Differences in the Gut Microbiota Composition and Metabolites Associated With Feeding Intolerance in VLBW Infants With a Gestational Age of ≤ 30 Weeks: A Pilot Study
ObjectiveTo explore the main variations in gut microbiota compositions, short-chain fatty acids (SCFAs) concentrations and autoinducer-2 (AI-2) levels in very-low-birth-weight (VLBW) infants with feeding intolerance (FI).MethodsTwenty-seven VLBW infants with gestational ages of ≤30 weeks were divide...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-02-01
|
| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.726322/full |
| _version_ | 1818294280376025088 |
|---|---|
| author | Xiao-Chen Liu Xiao-Chen Liu Xiao-Chen Liu Xiao-Chen Liu Xiao-Chen Liu Qian Sun Qian Sun Qian Sun Qian Sun Qian Sun Yan-Chun Ji Yan-Chun Ji Yan-Chun Ji Yan-Chun Ji Yan-Chun Ji Li-Zhen Fu Li-Zhen Fu Li-Zhen Fu Li-Zhen Fu Li-Zhen Fu Zheng-Li Wang Zheng-Li Wang Zheng-Li Wang Zheng-Li Wang Zheng-Li Wang Yu He Yu He Yu He Yu He Yu He Lu-Quan Li Lu-Quan Li Lu-Quan Li Lu-Quan Li Lu-Quan Li |
| author_facet | Xiao-Chen Liu Xiao-Chen Liu Xiao-Chen Liu Xiao-Chen Liu Xiao-Chen Liu Qian Sun Qian Sun Qian Sun Qian Sun Qian Sun Yan-Chun Ji Yan-Chun Ji Yan-Chun Ji Yan-Chun Ji Yan-Chun Ji Li-Zhen Fu Li-Zhen Fu Li-Zhen Fu Li-Zhen Fu Li-Zhen Fu Zheng-Li Wang Zheng-Li Wang Zheng-Li Wang Zheng-Li Wang Zheng-Li Wang Yu He Yu He Yu He Yu He Yu He Lu-Quan Li Lu-Quan Li Lu-Quan Li Lu-Quan Li Lu-Quan Li |
| author_sort | Xiao-Chen Liu |
| collection | DOAJ |
| description | ObjectiveTo explore the main variations in gut microbiota compositions, short-chain fatty acids (SCFAs) concentrations and autoinducer-2 (AI-2) levels in very-low-birth-weight (VLBW) infants with feeding intolerance (FI).MethodsTwenty-seven VLBW infants with gestational ages of ≤30 weeks were divided into the FI group (n=14) and feeding tolerance (FT) group (n=13). The gut microbiota composition and SCFAs concentrations and AI-2 levels in feces were detected at 2 and 4 weeks after birth.ResultsThere was no difference in alpha diversity between the two groups at 2 and 4 weeks after birth (P>0.05). Although the Chao index decreased (P<0.05), there was no difference in the Shannon index from 2 weeks to 4 weeks in either the FI or FT group (P>0.05). Additionally, there was no difference in beta diversity between the FI and FT groups at 2 weeks (P>0.05), but there was a significant difference in beta diversity between the two groups at 4 weeks (P<0.05) and a large difference from 2 weeks to 4 weeks in both the FI and FT groups (P<0.05). Furthermore, the composition of the microbiota at 4 weeks was significantly different from that at 2 weeks in the FI group (P<0.05). The Veillonella abundance was lower at 4 weeks in the FI group (P<0.05), but there were no differences in the compositions of the other main microbes between the two groups (P>0.05). Proteobacteria and Firmicutes were dominant in both the FI and FT groups. The concentrations of propanoic, valeric and hexanoic acids were lower in the FI group at 2 weeks, and the levels of isobutyric and valeric acids were lower at 4 weeks after birth (P<0.05). The areas under the curves (AUCs) of propanoic, butanoic and valeric acids in predicting FI were 0.878, 0.816 and 0.744, respectively. Compared with that in the FT group, the relative bioluminescence of AI-2 was lower in the FI group at 2 weeks (P<0.05), and the AUC was 0.736.ConclusionsThe main composition of the microbiota was not obviously different in infants with FI. Some SCFAs and AI-2 have moderate value in predicting FI. |
| first_indexed | 2024-12-13T03:29:14Z |
| format | Article |
| id | doaj.art-e595afe1da7646a7bb2205d4ab390df3 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-13T03:29:14Z |
| publishDate | 2022-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-e595afe1da7646a7bb2205d4ab390df32022-12-22T00:01:12ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-02-011210.3389/fcimb.2022.726322726322Differences in the Gut Microbiota Composition and Metabolites Associated With Feeding Intolerance in VLBW Infants With a Gestational Age of ≤ 30 Weeks: A Pilot StudyXiao-Chen Liu0Xiao-Chen Liu1Xiao-Chen Liu2Xiao-Chen Liu3Xiao-Chen Liu4Qian Sun5Qian Sun6Qian Sun7Qian Sun8Qian Sun9Yan-Chun Ji10Yan-Chun Ji11Yan-Chun Ji12Yan-Chun Ji13Yan-Chun Ji14Li-Zhen Fu15Li-Zhen Fu16Li-Zhen Fu17Li-Zhen Fu18Li-Zhen Fu19Zheng-Li Wang20Zheng-Li Wang21Zheng-Li Wang22Zheng-Li Wang23Zheng-Li Wang24Yu He25Yu He26Yu He27Yu He28Yu He29Lu-Quan Li30Lu-Quan Li31Lu-Quan Li32Lu-Quan Li33Lu-Quan Li34Neonatal Diagnosis and Treatment Centre of Children’s Hospital of Chongqing Medical University, Chongqing, ChinaNational Clinical Research Center for Child Health and Disorders, Chongqing, ChinaMinistry of Education Key Laboratory of Child Development and Disorders, Chongqing, ChinaChina International