
chloroplast, starch with an increased size and color change, in addition to nucleus and cytoplasmic bridge. It could
be concluded that PLRV is present in Egypt, infecting most of the potato cultivars. Moreover, four different
strains of PLRV were detected based on the Single strand conformation polymorphism (SSCP) of the MP gene.
The aims of the current study were to identify the PLRV infection of potato plant in Egypt using; molecular,
serological and biological methods, in addition to studying the effect of this virus on potato cell organelles. This is
the first record of the presence of four different strains of PLRV infecting potato in Egypt, using SSCP assay. |
| first_indexed | 2024-04-24T08:17:40Z |
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| id | doaj.art-e58beae813594b0890185b119a8a40a6 |
| institution | Directory Open Access Journal |
| issn | 2537-0286 2537-0294 |
| language | English |
| last_indexed | 2024-04-24T08:17:40Z |
| publishDate | 2019-08-01 |
| publisher | The National Information and Documentation Centre (NIDOC) , Egypt |
| record_format | Article |
| series | Novel Research in Microbiology Journal |
| spelling | doaj.art-e58beae813594b0890185b119a8a40a62024-04-17T03:19:23ZengThe National Information and Documentation Centre (NIDOC) , EgyptNovel Research in Microbiology Journal2537-02862537-02942019-08-013445346310.21608/nrmj.2019.44953Molecular, serological and biological characterizations of Potato Leaf Roll Virus in infected potato plants in Egypt, and its effects on plant cell organellesDalia G. Aseel0Mahmoud H. Abd El-Aziz1Sanaa A. Riad2Azaa Makhlouf3Gaber I. Fegla4Elsayed E. Hafez5Plant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technology Applications, Borg EL-Arab city, Alexandria, EgyptPlant Pathology Institute, Agriculture Research Center, Alexandria, EgyptGenetics Department, Faculty of Agriculture, Alexandria University, Alexandria, EgyptGenetics Department, Faculty of Agriculture, Alexandria University, Alexandria, EgyptPlant Pathology Department, Faculty of Agriculture, Alexandria University, Alexandria, EgyptPlant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technology Applications, Borg EL-Arab city, Alexandria, EgyptPotato Leaf Roll Virus (PLRV) is one of the most serious viruses infecting potato plant (Solanum tuberosum L.) in Egypt. Indirect Enzyme Linked Immunosorbent Assay (Indirect- ELISA) results revealed that 70 % of the collected samples were infected with PLRV. A multiplex Polymerase Chain Reaction (PCR) was carried out using three different sets of primers, specific for both PLRV and Potato virus Y (PVY) isolates. For confirmation; the movement coat protein (MP) gene was isolated from the infected plant tissues, and a band with molecular size 336 bp was obtained using Reverse transcription-Polymerase chain reaction (RT-PCR). The DNA sequence of the Egyptian PLRV-Banha -MP gene was deposited in GenBank under an accession number of KR002119. Moreover, sequence analysis revealed that the Egyptian PLRV isolate was closely related to a New Zealand isolate of PLRV (GU002341), with identity of 100%. Transmission electron microscope (TEM) examination of PLRV showed isometric particles, with approximate size of 24-30 nm. The cytopathological examination of the potato plant infected with PLRV revealed many cellular effects such as; partially degraded and deformed chloroplast, starch with an increased size and color change, in addition to nucleus and cytoplasmic bridge. It could be concluded that PLRV is present in Egypt, infecting most of the potato cultivars. Moreover, four different strains of PLRV were detected based on the Single strand conformation polymorphism (SSCP) of the MP gene. The aims of the current study were to identify the PLRV infection of potato plant in Egypt using; molecular, serological and biological methods, in addition to studying the effect of this virus on potato cell organelles. This is the first record of the presence of four different strains of PLRV infecting potato in Egypt, using SSCP assay.https://nrmj.journals.ekb.eg/article_44953_16c9dc5407eeb4c837b5320d642e14fd.pdfpotatoplrvindirect-elisamultiplex pcrsscptem |
| spellingShingle | Dalia G. Aseel Mahmoud H. Abd El-Aziz Sanaa A. Riad Azaa Makhlouf Gaber I. Fegla Elsayed E. Hafez Molecular, serological and biological characterizations of Potato Leaf Roll Virus in infected potato plants in Egypt, and its effects on plant cell organelles Novel Research in Microbiology Journal potato plrv indirect-elisa multiplex pcr sscp tem |
| title | Molecular, serological and biological characterizations of Potato Leaf Roll Virus in infected potato plants in Egypt, and its effects on plant cell organelles |
| title_full | Molecular, serological and biological characterizations of Potato Leaf Roll Virus in infected potato plants in Egypt, and its effects on plant cell organelles |
| title_fullStr | Molecular, serological and biological characterizations of Potato Leaf Roll Virus in infected potato plants in Egypt, and its effects on plant cell organelles |
| title_full_unstemmed | Molecular, serological and biological characterizations of Potato Leaf Roll Virus in infected potato plants in Egypt, and its effects on plant cell organelles |
| title_short | Molecular, serological and biological characterizations of Potato Leaf Roll Virus in infected potato plants in Egypt, and its effects on plant cell organelles |
| title_sort | molecular serological and biological characterizations of potato leaf roll virus in infected potato plants in egypt and its effects on plant cell organelles |
| topic | potato plrv indirect-elisa multiplex pcr sscp tem |
| url | https://nrmj.journals.ekb.eg/article_44953_16c9dc5407eeb4c837b5320d642e14fd.pdf |
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