Isolation and identification of phytoestrogens and flavonoids in an Ayurvedic proprietary medicine using chromatographic and Mass Spectroscopic analysis
Objective: To develop analytical methods for the isolation and structural identification of poly phenols including phytoestrogens in Mensokot tablet, a herbal proprietary medicine. Methods: Isolation consisted of an ultrasound-assisted extraction, followed by acid hydrolysis and a final liquid-liqui...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-06-01
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| Series: | Asian Pacific Journal of Reproduction |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2305050015300130 |
| _version_ | 1811312386708078592 |
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| author | CT Sulaiman A Arun EM Anandan CR Sandhya Indira Balachandran |
| author_facet | CT Sulaiman A Arun EM Anandan CR Sandhya Indira Balachandran |
| author_sort | CT Sulaiman |
| collection | DOAJ |
| description | Objective: To develop analytical methods for the isolation and structural identification of poly phenols including phytoestrogens in Mensokot tablet, a herbal proprietary medicine.
Methods: Isolation consisted of an ultrasound-assisted extraction, followed by acid hydrolysis and a final liquid-liquid extraction step in diethyl ether. Identification and structural characterisation was done by liquid chromatography coupled with Q-TOF-ESI-MS/MS analysis.
Results: Phytoestrogens such as Coumestrol, Genistein and Glycitein have been identified in Mensokot tablet along with several other flavonoids.
Conclusions: In the present research, a rapid HPLC-MS/MS method has been developed for the identification of phytoestrogens and other flavonoids from an Ayurvedic proprietary medicine. Phytoestrogens are considered to play an important role in the prevention of cancers, heart disease, menopausal symptoms and osteoporosis. |
| first_indexed | 2024-04-13T10:35:24Z |
| format | Article |
| id | doaj.art-e590782fc61c4eb4a8a8a86d93e131d1 |
| institution | Directory Open Access Journal |
| issn | 2305-0500 |
| language | English |
| last_indexed | 2024-04-13T10:35:24Z |
| publishDate | 2015-06-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Reproduction |
| spelling | doaj.art-e590782fc61c4eb4a8a8a86d93e131d12022-12-22T02:50:04ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Reproduction2305-05002015-06-014215315610.1016/S2305-0500(15)30013-0Isolation and identification of phytoestrogens and flavonoids in an Ayurvedic proprietary medicine using chromatographic and Mass Spectroscopic analysisCT Sulaiman0A Arun1EM Anandan2CR Sandhya3Indira Balachandran4Phytochemistry Division, Centre for Medicinal Plants Research, Arya Vaidya sala, Kottakkal, IndiaProduct Development Department, Arya Vaidya Sala, Kottakkal, IndiaProduct Development Department, Arya Vaidya Sala, Kottakkal, IndiaProduct Development Department, Arya Vaidya Sala, Kottakkal, IndiaPhytochemistry Division, Centre for Medicinal Plants Research, Arya Vaidya sala, Kottakkal, IndiaObjective: To develop analytical methods for the isolation and structural identification of poly phenols including phytoestrogens in Mensokot tablet, a herbal proprietary medicine. Methods: Isolation consisted of an ultrasound-assisted extraction, followed by acid hydrolysis and a final liquid-liquid extraction step in diethyl ether. Identification and structural characterisation was done by liquid chromatography coupled with Q-TOF-ESI-MS/MS analysis. Results: Phytoestrogens such as Coumestrol, Genistein and Glycitein have been identified in Mensokot tablet along with several other flavonoids. Conclusions: In the present research, a rapid HPLC-MS/MS method has been developed for the identification of phytoestrogens and other flavonoids from an Ayurvedic proprietary medicine. Phytoestrogens are considered to play an important role in the prevention of cancers, heart disease, menopausal symptoms and osteoporosis.http://www.sciencedirect.com/science/article/pii/S2305050015300130PhytoestrogensHerbal medicineIsoflavonesLC/MS |
| spellingShingle | CT Sulaiman A Arun EM Anandan CR Sandhya Indira Balachandran Isolation and identification of phytoestrogens and flavonoids in an Ayurvedic proprietary medicine using chromatographic and Mass Spectroscopic analysis Asian Pacific Journal of Reproduction Phytoestrogens Herbal medicine Isoflavones LC/MS |
| title | Isolation and identification of phytoestrogens and flavonoids in an Ayurvedic proprietary medicine using chromatographic and Mass Spectroscopic analysis |
| title_full | Isolation and identification of phytoestrogens and flavonoids in an Ayurvedic proprietary medicine using chromatographic and Mass Spectroscopic analysis |
| title_fullStr | Isolation and identification of phytoestrogens and flavonoids in an Ayurvedic proprietary medicine using chromatographic and Mass Spectroscopic analysis |
| title_full_unstemmed | Isolation and identification of phytoestrogens and flavonoids in an Ayurvedic proprietary medicine using chromatographic and Mass Spectroscopic analysis |
| title_short | Isolation and identification of phytoestrogens and flavonoids in an Ayurvedic proprietary medicine using chromatographic and Mass Spectroscopic analysis |
| title_sort | isolation and identification of phytoestrogens and flavonoids in an ayurvedic proprietary medicine using chromatographic and mass spectroscopic analysis |
| topic | Phytoestrogens Herbal medicine Isoflavones LC/MS |
| url | http://www.sciencedirect.com/science/article/pii/S2305050015300130 |
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