Science and Technology Cooperation base of Child Development and Critical Disorders, Chongqing, ChinaChongqing Key Laboratory of Pediatrics, Chongqing, ChinaNeonatal Diagnosis and Treatment Centre of Children’s Hospital of Chongqing Medical University, Chongqing, ChinaNational Clinical Research Center for Child Health and Disorders, Chongqing, ChinaMinistry of Education Key Laboratory of Child Development and Disorders, Chongqing, ChinaChina International Science and Technology Cooperation base of Child Development and Critical Disorders, Chongqing, ChinaChongqing Key Laboratory of Pediatrics, Chongqing, ChinaNeonatal Diagnosis and Treatment Centre of Children’s Hospital of Chongqing Medical University, Chongqing, ChinaNational Clinical Research Center for Child Health and Disorders, Chongqing, ChinaMinistry of Education Key Laboratory of Child Development and Disorders, Chongqing, ChinaChina International Science and Technology Cooperation base of Child Development and Critical Disorders, Chongqing, ChinaChongqing Key Laboratory of Pediatrics, Chongqing, ChinaNeonatal Diagnosis and Treatment Centre of Children’s Hospital of Chongqing Medical University, Chongqing, ChinaNational Clinical Research Center for Child Health and Disorders, Chongqing, ChinaMinistry of Education Key Laboratory of Child Development and Disorders, Chongqing, ChinaChina International Science and Technology Cooperation base of Child Development and Critical Disorders, Chongqing, ChinaChongqing Key Laboratory of Pediatrics, Chongqing, ChinaNeonatal Diagnosis and Treatment Centre of Children’s Hospital of Chongqing Medical University, Chongqing, ChinaNational Clinical Research Center for Child Health and Disorders, Chongqing, ChinaMinistry of Education Key Laboratory of Child Development and Disorders, Chongqing, ChinaChina International Science and Technology Cooperation base of Child Development and Critical Disorders, Chongqing, ChinaChongqing Key Laboratory of Pediatrics, Chongqing, ChinaNeonatal Diagnosis and Treatment Centre of Children’s Hospital of Chongqing Medical University, Chongqing, ChinaNational Clinical Research Center for Child Health and Disorders, Chongqing, ChinaMinistry of Education Key Laboratory of Child Development and Disorders, Chongqing, ChinaChina International Science and Technology Cooperation base of Child Development and Critical Disorders, Chongqing, ChinaChongqing Key Laboratory of Pediatrics, Chongqing, ChinaNeonatal Diagnosis and Treatment Centre of Children’s Hospital of Chongqing Medical University, Chongqing, ChinaNational Clinical Research Center for Child Health and Disorders, Chongqing, ChinaMinistry of Education Key Laboratory of Child Development and Disorders, Chongqing, ChinaChina International Science and Technology Cooperation base of Child Development and Critical Disorders, Chongqing, ChinaChongqing Key Laboratory of Pediatrics, Chongqing, ChinaObjectiveTo explore the main variations in gut microbiota compositions, short-chain fatty acids (SCFAs) concentrations and autoinducer-2 (AI-2) levels in very-low-birth-weight (VLBW) infants with feeding intolerance (FI).MethodsTwenty-seven VLBW infants with gestational ages of ≤30 weeks were divided into the FI group (n=14) and feeding tolerance (FT) group (n=13). The gut microbiota composition and SCFAs concentrations and AI-2 levels in feces were detected at 2 and 4 weeks after birth.ResultsThere was no difference in alpha diversity between the two groups at 2 and 4 weeks after birth (P>0.05). Although the Chao index decreased (P<0.05), there was no difference in the Shannon index from 2 weeks to 4 weeks in either the FI or FT group (P>0.05). Additionally, there was no difference in beta diversity between the FI and FT groups at 2 weeks (P>0.05), but there was a significant difference in beta diversity between the two groups at 4 weeks (P<0.05) and a large difference from 2 weeks to 4 weeks in both the FI and FT groups (P<0.05). Furthermore, the composition of the microbiota at 4 weeks was significantly different from that at 2 weeks in the FI group (P<0.05). The Veillonella abundance was lower at 4 weeks in the FI group (P<0.05), but there were no differences in the compositions of the other main microbes between the two groups (P>0.05). Proteobacteria and Firmicutes were dominant in both the FI and FT groups. The concentrations of propanoic, valeric and hexanoic acids were lower in the FI group at 2 weeks, and the levels of isobutyric and valeric acids were lower at 4 weeks after birth (P<0.05). The areas under the curves (AUCs) of propanoic, butanoic and valeric acids in predicting FI were 0.878, 0.816 and 0.744, respectively. Compared with that in the FT group, the relative bioluminescence of AI-2 was lower in the FI group at 2 weeks (P<0.05), and the AUC was 0.736.ConclusionsThe main composition of the microbiota was not obviously different in infants with FI. Some SCFAs and AI-2 have moderate value in predicting FI.https://www.frontiersin.org/articles/10.3389/fcimb.2022.726322/fullfeeding intolerancegut microbiotashort-chain fatty acids (SCFAs)autoinducer-2 (AI-2)metabolites |
