Protein Abundance Control by Non-coding Antisense Transcription
Stable unannotated transcripts (SUTs), some of which overlap protein-coding genes in antisense direction, are a class of non-coding RNAs. While case studies have reported important regulatory roles for several of such RNAs, their general impact on protein abundance regulation of the overlapping gene...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016-06-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221112471630626X |
| _version_ | 1818303006356013056 |
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| author | Florian Huber Daria Bunina Ishaan Gupta Anton Khmelinskii Matthias Meurer Patrick Theer Lars M. Steinmetz Michael Knop |
| author_facet | Florian Huber Daria Bunina Ishaan Gupta Anton Khmelinskii Matthias Meurer Patrick Theer Lars M. Steinmetz Michael Knop |
| author_sort | Florian Huber |
| collection | DOAJ |
| description | Stable unannotated transcripts (SUTs), some of which overlap protein-coding genes in antisense direction, are a class of non-coding RNAs. While case studies have reported important regulatory roles for several of such RNAs, their general impact on protein abundance regulation of the overlapping gene is not known. To test this, we employed seamless gene manipulation to repress antisense SUTs of 162 yeast genes by using a unidirectional transcriptional terminator and a GFP tag. We found that the mere presence of antisense SUTs was not sufficient to influence protein abundance, that observed effects of antisense SUTs correlated with sense transcript start site overlap, and that the effects were generally weak and led to reduced protein levels. Antisense regulated genes showed increased H3K4 di- and trimethylation and had slightly lower than expected noise levels. Our results suggest that the functionality of antisense RNAs has gene and condition-specific components. |
| first_indexed | 2024-12-13T05:47:56Z |
| format | Article |
| id | doaj.art-e59889bda649405db0cd993dfe372a4a |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-13T05:47:56Z |
| publishDate | 2016-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-e59889bda649405db0cd993dfe372a4a2022-12-21T23:57:37ZengElsevierCell Reports2211-12472016-06-0115122625263610.1016/j.celrep.2016.05.043Protein Abundance Control by Non-coding Antisense TranscriptionFlorian Huber0Daria Bunina1Ishaan Gupta2Anton Khmelinskii3Matthias Meurer4Patrick Theer5Lars M. Steinmetz6Michael Knop7Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), University of Heidelberg, Im Neuenheimer Feld 282, 69120 Heidelberg, GermanyZentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), University of Heidelberg, Im Neuenheimer Feld 282, 69120 Heidelberg, GermanyGenome Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstraße 1, 69117 Heidelberg, GermanyZentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), University of Heidelberg, Im Neuenheimer Feld 282, 69120 Heidelberg, GermanyZentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), University of Heidelberg, Im Neuenheimer Feld 282, 69120 Heidelberg, GermanyZentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), University of Heidelberg, Im Neuenheimer Feld 282, 69120 Heidelberg, GermanyGenome Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstraße 1, 69117 Heidelberg, GermanyZentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), University of Heidelberg, Im Neuenheimer Feld 282, 69120 Heidelberg, GermanyStable unannotated transcripts (SUTs), some of which overlap protein-coding genes in antisense direction, are a class of non-coding RNAs. While case studies have reported important regulatory roles for several of such RNAs, their general impact on protein abundance regulation of the overlapping gene is not known. To test this, we employed seamless gene manipulation to repress antisense SUTs of 162 yeast genes by using a unidirectional transcriptional terminator and a GFP tag. We found that the mere presence of antisense SUTs was not sufficient to influence protein abundance, that observed effects of antisense SUTs correlated with sense transcript start site overlap, and that the effects were generally weak and led to reduced protein levels. Antisense regulated genes showed increased H3K4 di- and trimethylation and had slightly lower than expected noise levels. Our results suggest that the functionality of antisense RNAs has gene and condition-specific components.http://www.sciencedirect.com/science/article/pii/S221112471630626X |
| spellingShingle | Florian Huber Daria Bunina Ishaan Gupta Anton Khmelinskii Matthias Meurer Patrick Theer Lars M. Steinmetz Michael Knop Protein Abundance Control by Non-coding Antisense Transcription Cell Reports |
| title | Protein Abundance Control by Non-coding Antisense Transcription |
| title_full | Protein Abundance Control by Non-coding Antisense Transcription |
| title_fullStr | Protein Abundance Control by Non-coding Antisense Transcription |
| title_full_unstemmed | Protein Abundance Control by Non-coding Antisense Transcription |
| title_short | Protein Abundance Control by Non-coding Antisense Transcription |
| title_sort | protein abundance control by non coding antisense transcription |
| url | http://www.sciencedirect.com/science/article/pii/S221112471630626X |
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