Development of an Immunoassay Method for the Sensitive Detection of Histamine and Tryptamine in Foods Based on a CuO@Au Nanoenzyme Label and Molecularly Imprinted Biomimetic Antibody
In this paper, a novel biomimetic enzyme-linked immunoassay method (BELISA) was successfully established for the detection of histamine and tryptamine, based on catalytically active cupric oxide@gold nanoparticles (CuO@Au NPs) as a marker and a molecularly imprinted polymer (MIP) as the biomimetic a...
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MDPI AG
2022-12-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/15/1/21 |
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| author | Xinli Peng Yongfeng Chen Chunhui Gao Yufeng Sun Geoffrey I. N. Waterhouse Zhixiang Xu |
| author_facet | Xinli Peng Yongfeng Chen Chunhui Gao Yufeng Sun Geoffrey I. N. Waterhouse Zhixiang Xu |
| author_sort | Xinli Peng |
| collection | DOAJ |
| description | In this paper, a novel biomimetic enzyme-linked immunoassay method (BELISA) was successfully established for the detection of histamine and tryptamine, based on catalytically active cupric oxide@gold nanoparticles (CuO@Au NPs) as a marker and a molecularly imprinted polymer (MIP) as the biomimetic antibody. Under optimized conditions, the detection limitations of the BELISA method for histamine and tryptamine were 0.04 mg L<sup>−1</sup> and 0.14 mg L<sup>−1</sup>, respectively. For liquor spiked with histamine and tryptamine, the BELISA method delivered satisfactory recoveries ranging from 89.90% to 115.00%. Furthermore, the levels of histamine and tryptamine in fish, soy sauce, and rice vinegar samples were detected by the BELISA method and a high performance liquid chromatography method, with no significant difference between the two methods being found. Although the catalytic activity of nanozymes is still lower than that of natural enzymes, the BELISA method could still sensitively determine the histamine and tryptamine levels in food samples. |
| first_indexed | 2024-03-09T03:27:52Z |
| format | Article |
| id | doaj.art-e597642cd4a64bab87b25571f2560a15 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T03:27:52Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-e597642cd4a64bab87b25571f2560a152023-12-03T15:00:59ZengMDPI AGPolymers2073-43602022-12-011512110.3390/polym15010021Development of an Immunoassay Method for the Sensitive Detection of Histamine and Tryptamine in Foods Based on a CuO@Au Nanoenzyme Label and Molecularly Imprinted Biomimetic AntibodyXinli Peng0Yongfeng Chen1Chunhui Gao2Yufeng Sun3Geoffrey I. N. Waterhouse4Zhixiang Xu5College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaCollege of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaCollege of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaCollege of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaSchool of Chemical Sciences, The University of Auckland, Auckland 1142, New ZealandCollege of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaIn this paper, a novel biomimetic enzyme-linked immunoassay method (BELISA) was successfully established for the detection of histamine and tryptamine, based on catalytically active cupric oxide@gold nanoparticles (CuO@Au NPs) as a marker and a molecularly imprinted polymer (MIP) as the biomimetic antibody. Under optimized conditions, the detection limitations of the BELISA method for histamine and tryptamine were 0.04 mg L<sup>−1</sup> and 0.14 mg L<sup>−1</sup>, respectively. For liquor spiked with histamine and tryptamine, the BELISA method delivered satisfactory recoveries ranging from 89.90% to 115.00%. Furthermore, the levels of histamine and tryptamine in fish, soy sauce, and rice vinegar samples were detected by the BELISA method and a high performance liquid chromatography method, with no significant difference between the two methods being found. Although the catalytic activity of nanozymes is still lower than that of natural enzymes, the BELISA method could still sensitively determine the histamine and tryptamine levels in food samples.https://www.mdpi.com/2073-4360/15/1/21histaminetryptaminenanozymebiomimetic immunoassay |
| spellingShingle | Xinli Peng Yongfeng Chen Chunhui Gao Yufeng Sun Geoffrey I. N. Waterhouse Zhixiang Xu Development of an Immunoassay Method for the Sensitive Detection of Histamine and Tryptamine in Foods Based on a CuO@Au Nanoenzyme Label and Molecularly Imprinted Biomimetic Antibody Polymers histamine tryptamine nanozyme biomimetic immunoassay |
| title | Development of an Immunoassay Method for the Sensitive Detection of Histamine and Tryptamine in Foods Based on a CuO@Au Nanoenzyme Label and Molecularly Imprinted Biomimetic Antibody |
| title_full | Development of an Immunoassay Method for the Sensitive Detection of Histamine and Tryptamine in Foods Based on a CuO@Au Nanoenzyme Label and Molecularly Imprinted Biomimetic Antibody |
| title_fullStr | Development of an Immunoassay Method for the Sensitive Detection of Histamine and Tryptamine in Foods Based on a CuO@Au Nanoenzyme Label and Molecularly Imprinted Biomimetic Antibody |
| title_full_unstemmed | Development of an Immunoassay Method for the Sensitive Detection of Histamine and Tryptamine in Foods Based on a CuO@Au Nanoenzyme Label and Molecularly Imprinted Biomimetic Antibody |
| title_short | Development of an Immunoassay Method for the Sensitive Detection of Histamine and Tryptamine in Foods Based on a CuO@Au Nanoenzyme Label and Molecularly Imprinted Biomimetic Antibody |
| title_sort | development of an immunoassay method for the sensitive detection of histamine and tryptamine in foods based on a cuo au nanoenzyme label and molecularly imprinted biomimetic antibody |
| topic | histamine tryptamine nanozyme biomimetic immunoassay |
| url | https://www.mdpi.com/2073-4360/15/1/21 |
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