Assessment of Inhibition of Biofilm Formation on Non-Thermal Plasma-Treated TiO<sub>2</sub> Nanotubes
Peri-implantitis is an inflammatory disease similar to periodontitis, caused by biofilms formed on the surface of dental implants. This inflammation can spread to bone tissues and result in bone loss. Therefore, it is essential to inhibit the formation of biofilms on the surface of dental implants....
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MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/4/3335 |
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| author | Min-Kyung Ji Seon-Ki Lee Hee-Seon Kim Gye-Jeong Oh Hoonsung Cho Hyun-Pil Lim |
| author_facet | Min-Kyung Ji Seon-Ki Lee Hee-Seon Kim Gye-Jeong Oh Hoonsung Cho Hyun-Pil Lim |
| author_sort | Min-Kyung Ji |
| collection | DOAJ |
| description | Peri-implantitis is an inflammatory disease similar to periodontitis, caused by biofilms formed on the surface of dental implants. This inflammation can spread to bone tissues and result in bone loss. Therefore, it is essential to inhibit the formation of biofilms on the surface of dental implants. Thus, this study examined the inhibition of biofilm formation by treating TiO<sub>2</sub> nanotubes with heat and plasma. Commercially pure titanium specimens were anodized to form TiO<sub>2</sub> nanotubes. Heat treatment was performed at 400 and 600 °C, and atmospheric pressure plasma was applied using a plasma generator (PGS-200, Expantech, Suwon, Republic of Korea). Contact angles, surface roughness, surface structure, crystal structure, and chemical compositions were measured to analyze the surface properties of the specimens. The inhibition of biofilm formation was assessed using two methods. The results of this study showed that the heat treatment of TiO<sub>2</sub> nanotubes at 400 °C inhibited the adhesion of <i>Streptococcus mutans</i> (<i>S. mutans</i>), associated with initial biofilm formation, and that heat treatment of TiO<sub>2</sub> nanotubes at 600 °C inhibited the adhesion of <i>Porphyromonas gingivalis</i> (<i>P. gingivalis</i>), which causes peri-implantitis. Applying plasma to the TiO<sub>2</sub> nanotubes heat-treated at 600 °C inhibited the adhesion of <i>S. mutans</i> and <i>P. gingivalis.</i> |
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| last_indexed | 2024-03-11T08:44:28Z |
| publishDate | 2023-02-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e595e7f404864c50af925e6c1ad0c9a42023-11-16T20:58:33ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01244333510.3390/ijms24043335Assessment of Inhibition of Biofilm Formation on Non-Thermal Plasma-Treated TiO<sub>2</sub> NanotubesMin-Kyung Ji0Seon-Ki Lee1Hee-Seon Kim2Gye-Jeong Oh3Hoonsung Cho4Hyun-Pil Lim5Dental 4D Research Center, Chonnam National University, 33 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaDepartment of Prosthodontics, Daejeon Dental Hospital, Wonkwang University, 77 Dunsan-ro, Seo-gu, Daejeon 35233, Republic of KoreaDepartment of Prosthodontics, School of Dentistry, Chonnam National University, 33 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaBiomedical Evaluation & Research Center, Chonnam National University, 33 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaSchool of Materials Science & Engineering, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaDepartment of Prosthodontics, School of Dentistry, Chonnam National University, 33 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of KoreaPeri-implantitis is an inflammatory disease similar to periodontitis, caused by biofilms formed on the surface of dental implants. This inflammation can spread to bone tissues and result in bone loss. Therefore, it is essential to inhibit the formation of biofilms on the surface of dental implants. Thus, this study examined the inhibition of biofilm formation by treating TiO<sub>2</sub> nanotubes with heat and plasma. Commercially pure titanium specimens were anodized to form TiO<sub>2</sub> nanotubes. Heat treatment was performed at 400 and 600 °C, and atmospheric pressure plasma was applied using a plasma generator (PGS-200, Expantech, Suwon, Republic of Korea). Contact angles, surface roughness, surface structure, crystal structure, and chemical compositions were measured to analyze the surface properties of the specimens. The inhibition of biofilm formation was assessed using two methods. The results of this study showed that the heat treatment of TiO<sub>2</sub> nanotubes at 400 °C inhibited the adhesion of <i>Streptococcus mutans</i> (<i>S. mutans</i>), associated with initial biofilm formation, and that heat treatment of TiO<sub>2</sub> nanotubes at 600 °C inhibited the adhesion of <i>Porphyromonas gingivalis</i> (<i>P. gingivalis</i>), which causes peri-implantitis. Applying plasma to the TiO<sub>2</sub> nanotubes heat-treated at 600 °C inhibited the adhesion of <i>S. mutans</i> and <i>P. gingivalis.</i>https://www.mdpi.com/1422-0067/24/4/3335TiO<sub>2</sub> nanotubeheat treatmentnon-thermal plasma treatmentperi-implantitisbiofilms |
| spellingShingle | Min-Kyung Ji Seon-Ki Lee Hee-Seon Kim Gye-Jeong Oh Hoonsung Cho Hyun-Pil Lim Assessment of Inhibition of Biofilm Formation on Non-Thermal Plasma-Treated TiO<sub>2</sub> Nanotubes International Journal of Molecular Sciences TiO<sub>2</sub> nanotube heat treatment non-thermal plasma treatment peri-implantitis biofilms |
| title | Assessment of Inhibition of Biofilm Formation on Non-Thermal Plasma-Treated TiO<sub>2</sub> Nanotubes |
| title_full | Assessment of Inhibition of Biofilm Formation on Non-Thermal Plasma-Treated TiO<sub>2</sub> Nanotubes |
| title_fullStr | Assessment of Inhibition of Biofilm Formation on Non-Thermal Plasma-Treated TiO<sub>2</sub> Nanotubes |
| title_full_unstemmed | Assessment of Inhibition of Biofilm Formation on Non-Thermal Plasma-Treated TiO<sub>2</sub> Nanotubes |
| title_short | Assessment of Inhibition of Biofilm Formation on Non-Thermal Plasma-Treated TiO<sub>2</sub> Nanotubes |
| title_sort | assessment of inhibition of biofilm formation on non thermal plasma treated tio sub 2 sub nanotubes |
| topic | TiO<sub>2</sub> nanotube heat treatment non-thermal plasma treatment peri-implantitis biofilms |
| url | https://www.mdpi.com/1422-0067/24/4/3335 |
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