| spellingShingle | Xiao-Chen Liu Xiao-Chen Liu Xiao-Chen Liu Xiao-Chen Liu Xiao-Chen Liu Qian Sun Qian Sun Qian Sun Qian Sun Qian Sun Yan-Chun Ji Yan-Chun Ji Yan-Chun Ji Yan-Chun Ji Yan-Chun Ji Li-Zhen Fu Li-Zhen Fu Li-Zhen Fu Li-Zhen Fu Li-Zhen Fu Zheng-Li Wang Zheng-Li Wang Zheng-Li Wang Zheng-Li Wang Zheng-Li Wang Yu He Yu He Yu He Yu He Yu He Lu-Quan Li Lu-Quan Li Lu-Quan Li Lu-Quan Li Lu-Quan Li Differences in the Gut Microbiota Composition and Metabolites Associated With Feeding Intolerance in VLBW Infants With a Gestational Age of ≤ 30 Weeks: A Pilot Study Frontiers in Cellular and Infection Microbiology feeding intolerance gut microbiota short-chain fatty acids (SCFAs) autoinducer-2 (AI-2) metabolites |
| title | Differences in the Gut Microbiota Composition and Metabolites Associated With Feeding Intolerance in VLBW Infants With a Gestational Age of ≤ 30 Weeks: A Pilot Study |
| title_full | Differences in the Gut Microbiota Composition and Metabolites Associated With Feeding Intolerance in VLBW Infants With a Gestational Age of ≤ 30 Weeks: A Pilot Study |
| title_fullStr | Differences in the Gut Microbiota Composition and Metabolites Associated With Feeding Intolerance in VLBW Infants With a Gestational Age of ≤ 30 Weeks: A Pilot Study |
| title_full_unstemmed | Differences in the Gut Microbiota Composition and Metabolites Associated With Feeding Intolerance in VLBW Infants With a Gestational Age of ≤ 30 Weeks: A Pilot Study |
| title_short | Differences in the Gut Microbiota Composition and Metabolites Associated With Feeding Intolerance in VLBW Infants With a Gestational Age of ≤ 30 Weeks: A Pilot Study |
| title_sort | differences in the gut microbiota composition and metabolites associated with feeding intolerance in vlbw infants with a gestational age of ≤ 30 weeks a pilot study |
| topic | feeding intolerance gut microbiota short-chain fatty acids (SCFAs) autoinducer-2 (AI-2) metabolites |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.726322/full |
| work_keys_str_mv | AT xiaochenliu differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT xiaochenliu differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT xiaochenliu differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT xiaochenliu differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT xiaochenliu differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT qiansun differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT qiansun differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT qiansun differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT qiansun differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT qiansun differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT yanchunji differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT yanchunji differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT yanchunji differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT yanchunji differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT yanchunji differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT lizhenfu differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT lizhenfu differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT lizhenfu differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT lizhenfu differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT lizhenfu differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT zhengliwang differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT zhengliwang differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT zhengliwang differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT zhengliwang differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT zhengliwang differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT yuhe differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT yuhe differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT yuhe differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT yuhe differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT yuhe differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT luquanli differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT luquanli differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT luquanli differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT luquanli differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy AT luquanli differencesinthegutmicrobiotacompositionandmetabolitesassociatedwithfeedingintoleranceinvlbwinfantswithagestationalageof30weeksapilotstudy |