Molecular characterization and identification of fungi causing stern canker of oilseed rape in Serbia
During 2008-2010 the infected Brassica napus plant material was collected from nine localities: Karavukovo, Crvenka, Prigrevica, Subotica, Rimski Šančevi, Srbobran, Beška, Banatsko Karađorđevo, and Srpski Miletić. Infected tissue samples were taken from the root of both upper and basal stems, as wel...
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| Format: | Article |
| Language: | English |
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Institute of field and vegetable crops, Novi Sad
2017-01-01
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| Series: | Ratarstvo i Povrtarstvo |
| Subjects: | |
| Online Access: | http://scindeks-clanci.ceon.rs/data/pdf/1821-3944/2017/1821-39441702056M.pdf |
| _version_ | 1828419593744416768 |
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| author | Mitrović Petar Marjanović-Jeromel Ana Milovac Željko Radić Velimir |
| author_facet | Mitrović Petar Marjanović-Jeromel Ana Milovac Željko Radić Velimir |
| author_sort | Mitrović Petar |
| collection | DOAJ |
| description | During 2008-2010 the infected Brassica napus plant material was collected from nine localities: Karavukovo, Crvenka, Prigrevica, Subotica, Rimski Šančevi, Srbobran, Beška, Banatsko Karađorđevo, and Srpski Miletić. Infected tissue samples were taken from the root of both upper and basal stems, as well as from the leaf, flower and pods. Two reference isolates from Great Britain were used in these studies for the analysis of 119 isolates from Serbia, using polymerase chain reaction (PCR) and PCR-RFLP. Digestion of PCR products was performed with 5 selected endonucleases: BamHI, HaeIII, Rsal, EcoRII, and AluI. On the basis of PCR analysis, all isolates originating from Serbia belong to L. complex: 111 belong to species Leptosphaeria maculans, and 8 to species Leptosphaeria biglobosa NA1 ('Leptosphaeria biglobosa brassicae), respectively. |
| first_indexed | 2024-12-10T14:56:40Z |
| format | Article |
| id | doaj.art-e592c9a70a124542a264ef1b3d1ff4e7 |
| institution | Directory Open Access Journal |
| issn | 1821-3944 2217-8392 |
| language | English |
| last_indexed | 2024-12-10T14:56:40Z |
| publishDate | 2017-01-01 |
| publisher | Institute of field and vegetable crops, Novi Sad |
| record_format | Article |
| series | Ratarstvo i Povrtarstvo |
| spelling | doaj.art-e592c9a70a124542a264ef1b3d1ff4e72022-12-22T01:44:17ZengInstitute of field and vegetable crops, Novi SadRatarstvo i Povrtarstvo1821-39442217-83922017-01-01542566010.5937/ratpov54-126831821-39441702056MMolecular characterization and identification of fungi causing stern canker of oilseed rape in SerbiaMitrović Petar0Marjanović-Jeromel Ana1Milovac Željko2Radić Velimir3Institute of Field and Vegetable Crops, Novi Sad, SerbiaInstitute of Field and Vegetable Crops, Novi Sad, SerbiaInstitute of Field and Vegetable Crops, Novi Sad, SerbiaInstitute of Field and Vegetable Crops, Novi Sad, SerbiaDuring 2008-2010 the infected Brassica napus plant material was collected from nine localities: Karavukovo, Crvenka, Prigrevica, Subotica, Rimski Šančevi, Srbobran, Beška, Banatsko Karađorđevo, and Srpski Miletić. Infected tissue samples were taken from the root of both upper and basal stems, as well as from the leaf, flower and pods. Two reference isolates from Great Britain were used in these studies for the analysis of 119 isolates from Serbia, using polymerase chain reaction (PCR) and PCR-RFLP. Digestion of PCR products was performed with 5 selected endonucleases: BamHI, HaeIII, Rsal, EcoRII, and AluI. On the basis of PCR analysis, all isolates originating from Serbia belong to L. complex: 111 belong to species Leptosphaeria maculans, and 8 to species Leptosphaeria biglobosa NA1 ('Leptosphaeria biglobosa brassicae), respectively.http://scindeks-clanci.ceon.rs/data/pdf/1821-3944/2017/1821-39441702056M.pdfBrassica napusendonucleasesisolatesLeptosphaeria maculansLeptosphaeria biglobosalocalitiesPCR and PCR-RFLP |
| spellingShingle | Mitrović Petar Marjanović-Jeromel Ana Milovac Željko Radić Velimir Molecular characterization and identification of fungi causing stern canker of oilseed rape in Serbia Ratarstvo i Povrtarstvo Brassica napus endonucleases isolates Leptosphaeria maculans Leptosphaeria biglobosa localities PCR and PCR-RFLP |
| title | Molecular characterization and identification of fungi causing stern canker of oilseed rape in Serbia |
| title_full | Molecular characterization and identification of fungi causing stern canker of oilseed rape in Serbia |
| title_fullStr | Molecular characterization and identification of fungi causing stern canker of oilseed rape in Serbia |
| title_full_unstemmed | Molecular characterization and identification of fungi causing stern canker of oilseed rape in Serbia |
| title_short | Molecular characterization and identification of fungi causing stern canker of oilseed rape in Serbia |
| title_sort | molecular characterization and identification of fungi causing stern canker of oilseed rape in serbia |
| topic | Brassica napus endonucleases isolates Leptosphaeria maculans Leptosphaeria biglobosa localities PCR and PCR-RFLP |
| url | http://scindeks-clanci.ceon.rs/data/pdf/1821-3944/2017/1821-39441702056M.pdf |
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